Reports of Biochemistry
and Molecular Biology
Sun, May 5, 2024
[Archive]
Volume 10, Issue 1 (Vol.10 No.1 Apr 2021)
rbmb.net 2021, 10(1): 30-40 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Abdelgawad L M, Mohy Abd El-hamed M, Sabry D, Abdelgwad M. Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Keap1/Nrf2/Ho-1 Pathway. rbmb.net 2021; 10 (1) :30-40
URL: http://rbmb.net/article-1-562-en.html
URL: http://rbmb.net/article-1-562-en.html
Department of Medical Laser applications, National Institute of laser enhanced science, Cairo University, Egypt.
Abstract: (3878 Views)
Background: Diabetes mellitus (DM) is a metabolic disorder resulting from hyperglycemia. Hyperglycemia contributes to oxidative stress, and the release of advanced glycation end products (AGEs) further promotes disease pathogenesis. Uncontrolled diabetes reflects great oral complications and affects human oral health. So, the present study aimed to assess the effects of photobiomodulation therapy (PBMT) and Metformin on proliferation and viability of human periodontal ligament stem cells (HPDLSCs) cultured in high glucose medium.
Methods: HPDLSCs were collected, isolated, and characterized and then divided into eight groups. Addition of extra glucose to diabetic groups 24 hours before cell irradiations. Metformin was added to half of the diabetic groups. Cells were irradiated with 808 nm diode laser 24, 48 hours. Cell viability was analyzed with MTT assay 24 hours post-irradiation to detect cell viability in each group. Real-time (PCR) was used to evaluate gene expression of Nrf2, Keap1, PIK3, and HO-1 and the effect of PBMT on Keap1/Nrf2/Ho-1 Pathway. ELISA reader was used to evaluating cell viability through (ROS, TNF-α, IL-10) protein levels after cell irradiation.
Results: Photobiomodulation at 1, 2, and 3 J/cm2 combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of Nrf2, Keap1, PIK3, and HO-1 levels (p< 0.05).
Conclusions: photobiomodulation with 3 J/cm2 combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.
Methods: HPDLSCs were collected, isolated, and characterized and then divided into eight groups. Addition of extra glucose to diabetic groups 24 hours before cell irradiations. Metformin was added to half of the diabetic groups. Cells were irradiated with 808 nm diode laser 24, 48 hours. Cell viability was analyzed with MTT assay 24 hours post-irradiation to detect cell viability in each group. Real-time (PCR) was used to evaluate gene expression of Nrf2, Keap1, PIK3, and HO-1 and the effect of PBMT on Keap1/Nrf2/Ho-1 Pathway. ELISA reader was used to evaluating cell viability through (ROS, TNF-α, IL-10) protein levels after cell irradiation.
Results: Photobiomodulation at 1, 2, and 3 J/cm2 combined with metformin significantly promoted diabetic cell lines of HPDLSCs viability (in MTT assay and ELISA reader of ROS, TNF-α, IL-10 results) and gene expression of Nrf2, Keap1, PIK3, and HO-1 levels (p< 0.05).
Conclusions: photobiomodulation with 3 J/cm2 combined with metformin enhanced proliferation and viability of diabetic cell lines of HPDLSCs and thus could improve differentiation and function of diabetic cell lines of HPDLSCs with minimum side effects.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2020/09/5 | Accepted: 2020/09/29 | Published: 2021/05/9
Received: 2020/09/5 | Accepted: 2020/09/29 | Published: 2021/05/9
References
1. Eliasson B, Cederholm J. The gap between guidelines and reality: Type 2 diabetes in a National Diabetes Register 1996-2003. Diabet Med. 2005;22(10):1420-6. [DOI:10.1111/j.1464-5491.2005.01648.x] [PMID]
2. American Diabetes Association. (2) Classification and diagnosis of diabetes. Diabetes Care. 2015; 38(Supplement 1):S8-S16. [DOI:10.2337/dc15-S005] [PMID]
3. Bryant W, Green field JR, Chisholm DJ, Campbell LV. Diabetes guidelines: easier to preach than to practice?. Med J Aust. 2006;185(6):305-9. [DOI:10.5694/j.1326-5377.2006.tb00583.x] [PMID]
4. Zhao GE, Hochwalt PC, Usui ML, Underwood RA, Singh PK, James GA, et al. Delayed wound healing in diabetic (db/db) mice with pseudomonas aeruginosa biofilm challenge: a model for the study of chronic wounds. Wound Repair Regen. 2010;18(5):467-77. [DOI:10.1111/j.1524-475X.2010.00608.x] [PMID] [PMCID]
5. Vernillo AT. Diabetes mellitus: relevance to dental treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endo. 2001;91(3):263-70. [DOI:10.1067/moe.2001.114002] [PMID]
6. Chavez EM, Borrell LN, Taylor GW, Ship JA. a longitudinal analysis of salivary flow in control subjects and older adults with type 2 diabetes. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;91(2):166-73. [DOI:10.1067/moe.2001.112054] [PMID]
7. Nwaneri C. Diabetes mellitus: a complete ancient and modern historical perspective. Webmedcentral. 2015.
8. Ginani F, Soares DM, Vasconcelos Barreto MPE, Barboza CAG. Effect of low level laser therapy on mesenchymal stem cell proliferation: a systematic review. Laser Med Sci. 2015;30(8):2189-94. [DOI:10.1007/s10103-015-1730-9] [PMID]
9. Zajac J, Shretha A, Patel P, Porestsky L. Principles of diabetes mellitus. Second Edition. Springer publishing; 2010.
10. Houreid N, Abrahams E H. in vitro exposure of wounded diabetic fibroblast cells to a helium-neon laser at 5 and 16 J/cm2. Photomed Laser surg. 2007;25(2):78-84. [DOI:10.1089/pho.2006.990] [PMID]
11. Verma SK, Maheshwari S, Kumar Singh R, Kumar Chaudhari P. Laser in dentistry: an innovative tool in modern dental practice. Natl J Maxillofac surg. 2012;3(2):124-32. [DOI:10.4103/0975-5950.111342] [PMID] [PMCID]
12. Oshima M, Mizuno M, Imamura A, Ogawa M, Yasukawa M, Yamazaki H, et al. Functional tooth regeneration using a bioengineered tooth unit as a mature organ replacement regenerative therapy. PLos One. 2011;6(7):e21531. [DOI:10.1371/journal.pone.0021531] [PMID] [PMCID]
13. Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T, et al. Fully functional bioengineered tooth replacement as an oragn replacement therapy. Proc Natl Acod SCi U S A. 2009;106(32):13475-80. [DOI:10.1073/pnas.0902944106] [PMID] [PMCID]
14. Kelly B, Tannabill GM, Murphy MP, O'Neill LAJ. Metformin inhibits the production of reactive oxygen species from NADH: ubiquinone oxidoreductase to limit induction of interleukin-1B(IL-1B) and boosts interleukin-10 (IL-10) in lipopolysaccharide (LPS)-activated macrophages. J Biol Chem. 2015;290(33):20348-59. [DOI:10.1074/jbc.M115.662114] [PMID] [PMCID]
15. Zorov DB, Juhaszova M, Solloll SJ. Mitochondrial Ros- induced ROS release: an update and review. Biochim Biophys Acta. 2006;1757(5-6):509-17. [DOI:10.1016/j.bbabio.2006.04.029] [PMID]
16. Konopka K, Goslinski T. Photodynamic Therapy in Dentistry. Journal of Dental Research. 2007;86(8):694-707. [DOI:10.1177/154405910708600803] [PMID]
17. Hong EG, Koh JH, cho YR, Kim HJ, Ma Z, Yu TY, et al. Interleukin-10 prevents diet- induced insulin resistance by attenuating macrophage and cytokine response in skeletal muscle. Diabetes. 2009;58(11):2525-35. [DOI:10.2337/db08-1261] [PMID] [PMCID]
18. Mainess MD, Mark JA, Ewing JF. Heme oxygenase, a likely regulator of cGMP production in the brain induction in vivo oh Ho-1 compensates for depression in NO synthase activity. Mol Cell Neurosci. 1993;4(5):398-405. [DOI:10.1006/mcne.1993.1050] [PMID]
19. Joussen AM, Poulaki V, Mitsiades N, Kirchhof B, Koizumi K, Döhmen S, et al. Nonsteroidal anti-inflammatory drugs prevent early diabetic retinopathy via TNF- alpha suppression. FASEB J. 2002;16(3):438-40. [DOI:10.1096/fj.01-0707fje] [PMID]
20. Ayuk SM, Houreld NN, Abrahamse H. Effect of 660 nm visible red light on cell proliferation and viability in diabetic models in vitro under stressed conditions. Lasers Med Sci. 2018;33(5):1085-1093 [DOI:10.1007/s10103-017-2432-2] [PMID]
21. Asghari M, Kanonisabet A, Safakhah M, Azimzadeh Z, Mostafavinia A, Taheri S, et al. the effect of combined photobiomodulation and metformin on open skin wound healing in a non-genetic model of type II diabetes. J Photochem Photobiol B. 2017;169:63-69. [DOI:10.1016/j.jphotobiol.2017.03.002] [PMID]
22. Vinck EM, Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC. Green light emitting diode irradiation enhances fibroblast growth impaired by high glucose level. Photomed laser surg. 2005;23(2):167-71. [DOI:10.1089/pho.2005.23.167] [PMID]
23. Hendudari F, Bayat M, Piryaei A. Combined effects of low-level laser therapy and human bone marrow mesenchymal stem cell conditioned medium on viability of human dermal fibroblasts cultured in a high-glucose medium. Laser med sci. 2016;31(4):749-57. [DOI:10.1007/s10103-016-1867-1] [PMID]
24. Kaspar JW, Niture SK, Jaisual AK. Nrf2:INrf2 (Keap1) signaling in oxidative stress. Free Radic Biol Med. 2009;47(9):1304-1309. [DOI:10.1016/j.freeradbiomed.2009.07.035] [PMID] [PMCID]
25. Long T, Liu G, Wang Y, Chen Y, Zhang Y, Qin. TNF-α, erectile dysfunction, and NADPH oxidase-mediated ROS generation in corpus cavernosum in high-fat diet/streptozotocin-induced diabetic rats. J Sex Med. 2012;9(7):1801-14. [DOI:10.1111/j.1743-6109.2012.02739.x] [PMID]
26. Chany Y. Quantitive data- parametric & Non-parametric tests. Singapore Med J, 2003; 44:391-396.
27. Liu Y, Yang H, Wen Y, Li B, Zhao Y, Xing J, et al. Nrf2 inhibits periodontal ligament stem cell apoptosis under excessive oxidative stress. Int J Mol Sci. 2017;18(5):1076. [DOI:10.3390/ijms18051076] [PMID] [PMCID]
28. Zare F, Bayat M, Aliaghaei A, Piryaei A. Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells. Laser in Med Sci. 2020;35(3):547-556. [DOI:10.1007/s10103-019-02844-y] [PMID]
29. Ke B, Shen XD, Zhargy Y, Haofeng Ji, Gao F, Yue S. KEAP1-NRF2 complex in ischemia- induced hepatic cellular damage of mouse liver transplants. J Hepatol. 2013;59(6):1200-1207. [DOI:10.1016/j.jhep.2013.07.016] [PMID] [PMCID]
30. Itoh K, Wakabayashi N, Katoh Y, Ishii T, O'Connor T, Yamamoto M. Keap1 regulates both cytoplasmic-nuclear shutting and degradation of Nrf2 in response to electrophiles. Genes cells. 2003;8(4):379-91. [DOI:10.1046/j.1365-2443.2003.00640.x] [PMID]
31. Yuan Z, Zhang J, Huang Y, Zhang Y, Liu W, Wang G, et al. NRF2 overexpression in mesenchymal stem cells induces stem-cell marker expression and enhances osteoblastic differentiation. Biochem Biophys Res Commun. 2017;491(1):228-235. [DOI:10.1016/j.bbrc.2017.07.083] [PMID]
32. Lee JS, Yi JK, An YS, Heo YS. increased osteogenic differentiation of periodontal ligament stem cells on polydopamine film occurs via activation of integrin and PI3K signaling pathways. Cell physiol biochem. 2014;34(5):1824-34. [DOI:10.1159/000366381] [PMID]
33. Chen H, Hung HS, Hsu ST. Low-energy laser irradiation increases endothelial gene expression possibly via PI3k signal pathway. Lasers surg Med.2008;40(1):46-54. [DOI:10.1002/lsm.20589] [PMID]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
