Volume 3, Issue 2 (Vol.3 No.2 Apr 2015)                   rbmb.net 2015, 3(2): 76-81 | Back to browse issues page

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Mahmoudi Rad M, Mahmoudi Rad N, Mirdamadi Y. Expression of TGF-β3 in Isolated Fibroblasts from Foreskin. rbmb.net. 2015; 3 (2) :76-81
URL: http://rbmb.net/article-1-62-en.html
Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran - Department of Dermatology and Venereology, Otto-von Guericke University Magdeburg, Magdeburg, Germany
Abstract:   (4375 Views)

Background: The multifunctional transforming growth factor beta (TGF-β) is a glycoprotein that exists in three isoforms. TGF-β3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. Fibroblasts are key cells in the wound healing process. TGF-β3 plays a critical role in scar-free wound healing and fibroblast actions in the wound healing process. The aim of this study was to express the TGF-β3 gene (tgf-b3) in human foreskin fibroblasts (HFF’s).

Methods: We obtained HFF’s from a newborn and a primary fibroblast culture was prepared. The cells were transfected with TGF-β3-pCMV6-XL5 plasmid DNA by both lipofection and electroporation. Expression of TGF-β3 was measured by enzyme-linked immunosorbent assay (ELISA).

Results: The highest TGF-β3 expression (8.3-fold greater than control) was obtained by lipofection after 72 hours using 3 µl of transfection reagent. Expression was 1.4-fold greater than control by electroporation.

Conclusions: In this study, we successfully increased TGF-β3 expression in primary fibroblast cells. In the future, grafting these transfected fibroblasts onto wounds can help the healing process without scarring.

Full-Text [PDF 359 kb]   (1547 Downloads)    
Subject: Cell Biology
Received: 2014/11/15 | Accepted: 2014/12/12 | Published: 2015/04/30

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