Reports of Biochemistry
and Molecular Biology
Thu, Dec 26, 2024
[Archive]
Volume 9, Issue 4 (Vol.9 No.4 Jan 2021)
rbmb.net 2021, 9(4): 463-469 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Arfianti A, Sabillah Sumpena A, Andrini Djojosugito F, Kartika Sari D, Julia Paulina A. Genotype Distribution of Vitamin D Receptor Polymorphisms among Indonesian Patients with Chronic Hepatitis B. rbmb.net 2021; 9 (4) :463-469
URL: http://rbmb.net/article-1-603-en.html
URL: http://rbmb.net/article-1-603-en.html
Arfianti Arfianti * 
, Athalah Sabillah Sumpena , Fauzia Andrini Djojosugito , Dita Kartika Sari , Ariza Julia Paulina
, Athalah Sabillah Sumpena , Fauzia Andrini Djojosugito , Dita Kartika Sari , Ariza Julia Paulina
Department of Medical Biology, Faculty of Medicine, Universitas Riau, Pekanbaru, Indonesia.
Abstract: (3930 Views)
Background: Chronic hepatitis B is a necro-inflammatory of the liver parenchyma caused by hepatitis B virus (HBV) infection leading to liver cirrhosis and hepatocellular carcinoma (HCC). Genetic variants including single nucleotide polymorphisms (SNPs) within genes regulating immune response may contribute to the progression of chronic hepatitis B (CHB) infection. This study aimed to examine the genotype distribution of vitamin D receptor (VDR) polymorphism among patients with CHB infection and to study its association with the development of cirrhosis and hepatoma.
Methods: This cross-sectional study analysed 75 CHB patients, consisting of 36 CHB patients without cirrhosis, 25 CHB patients with cirrhosis, and 14 CHB patients with hepatoma. VDR polymorphism was examined using the Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) method.
Results: Alanine aminotransferase (ALT) and alpha fetoprotein (AFP) levels did not show any significant differences between study groups, but albumin levels in CHB patients with cirrhosis and hepatoma were significantly lower than CHB patients without cirrhosis (p< 0.05). In contrast, the bilirubin levels in CHB patients with cirrhosis was higher than in CHB patients’ cirrhosis. The most common genotypes of VDR polymorphisms were Ff (57.3%), TT (72%), aa (48%) and bb (74.7%) for Fok1, Taq1, Apa1 and Bsm1 respectively. There was no significant different in the genotype distribution of VDR polymorphism between CHB patients without cirrhosis and CHB with cirrhosis or hepatoma.
Conclusions: This study suggest that VDR gene polymorphism may not contribute to the progression of CHB infection.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2020/11/9 | Accepted: 2020/12/13 | Published: 2021/03/8
Received: 2020/11/9 | Accepted: 2020/12/13 | Published: 2021/03/8
References
1. World Health Organization. Global Hepatitis Report, 2017 [Internet]. WHO. 2017. 62 p. Available from: https://apps.who.int/iris/handle/10665/255016
2. H Muljono D. Epidemiology of Hepatitis B and C in Republic of Indonesia. Euroasian J Hepatogastroenterol. 2017;7(1):55-59. [DOI:10.5005/jp-journals-10018-1212] [PMID] [PMCID]
3. Zhang Z, Wang C, Liu Z, Zou G, Li J, Lu M. Host Genetic Determinants of Hepatitis B Virus Infection. Front Genet. 2019;10:696. [DOI:10.3389/fgene.2019.00696] [PMID] [PMCID]
4. Zhang J, Liu L, Lin Z, Ji X, Pi L, Lin X, et al. SNP-SNP and SNP-environment interactions of potentially functional HOTAIR SNPs modify the risk of hepatocellular carcinoma. Mol Carcinog. 2019;58(5):633-642. [DOI:10.1002/mc.22955] [PMID]
5. Trepo C, Chan HL, Lok A. Hepatitis B virus infection. Lancet. 2014;384(9959):2053-63. [DOI:10.1016/S0140-6736(14)60220-8]
6. Meng S, He S, Jiang W, Xiao L, Li D, Xu J, et al. Genetic susceptibility to autoimmune thyroid diseases in a Chinese Han population: Role of vitamin D receptor gene polymorphisms. Ann Endocrinol (Paris). 2015;76(6):684-9. [DOI:10.1016/j.ando.2015.01.003] [PMID]
7. Meng Z, Chen Y, Lu M. Advances in Targeting the Innate and Adaptive Immune Systems to Cure Chronic Hepatitis B Virus Infection. Front Immunol. 2019;10:3127. [DOI:10.3389/fimmu.2019.03127] [PMID] [PMCID]
8. Yang PL, Althage A, Chung J, Maier H, Wieland S, Isogawa M, et al. Immune effectors required for hepatitis B virus clearance. Proc Natl Acad Sci U S A. 2010;107(2):798-802. [DOI:10.1073/pnas.0913498107] [PMID] [PMCID]
9. Bizzaro G, Antico A, Fortunato A, Bizzaro N. Vitamin D and Autoimmune Diseases: Is Vitamin D Receptor (VDR) Polymorphism the Culprit?. Isr Med Assoc J. 2017;19(7):438-443.
10. Mondul AM, Weinstein SJ, Layne TM, Albanes D. Vitamin D and Cancer Risk and Mortality: State of the Science, Gaps, and Challenges. Epidemiol Rev. 2017;39(1):28-48. [DOI:10.1093/epirev/mxx005] [PMID] [PMCID]
11. Zhai N, Bidares R, Makoui MH, Aslani S, Mohammadi P, Razi B, et al. Vitamin D receptor gene polymorphisms and the risk of the type 1 diabetes: a meta-regression and updated meta-analysis. BMC Endocr Disord. 2020;20:121. [DOI:10.1186/s12902-020-00575-8] [PMID] [PMCID]
12. Farnik H, Bojunga J, Berger A, Allwinn R, Waidmann O, Kronenberger B, et al. Low vitamin D serum concentration is associated with high levels of hepatitis B virus replication in chronically infected patients. Hepatology. 2013;58(4):1270-6. [DOI:10.1002/hep.26488] [PMID]
13. Gao W, Wang R, Wang X, Wu H, Wang Y, Lu X, et al. Vitamin D serum levels and receptor genetic polymorphisms are associated with hepatitis B virus and HIV infections and IFN-λ levels. Biomark Med. 2017;11(9):733-740. [DOI:10.2217/bmm-2017-0022] [PMID]
14. Sassi F, Tamone C, D'amelio P. Vitamin D: Nutrient, hormone, and immunomodulator. Nutrients. 2018;10(11):1656. [DOI:10.3390/nu10111656] [PMID] [PMCID]
15. Tizaoui K, Hamzaoui K. Association between VDR polymorphisms and rheumatoid arthritis disease: Systematic review and updated meta-analysis of case-control studies. Immunobiology. 2015;220(6):807-16. [DOI:10.1016/j.imbio.2014.12.013] [PMID]
16. Salimi S, Farajian-Mashhadi F, Alavi-Naini R, Talebian G, Narooie-Nejad M. Association between vitamin D receptor polymorphisms and haplotypes with pulmonary tuberculosis. Biomed Rep. 2015;3(2):189-194. [DOI:10.3892/br.2014.402] [PMID] [PMCID]
17. Gandini S, Gnagnarella P, Serrano D, Pasquali E, Raimondi S. Vitamin D receptor polymorphisms and cancer. Adv Exp Med Biol. 2014;810:69-105. [DOI:10.1007/978-1-4939-0437-2_5] [PMID]
18. Jafari M, Pirouzi A, Anoosheh S, Farnia P, Tajik N. Rapid and simultaneous detection of vitamin D receptor gene polymorphisms by a single ARMS-PCR assay. Mol Diagn Ther. 2014;18(1):97-103. [DOI:10.1007/s40291-013-0060-5] [PMID]
19. Setiabudiawan B, Kartasasmita CB, Garna H, Parwati I, Maskoen AM. Polimorfisme FokI, BsmI, ApaI, dan TaqI Gen Reseptor Vitamin D pada Kejadian Tuberkulosis Anak. MKB. 2010;42:187. [DOI:10.15395/mkb.v42n4.35]
20. Hoan NX, Khuyen N, Giang DP, Binh MT, Toan NL, Anh DT, et al. Vitamin D receptor ApaI polymorphism associated with progression of liver disease in Vietnamese patients chronically infected with hepatitis B virus. BMC Medical Genetics. 2019;20:201. [DOI:10.1186/s12881-019-0903-y] [PMID] [PMCID]
21. Ramos-Lopez E, Kurylowicz A, Bednarczuk T, Paunkovic J, Seidl C, Badenhoop K. Vitamin D receptor polymorphisms are associated with Graves' disease in German and Polish but not in Serbian patients. Thyroid. 2005;15(10):1125-30. 22. Mohammed MA, Shabana T, Sheta NM, Omar SAM. Vitamin D Receptor Gene Polymorphisms as a Predictive Risk Factor for Hepatocellular Carcinoma Development and Severity in Chronic Hepatitis B. International Journal of Cancer Research. 2017;13(1):26-35. [DOI:10.1089/thy.2005.15.1125] [PMID]
22. Yao X, Zeng H, Zhang G, Zhou W, Yan Q, Dai L, et al. The associated ion between the VDR gene polymorphisms and susceptibility to hepatocellular carcinoma and the clinicopathological features in subjects infected with HBV. Biomed Res Int. 2013;2013:953974. [DOI:10.1155/2013/953974] [PMID] [PMCID]
23. Mostafa-Hedeab G, Sabry D, Mostafa Abdelaziz G, Ewaiss M, Adli N, Fathy W. Influence of Vitamin D Receptor Gene Polymorphisms on Response to Pegylated Interferon in Chronic Hepatitis B Egyptian Patients. Rep Biochem Mol Biol. 2018;6(2):186-196.
24. He Q, Huang Y, Zhang L, Yan Y, Liu J, Song X, et al. Association between vitamin D receptor polymorphisms and hepatitis B virus infection susceptibility: A meta-analysis study. Gene. 2018;645:105-12. [DOI:10.1016/j.gene.2017.12.027] [PMID]
25. Tangoh D, Mahmood Y, Apinjoh T, Nyingchu R, Azhar A, Achidi E. A Rapid Multiplex ARMS-PCR Method for the Detection of Four Single Nucleotide Polymorphisms of the Vitamin D Receptor (VDR) Gene. Imp J Interdiscip Res. 2017;3(9):573-80.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
