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Mirzaei B, Babaei R, Haghshenas M R, Hagshenas M R, Mohammadi F, Homayoni P et al . PIA and rSesC Mixture Arisen Antibodies Could Inhibit the Biofilm-Formation in Staphylococcus aureus. rbmb.net 2021; 10 (1) :1-12
URL: http://rbmb.net/article-1-574-en.html
URL: http://rbmb.net/article-1-574-en.html
PIA and rSesC Mixture Arisen Antibodies Could Inhibit the Biofilm-Formation in Staphylococcus aureus
Bahman Mirzaei * 
, Ryhaneh Babaei , Mohammad Reza Haghshenas , Mohammad Reza Hagshenas , Fatemeh Mohammadi , Pegah Homayoni , Ebrahim Shafaei
, Ryhaneh Babaei , Mohammad Reza Haghshenas , Mohammad Reza Hagshenas , Fatemeh Mohammadi , Pegah Homayoni , Ebrahim Shafaei
Department of Medical Microbiology and Virology, Faculty of Medicine, Mazandaran University of Medical Sciences. & Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Science.
Abstract: (3333 Views)
Background: Staphylococcus aureus as a causative agent of hospital-acquired infections has been considered as the primary concern in biomaterial-related infections (BAIs).
Methods: Following the purification of polysaccharide intercellular adhesion (PIA) as an efficient macromolecule in biofilm formation in the native condition, recombinant S. epidermidis surface-exposed rSesC protein, with the most homology to clumping factor A (ClfA) in S. aureus was cloned and expressed in a prokaryotic host as well. Fourier transform infrared spectrometry (FTIR) and Western blotting procedure analyzed purified PIA and protein, respectively. Then, the immune response was evaluated by measuring total IgG titers. Moreover, the capacity of Anti-biofilm forming activity of arisen antibodies to a biofilm-forming S. aureus strains was assessed by the semi-quantitative micro-plate procedure.
Results: Data showed that the total IgGs were boosted in mice immunized sera. By performing an inhibition assay, the biofilm inhibitory effect of secreted antibodies to test strain was observed. Arisen antibodies against the mixture significantly were more potent than PIA and rSesC, when comparing individual antigens in a biofilm inhibition assay.
Conclusions: immunization of mice with mentioned antigens especially a mixture of them, could eliminate the biofilm formation process in S. aureus. Hopefully, this study corresponds to the suggestion that the immunization of mice with PIA and rSesC candidate vaccines could protect against S. aureus infection.
Methods: Following the purification of polysaccharide intercellular adhesion (PIA) as an efficient macromolecule in biofilm formation in the native condition, recombinant S. epidermidis surface-exposed rSesC protein, with the most homology to clumping factor A (ClfA) in S. aureus was cloned and expressed in a prokaryotic host as well. Fourier transform infrared spectrometry (FTIR) and Western blotting procedure analyzed purified PIA and protein, respectively. Then, the immune response was evaluated by measuring total IgG titers. Moreover, the capacity of Anti-biofilm forming activity of arisen antibodies to a biofilm-forming S. aureus strains was assessed by the semi-quantitative micro-plate procedure.
Results: Data showed that the total IgGs were boosted in mice immunized sera. By performing an inhibition assay, the biofilm inhibitory effect of secreted antibodies to test strain was observed. Arisen antibodies against the mixture significantly were more potent than PIA and rSesC, when comparing individual antigens in a biofilm inhibition assay.
Conclusions: immunization of mice with mentioned antigens especially a mixture of them, could eliminate the biofilm formation process in S. aureus. Hopefully, this study corresponds to the suggestion that the immunization of mice with PIA and rSesC candidate vaccines could protect against S. aureus infection.
Type of Article: Original Article |
Subject:
Microbiology
Received: 2020/09/22 | Accepted: 2020/09/29 | Published: 2021/05/9
Received: 2020/09/22 | Accepted: 2020/09/29 | Published: 2021/05/9
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