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Volume 9, Issue 3 (Vol.9 No.3 Oct 2020)
rbmb.net 2020, 9(3): 357-365 |
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Salimi M, Shirazi A, Norouzian* M, Jafari A, Edalatkhah H, Mehravar M, et al . H19/Igf2 Expression and Methylation of Histone 3 in Mice Chimeric Blastocysts. rbmb.net 2020; 9 (3) :357-365
URL: http://rbmb.net/article-1-525-en.html
URL: http://rbmb.net/article-1-525-en.html
Maryam Salimi 
, Abolfazl Shirazi * , Mohsen Norouzian* , Ameneh Jafari , Haleh Edalatkhah , Maryam Mehravar , Mohammad Majidi , Mohammad Mehdi Mehrazar
, Abolfazl Shirazi * , Mohsen Norouzian* , Ameneh Jafari , Haleh Edalatkhah , Maryam Mehravar , Mohammad Majidi , Mohammad Mehdi Mehrazar
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran & Department of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.
Abstract: (2560 Views)
Background: Currently, the efficient production of chimeric mice and their survival are still challenging. Recent researches have indicated that preimplantation embryo culture media and manipulation lead to abnormal methylation of histone in the H19/Igf2 promotor region and consequently alter their gene expression pattern. This investigation was designed to evaluate the relationship between the methylation state of histone
H3 and H19/Igf2 expression in mice chimeric blastocysts.
Methods: Mouse 129/Sv embryonic stem cells (mESCs) expressing the green fluorescent protein (mESCs- GFP) were injected into the perivitelline space of 2.5 days post-coitis (dpc) embryos (C57BL/6) using a micromanipulator. H3K4 and H3K9 methylation, and H19 and Igf2 expression was measured by immunocytochemistry and q-PCR, respectively, in blastocysts.
Results: Histone H3 trimethylation in H3K4 and H3K9 in chimeric blastocysts was significantly less and greater, respectively (p< 0.05), than in controls. H19 expression was significantly less (p< 0.05), while Igf2 expression was less, but not significantly so, in chimeric than in control blastocysts.
Conclusions: Our results showed, that the alteration ofH3K4me3 and H3K9me3 methylation, change H19/Igf2 expression in chimeric blastocysts.
H3 and H19/Igf2 expression in mice chimeric blastocysts.
Methods: Mouse 129/Sv embryonic stem cells (mESCs) expressing the green fluorescent protein (mESCs- GFP) were injected into the perivitelline space of 2.5 days post-coitis (dpc) embryos (C57BL/6) using a micromanipulator. H3K4 and H3K9 methylation, and H19 and Igf2 expression was measured by immunocytochemistry and q-PCR, respectively, in blastocysts.
Results: Histone H3 trimethylation in H3K4 and H3K9 in chimeric blastocysts was significantly less and greater, respectively (p< 0.05), than in controls. H19 expression was significantly less (p< 0.05), while Igf2 expression was less, but not significantly so, in chimeric than in control blastocysts.
Conclusions: Our results showed, that the alteration ofH3K4me3 and H3K9me3 methylation, change H19/Igf2 expression in chimeric blastocysts.
Type of Article: Original Article |
Subject:
Cell Biology
Received: 2020/07/9 | Accepted: 2020/07/19 | Published: 2020/12/1
Received: 2020/07/9 | Accepted: 2020/07/19 | Published: 2020/12/1
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