Reports of Biochemistry
and Molecular Biology
Tue, Oct 15, 2024
[Archive]
Volume 11, Issue 2 (Vol.11 No.2 Jul 2022)
rbmb.net 2022, 11(2): 320-326 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Dewi S, Triatmono V R, Rasyada Ralas P R, Veraldi V, Alfian I, Iswanti F C et al . Increasing of LDH Specific Activity and PEPCK Level Play a Role on Activation of Gluconeogenesis Pathway in Early Onset
Pre-Eeclampsia Placenta. rbmb.net 2022; 11 (2) :320-326
URL: http://rbmb.net/article-1-884-en.html
URL: http://rbmb.net/article-1-884-en.html
Syarifah Dewi 
, Visabella Rizky Triatmono , Puti Raykhan Rasyada Ralas , Veraldi Veraldi , Irvan Alfian , Febriana Catur Iswanti , Ani Retno Prijanti *
, Visabella Rizky Triatmono , Puti Raykhan Rasyada Ralas , Veraldi Veraldi , Irvan Alfian , Febriana Catur Iswanti , Ani Retno Prijanti *
1: Department of Biochemistry and Molecular Biology, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia & Center of Hypoxia and Oxidative Stress Studies, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia.
Abstract: (2201 Views)
Background: Recent advancement on experiment concluded that etiology of pre-eclampsia (PE) could be explained by the "two-stage" theory. The theory of which explained that pre-eclampsia occurs due to abnormalities in spiral arteries development and release of inflammatory response. Failure of spiral arteries development, the lesion of damage could be due to ischemia-reperfusion or hypoxia-reoxygenation. Hypoxia in pre-eclamptic placenta leads to metabolic change to anaerobic in glycolysis. Lactate dehydrogenase (LDH) has important role in anaerobic glycolysis that catalyzes the conversion of lactate to pyruvate during hypoxia. On the other hand, phosphoenolpyruvate carboxy kinase (PEPCK) is merely an enzyme of gluconeogenesis. This research conduct to reveal that in early onset pre-eclampsia the placenta still hypoxic and undergoes gluconeogenesis even after delivery, through metabolic enzyme of LDH and PEPCK level.
Methods: This cross-sectional study compared early onset PE (< 34 weeks) with normal term placenta. We measured LDH enzyme activity using colorimetric assay and PEPCK protein using ELISA method.
Results: Result show that placental LDH specific activity was increased significantly in PE with median 2.750 (0.030 - 5.680) U/mg compared to normal term placenta 0.255 (0.032 – 1.194) U/mg (Mann-Whitney, p< 0.001). PEPCK level was significantly increased in PE 8.998 (1.737-44.914) ng/mg compared to normal term placenta 1.552 (0.741-8.832) ng/mg (Mann-Whitney, p< 0.001).
Conclusions: We conclude that anaerobic glycolysis and gluconeogenesis pathway are increased in early onset PE placenta as adaptation mechanism to hypoxic condition.
Methods: This cross-sectional study compared early onset PE (< 34 weeks) with normal term placenta. We measured LDH enzyme activity using colorimetric assay and PEPCK protein using ELISA method.
Results: Result show that placental LDH specific activity was increased significantly in PE with median 2.750 (0.030 - 5.680) U/mg compared to normal term placenta 0.255 (0.032 – 1.194) U/mg (Mann-Whitney, p< 0.001). PEPCK level was significantly increased in PE 8.998 (1.737-44.914) ng/mg compared to normal term placenta 1.552 (0.741-8.832) ng/mg (Mann-Whitney, p< 0.001).
Conclusions: We conclude that anaerobic glycolysis and gluconeogenesis pathway are increased in early onset PE placenta as adaptation mechanism to hypoxic condition.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2022/02/18 | Accepted: 2022/02/23 | Published: 2022/08/7
Received: 2022/02/18 | Accepted: 2022/02/23 | Published: 2022/08/7
References
1. Uzan J, Carbonnel M, Piconne O, Asmar R, Ayoubi J-M. Pre-eclampsia: pathophysiology, diagnosis, and management. Vasc Health Risk Manag. 2011;7:467-474. [DOI:10.2147/VHRM.S20181] [PMID] [PMCID]
2. Sammour MB, el-Kabarity H, Fawzy MM, Schindler AE. Prevention and treatment of pregnancy-induced hypertension (preeclampsia) with progestogens. J Steroid Biochem Mol Biol. 2005;97(5):439-40. [DOI:10.1016/j.jsbmb.2005.08.014] [PMID]
3. Kahnamoueiaghdam F, Amani F, Hamidimoghaddam S. Prevalence of pre-eclampsia and eclampsia risk factors among pregnant women, 2011-2013. International Journal of Advances in Medicine. 2015;2(2):128-132. [DOI:10.5455/2349-3933.ijam20150510]
4. Gathiram P, Moodley J. Pre-eclampsia: its pathogenesis and pathophysiolgy. Cardiovascular Journal of Africa. 2016;27(2):71-8. [DOI:10.5830/CVJA-2016-009] [PMID] [PMCID]
5. Mayrink J, Costa ML, Cecatti JG. Preeclampsia in 2018: Revisiting Concepts, Physiopathology, and Prediction. Scientific WorldJournal. 2018;2018:6268276. [DOI:10.1155/2018/6268276] [PMID] [PMCID]
6. Zhao L, Ma R, Zhang L, Yuan X, Wu J, He L, et al. Inhibition of HIF-1a-mediated TLR4 activation decreases apoptosis and promotes angiogenesis of placental microvascular endothelial cells during severe pre-eclampsia pathogenesis. Placenta. 2019;83:8-16. [DOI:10.1016/j.placenta.2019.06.375] [PMID]
7. Mary VP, Chellatamizh M, Padmanaban S. Role of serum LDH in preeclampsia as a prognostic factor - a cross sectional case control study in tertiary care hospital. Int J Reprod Contraception, Obstet Gynecol. 2017;6(2):595. [DOI:10.18203/2320-1770.ijrcog20170389]
8. Ababio G, Adu-Bonsaffoh K, Narh G, Morvey D, Botchway F, Abindau E, et al. Effects of Lactate Dehydrogenase (LDH) in Preeclampsia. Clinical & Medical Biochemistry. 2017;03(2):1-6. [DOI:10.4172/2471-2663.1000129]
9. Bender DA, Mayes PA. Metabolism of Carbohydrates. Harper's Illustrated Biochemistry. 2015.
10. Franko KL, Forhead AJ, Fowden AL. Effects of stress during pregnancy on hepatic glucogenic capacity in rat dams and their fetuses. Physiol Rep. 2017;5(11):e13293. [DOI:10.14814/phy2.13293] [PMID] [PMCID]
11. Labarrere CA, DiCarlo HL, Bammerlin E, Hardin JW, Kim YM, Chaemsaithong P, et al. Failure of physiologic transformation of spiral arteries, endothelial and trophoblast cell activation, and acute atherosis in the basal plate of the placenta. Am J Obstet Gynecol. 2017;216(3):287.e1-287.e16. [DOI:10.1016/j.ajog.2016.12.029] [PMID] [PMCID]
12. Prijanti AR, Marissa N, Paramita R, Humaira S, Nabila EN, Wijaya AE, et al. Analysis of oxidative stress markers malondialdehyde, glutathione, nitric oxide, and prorenin level in preeclampsia placental tissues. Asian J Pharm Clin Res. 2018;11(1):158-61.
https://doi.org/10.22159/ajpcr.2017.v11i1.18330 [DOI:10.22159/ajpcr.2018.v11i1.18330]
13. Ridwan R, Iswanti FC, Sadikin M. The increased of Carbonic anhydrase in liver tissue of rat induced by chronic systemic hypoxia. Acta Biochimica Indonesiana. 2018;1(1). [DOI:10.32889/actabioina.v1i1.1]
14. Prijanti AR, Indarwati T, Mudjihartini N, Yulhasri, Sadikin M. Performance of Gluconeogenesis in Rat Kidneys Due to Systemic Hypoxia. Adv Sci Lett. 2018;24(9):6781-6784. [DOI:10.1166/asl.2018.12840]
15. Marissa N, Jusman SWA, Purwosunu Y, Prijanti AR. Correlation of (PRO)renin receptor and vascular endothelial growth factor expression level in third trimester of preeclampsia placentas. Asian J Pharm Clin Res. 2018;11(12):1-5. [DOI:10.22159/ajpcr.2018.v11i12.23173]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
