Volume 11, Issue 4 (Vol.11 No.4 Jan 2023)                   rbmb.net 2023, 11(4): 730-738 | Back to browse issues page


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Mohamed Abdelgawad L, Abdullatif Abdelaziz A, Bawdy El-Begawey M, Mohamed Saafan A. Influence of Nanocurcumin and Photodynamic Therapy Using Nanocurcumin in Treatment of Rat Tongue Oral Squamous Cell Carcinoma Through Histological Examination and Gene Expression of BCL2 and Caspase-3. rbmb.net 2023; 11 (4) :730-738
URL: http://rbmb.net/article-1-845-en.html
Department of Medical Applications of Lasers, National Institute of Laser Enhanced Sciences, Cairo University, Cairo, Egypt.
Abstract:   (1693 Views)
Background: Oral squamous cell carcinoma (OSCC) is the sixth most common mouth cancer in the world. The aim of the present study is comparing the effects of using Nanocurcumin, and photodynamic therapy (PDT), alone or together in treatment of OSCC in rats.

Methods: Forty Wister male rats were divided into Control (group 1), 650 nm diode Laser only (group 2), Nanocurcumin alone (group 3), and PDT with a combination of laser with Nanocurcumin (group 4). Then, OSCC in the tongue induced by dimethylbenz anthracene (DMBA). The treatments were evaluated clinically, histopathologically, and immunohistochemically through BCL2 and Caspase-3 genes expression.

Results: Positive control with OSCC displayed significant weight loss, while PDT group gained more than nanocurcumin treated groups as well as laser groups comparing with control positive group.
The histological examination of the tongue in PDT group showed improvement. In laser group, there were partial loss of surface epithelium with various ulcers and dysplasia and partial improvement by this type of treatment. The tongue in the positive control group showed ulcer in the dorsum surface with inflammatory cells, hyperplasia of the mucosa membrane around the ulcer (acanthosis) with increase of dentition, vacuolar degeneration of prickle cell layer and increase mitotic activity of basal cell layer together with dermal proliferation.

Conclusions: Under the condition of the present study, PDT using nanocurcumin photosensitizer was effective in the treatment of OSCC regarding clinical, histological and gene expression of BCL2 and Caspase-3.
Full-Text [PDF 278 kb]   (1077 Downloads)    
Type of Article: Original Article | Subject: Molecular Biology
Received: 2021/12/27 | Accepted: 2021/12/29 | Published: 2023/04/3

References
1. Giovannacci I, Vescovi P, Manfredi M, Meleti M. Non-invasive visual tools for diagnosis of oral cancer and dysplasia: A systematic review. Med Oral Patol Oral Cir Bucal. 2016;21(3):e305-15. [DOI:10.4317/medoral.20996] [PMID] [PMCID]
2. Maleki D, Ghojazadeh M, Mahmoudi SS, Mahmoudi SM, Pournaghi-Azar F, Torab A, et al. Epidemiology of Oral Cancer in Iran: a Systematic Review. Asian Pac J Cancer Prev. 2015;16(13):5427-32. [DOI:10.7314/APJCP.2015.16.13.5427] [PMID]
3. Mascitti M, Orsini G, Tosco V, Monterubbianesi R, Balercia A, Putignano A, et al. An Overview on Current Non-invasive Diagnostic Devices in Oral Oncology. Front Physiol. 2018;9:1510. [DOI:10.3389/fphys.2018.01510] [PMID] [PMCID]
4. Givony Sh. Oral squamous cell carcinoma (OSCC) an overview. J Med Scie.2020;8(13):67-74.
5. El-Haddad, K., Metwaly, H. Evaluation of dose dependent effects of gamma radiation on rat tongue internal structures: histomorphometric and immunohistochemical study. Egyptian Dental Journal. 2018;64 (2):1315-1325. [DOI:10.21608/edj.2018.77388]
6. Almangush A, Levio I, Mäkitie AA. Biomarkers for Immunotherapy of Oral Squamous Cell Carcinoma: Current Status and Challenges. Front. Oncol. 2021;11:1-5. [DOI:10.3389/fonc.2021.616629] [PMID] [PMCID]
7. Carreras-Torras C, Gay-Escoda C. Techniques for early diagnosis of oral squamous cell carcinoma: Systematic review. Med Oral Patol Oral Cir Bucal. 2015;20(3):e305-15. [DOI:10.4317/medoral.20347] [PMID] [PMCID]
8. Alhusaini A, Fadda L, Hasan IH, Zakaria E, Alenazi AM, Mahmoud AM. Curcumin Ameliorates Lead-Induced Hepatotoxicity by Suppressing Oxidative Stress and Inflammation, and Modulating Akt/GSK-3β Signaling Pathway. Biomolecules. 2019;9(11):703. [DOI:10.3390/biom9110703] [PMID] [PMCID]
9. Bakhshi M, Gholami S, Mahboubi A, Jaafari MR, Namdari M. Combination Therapy with 1% Nanocurcumin Gel and 0.1% Triamcinolone Acetonide Mouth Rinse for Oral Lichen Planus: A Randomized Double-Blind Placebo Controlled Clinical Trial. Dermatol Res Pract.2020;2020:4298193. [DOI:10.1155/2020/4298193] [PMID] [PMCID]
10. Zhang XD, Wu D, Shen X, Liu PX, Fan FY, Fan SJ. In vivo renal clearance, biodistribution, toxicity of gold nanoclusters. Biomaterials. 2012;33(18):4628-38. [DOI:10.1016/j.biomaterials.2012.03.020] [PMID]
11. Singh DV, Godbole MM, Misra K. A plausible explanation for enhanced bioavailability of P-gp substrates in presence of piperine: simulation for next generation of P-gp inhibitors. J Mol Model. 2013;19(1):227-38. [DOI:10.1007/s00894-012-1535-8] [PMID]
12. Schiborr C, Kocher A, Behnam D, Jandasek J, Toelstede S, Frank J. The oral bioavailability of curcumin from micronized powder and liquid micelles is significantly increased in healthy humans and differs between sexes. Mol Nutr Food Res. 2014;58(3):516-27. [DOI:10.1002/mnfr.201300724] [PMID]
13. Buss S, Dobra J, Goerg K, Hoffmann S, Kippenberger S, Kaufmann R, et al. Visible light is a better co-inducer of apoptosis for curcumin-treated human melanoma cells than UVA. PLoS One. 2013;8(11):e79748. [DOI:10.1371/journal.pone.0079748] [PMID] [PMCID]
14. Liew YCC, De Souza NNA, Sultana RG, Oh CC. Photodynamic therapy for the prevention and treatment of actinic keratosis/squamous cell carcinoma in solid organ transplant recipients: a systematic review and meta-analysis. J Eur Acad Dermatol Venereol. 2020;34(2):251-259. [DOI:10.1111/jdv.15852] [PMID]
15. Lin J, Ni G, Ding T, Lei S, Zhong L, Liu N, et al. Photodynamic Therapy for Oral Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis. Int J of Photoenerg. 2021;6641358:1-14. [DOI:10.1155/2021/6641358]
16. Hamblin MR. Mechanisms and applications of the anti-inflammatory effects of photobiomodulation. AIMS Biophys. 2017;4(3):337-361. [DOI:10.3934/biophy.2017.3.337] [PMID] [PMCID]
17. The Lancet (EDITORIAL). Stem cells, regenerative medicine, Prometheus. Editorial. Lancet. 2018;391(10123):814. [DOI:10.1016/S0140-6736(18)30548-8] [PMID]
18. de Pauli Paglioni M, Faria KM, Palmier NR, Prado-Ribeiro AC, E Dias RB, da Graça Pinto H, et al. Patterns of oral mucositis in advanced oral squamous cell carcinoma patients managed with prophylactic photobiomodulation therapy-insights for future protocol development. Lasers Med Sci. 2021;36(2):429-436. [DOI:10.1007/s10103-020-03091-2] [PMID]
19. Chen X, Duan N, Zhang C, Zhang W. Survivin and Tumorigenesis: Molecular Mechanisms and Therapeutic Strategies. J Cancer. 2016;7(3):314-23. [DOI:10.7150/jca.13332] [PMID] [PMCID]
20. Ailioaie LM, Litscher G. Curcumin and Photobiomodulation in Chronic Viral Hepatitis and Hepatocellular Carcinoma. Int J Mol Sci. 2020;21(19):7150. [DOI:10.3390/ijms21197150] [PMID] [PMCID]
21. Beyer K, Nikfarjam F, Butting M, Meissner M, König A, Ramirez Bosca A, et al. Photodynamic Treatment of Oral Squamous Cell Carcinoma Cells with Low Curcumin Concentrations. J Cancer. 2017;8(7):1271-1283. [DOI:10.7150/jca.17176] [PMID] [PMCID]
22. Zhang C, Li B, Zhang X, Hazarika P, Aggarwal BB, Duvic M. Curcumin selectively induces apoptosis in cutaneous T-cell lymphoma cell lines and patients' PBMCs: potential role for STAT-3 and NF-kappaB signaling. J Invest Dermatol. 2010;130(8):2110-9. [DOI:10.1038/jid.2010.86] [PMID]
23. Faustino-Rocha A, Oliveira PA, Pinho-Oliveira J, Teixeira-Guedes C, Soares-Maia R, da Costa RG, et al. Estimation of rat mammary tumor volume using caliper and ultrasonography measurements. Lab Anim (NY). 2013;42(6):217-24. [DOI:10.1038/laban.254] [PMID]
24. Juneja S, Chaitanya NB, Agarwal M. Immunohistochemical expression of Bcl-2 in oral epithelial dysplasia and oral squamous cell carcinoma. Indian J Cancer. 2015;52(4):505-10. [DOI:10.4103/0019-509X.178411] [PMID]
25. Latteri S, Catania VE, Malaguarnera G, Peri A, Bertino G, Frazzetto G, et al. Carcino embryonic Antigen Serum Levels in Nonmelanoma Skin Cancer. Biomedicines.2018;6(24):1-11. [DOI:10.3390/biomedicines6010024] [PMID] [PMCID]
26. Ramezani M, Mohamadzaheri E, Khazaei S, Najafi F, Vaisi-Raygani A, Rahbar M, Sadeghi M. Comparison of EMA, CEA, CD10 and Bcl-2 Biomarkers by Immunohistochemistry in Squamous Cell Carcinoma and Basal Cell Carcinoma of the Skin. Asian Pac J Cancer Prev. 2016;17(3):1379-83. [DOI:10.7314/APJCP.2016.17.3.1379] [PMID]
27. Scuderi SA, Casili G, Basilotta R, Lanza M, Filippone A, Raciti G, et al. NLRP3 Inflammasome Inhibitor BAY-117082 Reduces Oral Squamous Cell Carcinoma Progression. Int J Mol Sci. 2021;22(20):11108. [DOI:10.3390/ijms222011108] [PMID] [PMCID]
28. Wang J, Zhang X, Wei P, Zhang J, Niu Y, Kang N, et al. Livin, Survivin and Caspase 3 as early recurrence markers in non-muscle-invasive bladder cancer. World J Urol. 2014;32(6):1477-84. [DOI:10.1007/s00345-014-1246-0] [PMID]
29. Kangboonruang K, Wongtrakoongate P, Lertsuwan K, Khachonkham S, Changkaew P, Tangboonduangjit P. et al. MALAT1 Decreases the Sensitivity of Head and Neck Squamous Cell Carcinoma Cells to Radiation and Cisplatin Anticancer Res. 2020;40(5):2645-55. [DOI:10.21873/anticanres.14235] [PMID]
30. Ahn J, Kang J, Shin J, Chung P. Combination treatment with photodynamic therapy and curcumin induces mitochondria-dependent apoptosis in AMC-HN3 cells. Int J of Oncology. 2012;41(6),2184-2190. [DOI:10.3892/ijo.2012.1661] [PMID]
31. Chen CH, Huang YC, Lee YH, Tan ZL, Tsai CJ, Chuang YC, et al. Anticancer Efficacy and Mechanism of Amentoflavone for Sensitizing Oral Squamous Cell Carcinoma to Cisplatin. Anticancer Res. 2020;40(12):6723-6732. [DOI:10.21873/anticanres.14695] [PMID]
32. Prasad S, Tyagi AK, Aggarwal BB. Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat. 2014;46(1):2-18. [DOI:10.4143/crt.2014.46.1.2] [PMID] [PMCID]
33. Liu T, Long T, Li H. Curcumin suppresses the proliferation of oral squamous cell carcinoma through a specificity protein 1/nuclear factor-κB-dependent pathway. Exp Ther Med. 2021;21(3):202. [DOI:10.3892/etm.2021.9635] [PMID] [PMCID]
34. Ludwig LM, Nassin ML, Hadji A, LaBelle JL. Killing Two Cells with One Stone: Pharmacologic BCL-2 Family Targeting for Cancer Cell Death and Immune Modulation. Front Pediatr. 2016;4:135. [DOI:10.3389/fped.2016.00135] [PMID] [PMCID]
35. Jakubowska K, Guzińska-Ustymowicz K, Famulski W, Cepowicz D, Jagodzińska D, Pryczynicz A. Reduced expression of caspase-8 and cleaved caspase-3 in pancreatic ductal adenocarcinoma cells. Oncol Lett. 2016;11(3):1879-1884. [DOI:10.3892/ol.2016.4125] [PMID] [PMCID]
36. Noble P, Vyas M, Al-Attar A, Durrant S, Scholefield J, Durrant L. High levels of cleaved caspase-3 in colorectal tumour stroma predict good survival. Br J Cancer. 2013;108(10):2097-105. [DOI:10.1038/bjc.2013.166] [PMID] [PMCID]
37. Coutinho-Camillo CM, Lourenço SV, Nishimoto IN, Kowalski LP, Soares FA. Caspase expression in oral squamous cell carcinoma. Head Neck. 2011;33(8):1191-8. [DOI:10.1002/hed.21602] [PMID]
38. Huang Q, Li F, Liu X, Li W, Shi W, Liu FF, et al. Caspase 3- mediated stimulation of tumor cell repopulation during cancer radiotherapy. Nat Med. 2011;17(7):860-6. [DOI:10.1038/nm.2385] [PMID] [PMCID]
39. Ryoo HD, Bergmann A. The role of apoptosis-induced proliferation for regeneration and cancer. Cold Spring Harb Perspect Biol. 2012;4(8):a008797. [DOI:10.1101/cshperspect.a008797] [PMID] [PMCID]
40. Hassan M, Watari H, AbuAlmaaty A, Ohba Y, Sakuragi N. Apoptosis and molecular targeting therapy in cancer. Biomed Res Int. 2014;2014:150845. [DOI:10.1155/2014/150845] [PMID] [PMCID]
41. Li S, Zhang HY, Wang T, Meng X, Zong ZH, Kong DH, et al. BAG3 promoted starvation-induced apoptosis of thyroid cancer cells via attenuation of autophagy. J Clin Endocrinol Metab. 2014;99(11):E2298-307. [DOI:10.1210/jc.2014-1779] [PMID]
42. Mohamed Abdelgawad L, Abd El-Hamed MM, Sabry D, Abdelgwad M. Efficacy of Photobiomodulation and Metformin on Diabetic Cell Line of Human Periodontal Ligament Stem Cells through Keap1/Nrf2/Ho-1 Pathway. Rep Biochem Mol Biol. 2021;10(1):30-40. [DOI:10.52547/rbmb.10.1.30] [PMID] [PMCID]
43. Shahabinejad M, Zare R, Asadi Z, Mohajertehran F. LAMP3 (CD208) Expression in Squamous Cell Carcinoma and Epithelial Dysplasia of the Oral Cavity and Clinicopathological Characteristics of Unfavorable Prognosis. Rep Biochem Mol Biol. 2021;9(4):373-378. [DOI:10.52547/rbmb.9.4.373] [PMCID]
44. Abdelgwad M, Sabry D, Mohamed Abdelgawad L, Mohamed Elroby Ali D. In Vitro Differential Sensitivity of Head and Neck Squamous Cell Carcinoma to Cisplatin, Silver Nanoparticles, and Photodynamic Therapy. Rep Biochem Mol Biol. 2022;11(2):224-237.

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