Volume 11, Issue 3 (Vol.11 No.3 Oct 2022)                   rbmb.net 2022, 11(3): 405-410 | Back to browse issues page


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Movahhed T, Mehrabkhani M, Arefnezhad M, HAmedi S S, Zare Mahmoudabadi R, Ghanbari F et al . Antibacterial Efficacy of Walnut Green Husk (WGH) Extract with Zinc Oxide Nanoparticles on Streptococcus Mutans. rbmb.net 2022; 11 (3) :405-410
URL: http://rbmb.net/article-1-909-en.html
Dental School, Mashhad University of Medical Sciences, Iran.
Abstract:   (347 Views)
Background: Chemical agents, such as Chlorhexidine are used as one of dental plaque control strategy. Researchers are looking for a natural and economic substitute with same antibacterial efficacy and less complications. The aim of this study was to evaluate the antimicrobial efficacy of the Khorasan Razavi walnut green husk extract with and without adding ZnO nanoparticles (nZnO) on  (S. mutans).

Methods: In this in vitro study, antimicrobial effect of the Hydro-ethanolic extract of WGH, was evaluated. Broth Dilution and Agar diffusion methods were used with 90 tubes containing different dilutions of WGH extract (100 to 0.006 mg/ml). ZnO nanoparticles (nZnO) were added to 45 tubes. Streptococcus mutans was exposed to 15 different serial concentrations of study extracts, from 100 mg/ml to 0.006 mg/ml. Minimum inhibitory concentration (MIC) of the study extracts were determined and zone of inhibition diameter was compared to positive controls (chlorhexidine 0.2%, nZnO), and negative control (sterile distilled water). The differences between the mean diameters, were analyzed by independent sample T- teS.

Results: Minimum inhibitory concentration (MIC) of study extract was found to be 50 mg/mL, with adding nZnO, MIC was reduced to 3.12 mg/mL. Mean diameter of inhibition zone at 3.12 mg/ml with and without adding ZnO nanoparticles were 17.67±0.57 mm and 8±0.001 mm, respectively, (p-value< 0.001).

Conclusions: Adding nZnO could be enhanced antimicrobial efficacy of the WGH extract against S. mutants, while it was still less effective than chlorhexidine.
Full-Text [PDF 245 kb]   (132 Downloads)    
Type of Article: Original Article | Subject: Microbiology
Received: 2022/04/8 | Accepted: 2022/04/8 | Published: 2022/12/31

References
1. Youssefi MA, Afroughi S. Prevalence and Associated Factors of Dental Caries in Primary Schoolchildren: An Iranian Setting. International Journal of Dentistry. 2020;(18):1-7. [DOI:10.1155/2020/8731486] [PMID] [PMCID]
2. Caglar E, Kargul B, Tanboga I. Bacteriotherapy and probiotics' role on oral health. Oral Dis. 2005;11(3):131-7. [DOI:10.1111/j.1601-0825.2005.01109.x] [PMID]
3. Mazzari AL, Prieto JM. Herbal medicines in Brazil: pharmacokinetic profile and potential herb-drug interactions. Front in Pharmacol. 2014;5:162. [DOI:10.3389/fphar.2014.00162] [PMID] [PMCID]
4. Oliveira I, Sousa A, Valentão P, Andrade PB, Ferreira IC, Ferreres F, et al. Hazel (Corylus avellana L.) leaves as source of antimicrobial and antioxidative compounds. Food chemistry. 2007;105(3):1018-1025. [DOI:10.1016/j.foodchem.2007.04.059]
5. Vahdati K, Massah Bavani AR, Khosh-Khui M, Fakour P, Sarikhani S. Applying the AOGCM-AR5 models to the assessments of land suitability for walnut cultivation in response to climate change: a case study of Iran. PLoS One. 2019;14(6):e0218725. [DOI:10.1371/journal.pone.0218725] [PMID] [PMCID]
6. Mahoney N, Molyneux RJ, Campbell BC. Regulation of aflatoxin production by naphthoquinones of walnut (Juglans regia). J Agric Food Chem. 2000;48(9):4418-21. [DOI:10.1021/jf0003449] [PMID]
7. Rahimipanah M, Hamedi M, Mirzapour M. Analysis of some factors affecting the phenolic compounds extracted from green husk of walnut (Juglans regia L.). Iranian Journal of Medicinal and Aromatic Plants Research. 2011;27(3):419-430.
8. Oliveira I, Sousa A, Ferreira IC, Bento A, Estevinho L, Pereira JA. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food Chem Toxicol. 2008;46(7):2326-31. [DOI:10.1016/j.fct.2008.03.017] [PMID]
9. Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev. 1998;56(11):317-33. [DOI:10.1111/j.1753-4887.1998.tb01670.x] [PMID]
10. Coppo E, Marchese A. Antibacterial activity of polyphenols. Curr Pharm Biotechnol. 2014;15(4):380-90. [DOI:10.2174/138920101504140825121142] [PMID]
11. Pitts G, Brogdon C, Hu L, Masurat T, Pianotti R, Schumann P. Mechanism of action of an antiseptic, anti-odor mouthwash. J Dent Res. 1983;62(6):738-42. [DOI:10.1177/00220345830620061001] [PMID]
12. Buzea C, Pacheco II, Robbie K. Nanomaterials and nanoparticles: sources and toxicity. Biointerphases. 2007;2(4):MR17-71. [DOI:10.1116/1.2815690] [PMID]
13. Król A, Pomastowski P, Rafińska K, Railean-Plugaru V, Buszewski B. Zinc oxide nanoparticles: Synthesis, antiseptic activity and toxicity mechanism. Adv Colloid Interface Sci. 2017;249:37-52. [DOI:10.1016/j.cis.2017.07.033] [PMID]
14. Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols. 2008;3(2):163-175. [DOI:10.1038/nprot.2007.521] [PMID]
15. Sheydaei P, Bayrami A, Azizian Y, Parvinroo S. Study on the toxicity effects of zinc oxide nanoparticles on hematological and serum parameters in mice. Journal of Arak University of Medical Sciences. 2017;19(10):39-47.
16. Sharafatichaleshtori R, Sharafatichaleshtori F, Sharafatichaleshtori A, Rafieian M. Antibacterial effects of ethanolic extract of walnut leaves (Juglans regia) on propionibacterium acnes. Journal of Zanjan University of Medical Sciences and Health Services.. 2010;18(71):42-49.
17. Arji P, Naseri A, Rakhshandeh H, Najafzadeh MJ. Investigation of antifungal activity of methanol and aqueous extracts of walnut (Juglans regia) leaves and peel against candida species. Journal of Birjand University of Medical Sciences. 2015;22(2):115-24.
18. Chaleshtori RS, Chaleshtori FS, Rafieian M. Biological characterization of Iranian walnut (Juglans regia) leaves. Turkish Journal of Biology. 2011;35(5):635-3639. [DOI:10.3906/biy-1005-1]
19. Zakavi F, Golpasand Hagh L, Daraeighadikolaei A, Farajzadeh Sheikh A, Daraeighadikolaei A, Leilavi Shooshtari Z. Antibacterial effect of Juglans regia bark against oral pathologic bacteria. International journal of dentistry. 2013;2013. [DOI:10.1155/2013/854765] [PMID] [PMCID]
20. Darmani H, Nusayr T, Al‐Hiyasat A. Effects of extracts of miswak and derum on proliferation of Balb/C 3T3 fibroblasts and viability of cariogenic bacteria. Int J Hyg. 2006;4(2):62-6. [DOI:10.1111/j.1601-5037.2006.00149.x] [PMID]
21. Deshpande RR, Kale AA, Ruikar AD, Panvalkar PS, Kulkarni AA, Deshpande NR, et al. Antimicrobial activity of different extracts of Juglans regia L. against oral microflora. Int J Pharm Pharm Sci. 2011;3(2):200-201.
22. Nam K-Y. In vitro antimicrobial effect of the tissue conditioner containing silver nanoparticles. J Adv Prosthodont. 2011;3(1):20-24. [DOI:10.4047/jap.2011.3.1.20] [PMID] [PMCID]
23. Jung WK, Koo HC, Kim KW, Shin S, Kim SH, Park YH. Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Appl Environ Microbiol. 2008;74(7):2171-8. [DOI:10.1128/AEM.02001-07] [PMID] [PMCID]

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