Volume 11, Issue 4 (Vol.11 No.4 Jan 2023)                   rbmb.net 2023, 11(4): 547-552 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zare R, Abdolsamadi H, Soleimani Asl S, Radi S, Bahrami H, Jamshidi S. The bFGF Can Improve Angiogenesis in Oral Mucosa and Accelerate Wound Healing. rbmb.net 2023; 11 (4) :547-552
URL: http://rbmb.net/article-1-926-en.html
Department of Oral and Maxillofacial Pathology, School of Dentistry, Dental Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Abstract:   (1612 Views)
Background: The role of the basic fibroblast growth factor (bFGF) has well known in the angiogenesis and ulcer healing. In this study, we aimed to evaluate the effects of bFGF on tissue repair in a rat oral mucosal wound.

Methods: Musosal wound induced on the lip mucosa of rats and bFGF was injected along the edge of the mucosal defect immediately after surgery. The tissues were collected on days 3, 7 and 14 after the wound induction. The micro vessel density (MVD) and CD34 expression were done by histochemical studies.

Results: The bFGF significantly accelerated granulation tissue formation and MVD was increased three days after ulcer induction but decreased 14 days after surgery. The MVD was significantly higher in the bFGF-treated group. The wound area was decreased in all groups time-dependently and a statistically significant difference (p value?) was observed between the bFGF-treated group and untreated group. The wound area was smaller in the bFGF-treated group compared to the untreated group.

Conclusions: Our data demonstrated that bFGF can accelerated and facilitated wound healing.
Full-Text [PDF 235 kb]   (1003 Downloads)    
Type of Article: Original Article | Subject: Cell Biology
Received: 2022/04/25 | Accepted: 2022/05/3 | Published: 2023/04/3

References
1. Muñoz-Corcuera M, Esparza-Gómez G, González-Moles MA, Bascones-Martínez A. Oral ulcers: clinical aspects. A tool for dermatologists. Part I. Acute ulcers. Clin Exp Dermatol. 2009;34(3):289-94. [DOI:10.1111/j.1365-2230.2009.03220.x] [PMID]
2. Ernawati DS, Surboyo MDC, Ayuningtyas NF, Nagoro AAB. Role of Inflammatory Cell Responses in Stimulating Fibroblasts in Diabetic Oral Ulcer after Treatment with Liquid Smoke of Coconut Endocarp: A Histological Assessment. Eur J Dent. 2021;15(1):71-76. [DOI:10.1055/s-0040-1715913] [PMID] [PMCID]
3. Yu Z, Jin C, Xin M, JianMin H. Effect of Aloe vera polysaccharides on immunity and antioxidant activities in oral ulcer animal models. Carbohydrate Polymers. 2009;75(2):307-11. [DOI:10.1016/j.carbpol.2008.07.029]
4. Woodley DT, O'Keefe EJ, Prunieras M. Cutaneous wound healing: a model for cell-matrix interactions. J Am Acad Dermatol. 1985;12(2 Pt 2):420-33. [DOI:10.1016/S0190-9622(85)80005-0] [PMID]
5. Boilly B, Vercoutter-Edouart AS, Hondermarck H, Nurcombe V, Le Bourhis X. FGF signals for cell proliferation and migration through different pathways. Cytokine Growth Factor Rev. 2000;11(4):295-302. [DOI:10.1016/S1359-6101(00)00014-9] [PMID]
6. Presta M, Andrés G, Leali D, Dell'Era P, Ronca R. Inflammatory cells and chemokines sustain FGF2-induced angiogenesis. Eur Cytokine Netw. 2009;20(2):39-50. [DOI:10.1684/ecn.2009.0155] [PMID]
7. Zhu X-J, Liu Y, Dai Z-M, Zhang X, Yang X, Li Y, et al. BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin. PLoS Genet. 2014;10(10):e1004687. [DOI:10.1371/journal.pgen.1004687] [PMID] [PMCID]
8. Shimabukuro Y, Ichikawa T, Takayama S, Yamada S, Takedachi M, Terakura M, et al. Fibroblast growth factor-2 regulates the synthesis of hyaluronan by human periodontal ligament cells. J Cell Physiol. 2005;203(3):557-63. [DOI:10.1002/jcp.20256] [PMID]
9. Oda Y, Kagami H, Ueda M. Accelerating effects of basic fibroblast growth factor on wound healing of rat palatal mucosa. J Oral Maxillofac Surg. 2004;62(1):73-80. [DOI:10.1016/j.joms.2003.05.007] [PMID]
10. Fox SB, Harris AL. Markers of tumor angiogenesis: clinical applications in prognosis and anti-angiogenic therapy. Invest New Drugs.1997;15(1):15-28. [DOI:10.1023/A:1005714527315] [PMID]
11. Dantas Filho AM, Aguiar JLdA, Rocha LsRdM, Azevedo ÃtM, Ramalho E, Medeiros AC. Effects of the basic fibroblast growth factor and its anti-factor in the healing and collagen maturation of infected skin wound. Acta Cir Bras. 2007;22:64-71. [DOI:10.1590/S0102-86502007000700013] [PMID]
12. Kumar V, Abbas Abul K, Fausto N, Mitchell R. Robbins Basic Pathology. 8th Edition, Saunders Elsevier. Philadelphia; 2010.
13. Matsumoto S, Tanaka R, Okada K, Arita K, Hyakusoku H, Miyamoto M, et al. The effect of control-released basic fibroblast growth factor in wound healing: histological analyses and clinical application. Plast Reconstr Surg Glob Open. 2013;1(6):e44. [DOI:10.1097/GOX.0b013e3182a88787] [PMID] [PMCID]
14. Kibe Y, Takenaka H, Kishimoto S. Spatial and temporal expression of basic fibroblast growth factor protein during wound healing of rat skin. Br J Dermatol. 2000;143(4):720-7. [DOI:10.1046/j.1365-2133.2000.03824.x] [PMID]
15. Broadley KN, Aquino AM, Hicks B, Ditesheim JA, McGee GS, Demetriou AA, et al. Growth factors bFGF and TGB beta accelerate the rate of wound repair in normal and in diabetic rats. Int J Tissue React.1988;10(6):345-53.
16. Chakrabarti S, Mazumder B, Rajkonwar J, Pathak MP, Patowary P, Chattopadhyay P. bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway. Sci Rep. 2021;11(1):3357. [DOI:10.1038/s41598-021-82888-9] [PMID] [PMCID]
17. Hata Y, Kawanabe H, Hisanaga Y, Taniguchi K, Ishikawa H. Effects of basic fibroblast growth factor administration on vascular changes in wound healing of rat palates. Cleft Palate Craniofac J. 2008;45(1):63-72. [DOI:10.1597/06-166.1] [PMID]
18. Uhl E, Barker JH, Bondàr I, Galla TJ, Leiderer R, Lehr HA, Messmer K. Basic fibroblast growth factor accelerates wound healing in chronically ischaemic tissue. Br J Surg. 1993;80(8):977-80. [DOI:10.1002/bjs.1800800812] [PMID]
19. Fu X, Sheng Z, Wang Y, Ye Y, Xu M, Sun T, et al. Basic fibroblast growth factor reduces the gut and liver morphologic and functional injuries after ischemia and reperfusion. J Trauma. 1997;42(6):1080-5. [DOI:10.1097/00005373-199706000-00016] [PMID]
20. Mustoe TA, Pierce GF, Morishima C, Deuel TF. Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model. J Clin Invest. 1991;87(2):694-703. [DOI:10.1172/JCI115048] [PMID] [PMCID]
21. Ohshima M, Sato M, Ishikawa M, Maeno M, Otsuka K. Physiologic levels of epidermal growth factor in saliva stimulate cell migration of an oral epithelial cell line, HO-1-N-1. Eur J Oral Sci. 2002;110(2):130-6. [DOI:10.1034/j.1600-0722.2002.11179.x] [PMID]
22. Takayama S, Murakami S, Miki Y, Ikezawa K, Tasaka S, Terashima A, et al. Effects of basic fibroblast growth factor on human periodontal ligament cells. J Periodontal Res. 1997;32(8):667-75. [DOI:10.1111/j.1600-0765.1997.tb00577.x] [PMID]
23. Murakami S, Takayama S, Ikezawa K, Sltimabukuro Y, Kitamura M, Nozaki T, et al. Regeneration of periodontal tissues by basic fibroblast growth factor. J Periodontal Res. 1999;34(7):425-30. [DOI:10.1111/j.1600-0765.1999.tb02277.x] [PMID]
24. Van Setten GB. Basic fibroblast growth factor in human saliva: detection and physiological implications. Laryngoscope. 1995;105(6):610-2. [DOI:10.1288/00005537-199506000-00009] [PMID]
25. Stenberg BD, Phillips LG, Hokanson JA, Heggers JP, Robson MC. Effect of bFGF on the inhibition of contraction caused by bacteria. J Surg Res. 1991;50(1):47-50. [DOI:10.1016/0022-4804(91)90008-A] [PMID]
26. Arundina I, Diyatri I, Surboyo MDC, Monica E, Afanda NM. Growth factor stimulation for the healing of traumatic ulcers with liquid rice hull smoke. J Taibah Univ Med Sci. 2021;16(3):431-439. [DOI:10.1016/j.jtumed.2021.01.003] [PMID] [PMCID]
27. Kottakis F, Polytarchou C, Foltopoulou P, Sanidas I, Kampranis SC, Tsichlis PN. FGF-2 regulates cell proliferation, migration, and angiogenesis through an NDY1/KDM2B-miR-101-EZH2 pathway. Mol Cell. 2011;43(2):285-98. [DOI:10.1016/j.molcel.2011.06.020] [PMID] [PMCID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2015 All Rights Reserved | Reports of Biochemistry and Molecular Biology

Designed & Developed by : Yektaweb