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Asouri M, Alinejad Rokni H, Sahraian M A, Fattahi S, Motamed N, Doosti R, et al . Analysis of Single Nucleotide Polymorphisms in HLA-DRA, IL2RA, and HMGB1 Genes in Multiple Sclerosis. rbmb.net 2020; 9 (2) :199-208
URL: http://rbmb.net/article-1-459-en.html
URL: http://rbmb.net/article-1-459-en.html
Analysis of Single Nucleotide Polymorphisms in HLA-DRA, IL2RA, and HMGB1 Genes in Multiple Sclerosis
Mohsen Asouri 
, Hamid Alinejad Rokni , Mohammad Ali Sahraian , Sadegh Fattahi , Nima Motamed , Rozita Doosti , Hamzeh Rahimi , Maryam Lotfi , Azam Moslemi , Morteza Karimpoor , Fereidoun Mahboudi * , Haleh Akhavan-Niaki
, Hamid Alinejad Rokni , Mohammad Ali Sahraian , Sadegh Fattahi , Nima Motamed , Rozita Doosti , Hamzeh Rahimi , Maryam Lotfi , Azam Moslemi , Morteza Karimpoor , Fereidoun Mahboudi * , Haleh Akhavan-Niaki
Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract: (3350 Views)
Background: Multiple sclerosis (MS) is a common demyelinating neurodegenerative disorder with significant heritability. Previous studies have associated genetic variants in human leukocyte antigen (HLA) complex, IL2RA, and HMGB1 genes with the pathophysiology of MS.
Methods: In order to investigate the gene association in the Iranian population, we performed a genotyping study of 36 variants in the mentioned genes using Sanger sequencing in 102 MS patients and 113 healthy controls.
Results: Our results identified significant associations as well as significant allele frequency differences in some of the studied single-nucleotide polymorphisms including rs4935356, rs3177928, and rs7197 from HLA-DRA gene, and rs12722489 and rs12722490 variants from IL2RA gene (p< 0.05). Moreover, the strong linkage disequilibrium of two common haplotypes was estimated from the HLA-DRA gene.
Conclusions: This association study may suggest the role of these polymorphisms in the genetic susceptibility of MS in the Iranian population and would facilitate the recognition of causative variants in this disease.
Methods: In order to investigate the gene association in the Iranian population, we performed a genotyping study of 36 variants in the mentioned genes using Sanger sequencing in 102 MS patients and 113 healthy controls.
Results: Our results identified significant associations as well as significant allele frequency differences in some of the studied single-nucleotide polymorphisms including rs4935356, rs3177928, and rs7197 from HLA-DRA gene, and rs12722489 and rs12722490 variants from IL2RA gene (p< 0.05). Moreover, the strong linkage disequilibrium of two common haplotypes was estimated from the HLA-DRA gene.
Conclusions: This association study may suggest the role of these polymorphisms in the genetic susceptibility of MS in the Iranian population and would facilitate the recognition of causative variants in this disease.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2020/02/3 | Accepted: 2020/02/23 | Published: 2020/10/7
Received: 2020/02/3 | Accepted: 2020/02/23 | Published: 2020/10/7
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