Reports of Biochemistry
and Molecular Biology
Sat, Apr 20, 2024
[Archive]
Volume 9, Issue 3 (Vol.9 No.3 Oct 2020)
rbmb.net 2020, 9(3): 309-314 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Behshood P, Tajbakhsh E, Momtaz H. Recognition of (Sesc) for Easy Identification of Staphylococcus Epidermidis and Molecular and Phenotypic Study of Β-Lactam Resistance in Staphylococcus Epidermidis Isolates in Isfahan. rbmb.net 2020; 9 (3) :309-314
URL: http://rbmb.net/article-1-480-en.html
URL: http://rbmb.net/article-1-480-en.html
Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract: (2632 Views)
Background: Not only is it crucial to rapidly detect Staphylococcus epidermidis (S. epidermidis) isolates from a broad range of bacteria, but recognizing resistance agents can greatly improve current diagnostic and therapeutic strategies.
Methods: The current cross-sectional study investigated 120 clinical isolates from a nosocomial S. epidermidis infection. The isolates were identified using common biochemical tests, and specific S. epidermidis surface protein C (SesC) primers were used to confirm the presence of S. epidermidis. PCR and special primers were used to detect the β-lactamase gene (blaZ). Methicillin resistance was measured using the agar screening method and antibiotic susceptibility was measured by disk diffusion.
Results: 100 samples were characterized as S. epidermidis using a phenotypic and genotypic methods. From the 100 specimens examined, 80% contained blaZ. According to agar screening, 60% of isolates were methicillin-resistant. S. epidermidis isolates demonstrated the highest resistance to penicillin (93%) and the highest sensitivity to cefazolin (39%).
Conclusions: The increased resistance to β-lactam antibiotics in S. epidermidis isolates is alarming, and certain precautions should be taken by healthcare systems to continuously monitor the antimicrobial pattern of S. epidermidis, so that an appropriate drug treatment can be established.
Methods: The current cross-sectional study investigated 120 clinical isolates from a nosocomial S. epidermidis infection. The isolates were identified using common biochemical tests, and specific S. epidermidis surface protein C (SesC) primers were used to confirm the presence of S. epidermidis. PCR and special primers were used to detect the β-lactamase gene (blaZ). Methicillin resistance was measured using the agar screening method and antibiotic susceptibility was measured by disk diffusion.
Results: 100 samples were characterized as S. epidermidis using a phenotypic and genotypic methods. From the 100 specimens examined, 80% contained blaZ. According to agar screening, 60% of isolates were methicillin-resistant. S. epidermidis isolates demonstrated the highest resistance to penicillin (93%) and the highest sensitivity to cefazolin (39%).
Conclusions: The increased resistance to β-lactam antibiotics in S. epidermidis isolates is alarming, and certain precautions should be taken by healthcare systems to continuously monitor the antimicrobial pattern of S. epidermidis, so that an appropriate drug treatment can be established.
Type of Article: Original Article |
Subject:
Microbiology
Received: 2020/03/26 | Accepted: 2020/05/7 | Published: 2020/12/1
Received: 2020/03/26 | Accepted: 2020/05/7 | Published: 2020/12/1
References
1. Rahimi F, Mehmandoost J, danesh m. misidentification of common pathogenic bacteria in a university hospital laboratory. 2016;21:49-54.
2. Fard SS, Amin M, Khodaparast L, Khodaparast L, Choghakabodi PM, Shahrooei M. Detection of biofilm phenotype of isolated Staphylococcus epidermidis from respiratory catheters of hospitalized patients and evaluation the effect of antibodies against SesC protein on biofilm formation. Clinical Microbiology: Open Access. 2015.
3. Järvinen A-K, Laakso S, Piiparinen P, Aittakorpi A, Lindfors M, Huopaniemi L, et al. Rapid identification of bacterial pathogens using a PCR-and microarray-based assay. BMC microbiology. 2009;9:161. [DOI:10.1186/1471-2180-9-161] [PMID] [PMCID]
4. Kasavandi A, BikhofTorbati M, Amini K. Molecular Detection of Staphylococcus aureus, S. epidermidis, and S. hominis Isolated from the Neonatal Umbilical Cord by Multiplex PCR Method. J Ardabil Uniof Med Sci. 2018;18(3):377-386. [DOI:10.29252/jarums.18.3.377]
5. Khodaparast L, Khodaparast L, Shahrooei M, Stijlemans B, Merckx R, Baatsen P, et al. The possible role of Staphylococcus epidermidi LPxTG surface protein SesC in biofilm formation. PLoS One. 2016;11(1):e0146704. [DOI:10.1371/journal.pone.0146704] [PMID] [PMCID]
6. Tahmasebi h, bokaeian m, adabi j. Phenotypic and molecular study of beta-lactam resistance in coagulasenegative staphylococci samples. 2016:55-53. [DOI:10.29252/jmj.14.1.55]
7. Dehghani A, Gholipour A, Nazari-Vanani R, Heibati F. Molecular investigation of methicillin-resistant Staphylococcus epidermidis and Staphylococcus saprophyticus isolated in Shahrekord training hospitals. J Shahrekord Uuniv Med Sci. 2015;17(2):93-104.
8. Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci. Clinical microbiology reviews. 2014;27(4):870-926. [DOI:10.1128/CMR.00109-13] [PMID] [PMCID]
9. Bush K. Past and present perspectives on β-lactamases. Antimicrobial agents and chemotherapy. 2018;62(10). [DOI:10.1128/AAC.01076-18] [PMID] [PMCID]
10. Xu Z, Mkrtchyan HV, Cutler RR. Antibiotic resistance and mecA characterization of coagulase-negative staphylococci isolated from three hotels in London, UK. Front Microbiol. 2015;6:947. [DOI:10.3389/fmicb.2015.00947] [PMID] [PMCID]
11. Martins A, Riboli DFM, Camargo CH, Pereira VC, de Almeida Sampaio R, de Souza MdLR. Antimicrobial resistance and persistence of Staphylococcus epidermidis clones in a Brazilian university hospital. Diagnostic microbiology and infectious disease. 2013;77(2):164-168. [DOI:10.1016/j.diagmicrobio.2013.06.005] [PMID]
12. Razavi S, Dadashi M, Pormohammad A, Khoramrooz SS, Mirzaii M, Gholipour A, et al. Methicillin-resistant staphylococcus epidermidis in Iran: A systematic review and meta-analysis. Arch Clin Infec Dis. 2018;13(4):e58410. [DOI:10.5812/archcid.58410]
13. Feßler AT, Billerbeck C, Kadlec K, Schwarz S. Identification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis. J Antimicrob Chemother. 2010;65(8):1576-82. [DOI:10.1093/jac/dkq172] [PMID]
14. Park JY, Fox LK, Seo KS, McGuire MA, Park YH, Rurangirwa FR, et al. Comparison of phenotypic and genotypic methods for the species identification of coagulase-negative staphylococcal isolates from bovine intramammary infections. Vet microbiol. 2011;147(1-2):142-8. [DOI:10.1016/j.vetmic.2010.06.020] [PMID] [PMCID]
15. Zehra A, Singh R, Kaur S, Gill J. Molecular characterization of antibiotic-resistant Staphylococcus aureus from livestock (bovine and swine). Vet world. 2017;10(6):598-604. [DOI:10.14202/vetworld.2017.598-604] [PMID] [PMCID]
16. Shamansouri S, Karbasizade V, Khozaie M. Determining Sccmec Types In Staphylococcus Epidermidis Isolated From Clinical Samples Of Ishahan, Iran. Acta Medica Mediterranea. 2016;32:2107-2113.
17. Kitti T, Seng R, Saiprom N, Thummeepak R, Chantratita N, Boonlao C, et al. Molecular Characteristics of Methicillin-Resistant Staphylococci Clinical Isolates from a Tertiary Hospital in Northern Thailand. Can J Infect Dis Med Microbiol. 2018:8457012. [DOI:10.1155/2018/8457012] [PMID] [PMCID]
18. Khodaparast L, Khodaparast L, Van Mellaert L, Shahrooei M, Van Ranst M, Van Eldere J. sesC as a genetic marker for easy identification of Staphylococcus epidermidis from other isolates. Infect Genet Evol. 2016;43:222-4. [DOI:10.1016/j.meegid.2016.05.037] [PMID]
19. Nahaei MR, Shahmohammadi MR, Ebrahimi S, Milani M. Detection of methicillin-resistant coagulase-negative staphylococci and surveillance of antibacterial resistance in a multi-center study from Iran. Jundishapur J Mic. 2015;8(8):e19945. [DOI:10.5812/jjm.19945v2] [PMID] [PMCID]
20. Du X, Zhu Y, Song Y, Li T, Luo T, Sun G, et al. Molecular analysis of Staphylococcus epidermidis strains isolated from community and hospital environments in China. PloS one. 2013;8(5):e62742. [DOI:10.1371/journal.pone.0062742] [PMID] [PMCID]
21. Raei F, Eftekhar F. Studying the presence of blaZ gene and β-lactamase production in clinical isolates of Staphylococcus epidermidis. Iran J Med Microbiol. 2008;2(2):35-41.
22. Akbariyeh H, Nahaei MR, Hasani A, Pormohammad A. Intrinsic and Acquired Methicillin-Resistance Detection in Staphylococcus aureus and Its Relevance in Therapeutics. Archives of Pediatric Infectious Diseases. 2017;5(1):e39185. [DOI:10.5812/pedinfect.39185]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
