Volume 9, Issue 1 (Vol.9 No.1 Apr 2020)                   rbmb.net 2020, 9(1): 106-114 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ariaee N, Sankian M, Varasteh A, Moghadam M, Jabbari F. Introducing a Stabilizer Formulation for Allergenic Mold Extracts. rbmb.net. 2020; 9 (1) :106-114
URL: http://rbmb.net/article-1-422-en.html
Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (391 Views)
Background: Sensitization to common mold allergens is one of the major causes of allergic rhinitis and asthma. Therefore, there is a critical need for standard sensitivity tests including skin prick tests to improve the stability of fungi extracts in traditional allergenic formulations. To address this concern, the present study aimed to develop a formulation to preserve allergenic activity of mold extracts.

Methods: 48 stabilizer formulations were designed and monitored for allergenic activity during a 40-days incubation period at 37 ˚C using an ELISA. Specifically, the IgE reactivity of allergenic A. alternata extracts were examined. After establishing the most effective stabilizer formulation, we evaluated whether it could protect the allergenic activity of Alt a1, A. fumigatus, and C. herbarum using an IgE inhibition ELISA after 40 days at 37 ˚C.

Results: We demonstrated that the most effective stabilizer formulation was a glycerol-based extract containing Arg and Glu. This formulation had an equal ratio of sucrose, sorbitol and protein and was able to preserve more than 95% of allergenic A. alternata extract activity during a 40-days incubation period at 37 ˚C.

Conclusions: The present study reveals a novel formulation that is an efficient stabilizer of allergenic mold extract activity and has practical applications in mold skin prick tests, ELISAs, immunotherapies, and RAST.
Full-Text [PDF 269 kb]   (124 Downloads)    
Type of Article: Original Article | Subject: Immunology
Received: 2019/11/12 | Accepted: 2020/01/18 | Published: 2020/07/18

References
1. Rapiejko P, Jurkiewicz D, Pietruszewska W, Zielnik-Jurkiewicz B, Woron J, Lipiec A. Treatment strategy of allergic rhinitis in the face of modern world threats. Otolaryngol Pol. 2018;72(2):1-12. [DOI:10.5604/01.3001.0011.8057] [PMID]
2. Seidman MD, Gurgel RK, Lin SY, Schwartz SR, Baroody FM, Bonner JR, et al. Clinical practice guideline: allergic rhinitis. Otolaryngology Head Neck Surg. 2015;152(1 suppl):S1-43.
3. Cardona V, Demoly P, Dreborg S, Kalpaklioglu AF, Klimek L, Muraro A, et al. Current practice of allergy diagnosis and the potential impact of regulation in Europe. Allergy. 2018;73(2):323-327. [DOI:10.1111/all.13306] [PMID]
4. Kołodziejczyk K, Bożek A, Jarząb J, Gawlik R. The clinical differences of asthma in patients with molds allergy. Pneumonol Alergol Pol. 2016;84(2):81-6. [DOI:10.5603/PiAP.2016.0005] [PMID]
5. Fukutomi Y, Taniguchi M. Sensitization to fungal allergens: resolved and unresolved issues. Allergol Int. 2015;64(4):321-31. [DOI:10.1016/j.alit.2015.05.007] [PMID]
6. Twaroch TE, Curin M, Valenta R, Swoboda I. Mold allergens in respiratory allergy: from structure to therapy. Allergy Asthma Immunol Res. 2015;7(3):205-20. [DOI:10.4168/aair.2015.7.3.205] [PMID] [PMCID]
7. Cox L, Nelson H, Lockey R. Allergen immunotherapy: a practice parameter third update. J Allergy Clin Immunol. 2011;127(1 suppl):S1-55. [DOI:10.1016/j.jaci.2010.09.034] [PMID]
8. Raulf M, Quirce S, Vandenplas O. Addressing molecular diagnosis of occupational allergies. Curr Allergy Asthma Rep. 2018;18(1):6. [DOI:10.1007/s11882-018-0759-9] [PMID] [PMCID]
9. Hamilton RG. Assessment of human allergic diseases. Clinical Immunology. 2019:1283-95.
10. Ariaee N, Varasteh A, Jabbari Azad F, Sankian M. Preparation allergenic pollen extracts; the points should be considered to make high-quality products. J Immunoassay Immunochem. 2018:40(1):26-39. [DOI:10.1080/15321819.2018.1543705] [PMID]
11. Plunkett G. Update: stability of allergen extracts to establish expiration dating. Curr Opin Otolaryngol Head Neck Surg. 2016;24(3):261-9. [DOI:10.1097/MOO.0000000000000248] [PMID]
12. Grier TJ, Hall DM, Duncan EA, Gada SM. Allergen stabilities and compatibilities in immunotherapy mixtures that contain cat, dog, dust mite, and cockroach extracts. Ann Allergy Asthma Immunol. 2015;115(6):496-502. [DOI:10.1016/j.anai.2015.10.002] [PMID]
13. Oskouei YM, Hosseini RF, Ahanchian H, Jarahi L, Ariaee N, Azad FJ. Report of common aeroallergens among allergic patients in northeastern Iran. Iran J Otorhinolaryngol. 2017;29(91):89-94.
14. Golovanov AP, Hautbergue GM, Wilson SA, Lian LY. A simple method for improving protein solubility and long-term stability. J Am Chem Soc. 2004;126(29):8933-9. [DOI:10.1021/ja049297h] [PMID]
15. Pawar S, Dell AI, Savage VM, Knies JL. Real versus artificial variation in the thermal sensitivity of biological traits. The American Naturalist. 2016;187(2): E41-52. [DOI:10.1086/684590] [PMID]
16. Zhuang Y, Dreskin SC. Redefining the major peanut allergens. Immunol Res. 2013;55(1-3):125-34. [DOI:10.1007/s12026-012-8355-x] [PMID] [PMCID]
17. Dellavalle PD, Cabrera A, Alem D, Larrañaga P, Ferreira F, Dalla Rizza M. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean journal of agricultural research. 2011;71(2):231-239. [DOI:10.4067/S0718-58392011000200008]
18. Paris S, Fitting C, Ramirez E, Latge JP, David B. Comparison of different extraction methods of Alternaria allergens. Journal of allergy and clinical immunology. 1990;85(5):941-8. [DOI:10.1016/0091-6749(90)90081-E]
19. Ariaee N, Varasteh A, Jabbari Azad F, Sankian M. Preparation allergenic pollen extracts; the points should be considered to make high-quality products. J Immunoassay Immunochem. 2019;40(1):26-39. [DOI:10.1080/15321819.2018.1543705] [PMID]
20. Mansouritorghabeh H, Sankian M, Varasteh A-R, Moghadam M, Khameneh B, Farid-Hosseini R, et al. Determination of Optimum Excipients for Platanus orientalis Pollen Extract by Accelerating Chemical Stability Test and Their Synergistic Effect. Reports of Biochemistry and Molecular Biology. 2019;7(2):189-195.
21. Plunkett G. Stability of allergen extracts used in skin testing and immunotherapy. Curr Opin Otolaryngol Head Neck Surg. 2008;16(3):285-91. [DOI:10.1097/MOO.0b013e3282ff62b0] [PMID]
22. Bijli K, Singh B, Sridhara S, Gaur S, Arora N. Effect of various stabilizing agents on Imperata cylindrica grass pollen allergen extract. Clin Exp Allergy. 2003;33(1):65-71. [DOI:10.1046/j.1365-2222.2003.01554.x] [PMID]
23. Ariaee N, Sankian M, Varasteh A, Hosseinpour M, Jabbari F. Determining if Amino Acids Can Affect Stabilizing Allergenic Pollen Extracts. Reports of Biochemistry & Molecular Biology. 2020;8(4):394-400.
24. Platts L, Darby SJ, Falconer RJ. Control of Globular Protein Thermal Stability in Aqueous Formulations by the Positively Charged Amino Acid Excipients. J Pharm Sci. 2016;105(12):3532-3536. [DOI:10.1016/j.xphs.2016.09.013] [PMID]
25. Esch RE. Manufacturing and standardizing fungal allergen products. J Allergy Clin Immunol. 2004;113(2):210-5. [DOI:10.1016/j.jaci.2003.11.024] [PMID]
26. Gasilova N, Girault HH. Bioanalytical methods for food allergy diagnosis, allergen detection and new allergen discovery. Bioanalysis. 2015;7(9):1175-90. [DOI:10.4155/bio.15.49] [PMID]
27. Hoseini RF, Jabbari F, Rezaee A, Rafatpanah H, Yousefzadeh H, Ariaee N, et al. House dust mite sublingual-swallow immunotherapy in perennial rhinitis: a double-blind, placebo-controlled Iranian study. J Biol Regul Homeost Agents. 2018;32(1):83-88.
28. Zimmer J, Vieths S, Kaul S. Standardization and regulation of allergen products in the European :union:. Curr Allergy Asthma Rep. 2016;16(3):21. [DOI:10.1007/s11882-016-0599-4] [PMID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2015 All Rights Reserved | Reports of Biochemistry and Molecular Biology

Designed & Developed by : Yektaweb