Volume 11, Issue 3 (Vol.11 No.3 Oct 2022)                   rbmb.net 2022, 11(3): 457-464 | Back to browse issues page


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amin R, Shariff M A, Purwanita P, Saleh M I. Efficacy of Sambiloto Extracts, Andrographis paniculate, (Burm. F) in Inhibiting Diabetic Retinopathy Progression: An in Vivo Study. rbmb.net 2022; 11 (3) :457-464
URL: http://rbmb.net/article-1-946-en.html
Department of Ophthalmology, Faculty of Medicine, Universitas Sriwijaya/Dr Moh Hoesin General Hospital, Palembang, Indonesia.
Abstract:   (333 Views)
Background: Diabetic retinopathy (DR) is one of diabetes mellitus complication and occurred in retinal microvascular. This study was aimed to investigate the efficacy of Sambiloto, Andrographis paniculate (A. paniculata) extract on glycemic profile, antioxidant and inflammatory cytokine parameters in diabetic rats, and phytochemical analysis of A. paniculata.

Methods: A. paniculata extract (APE) was carried out by maceration with ethanol. Diabetes mellitus in Wistar male rats was induced with streptozotocin. Retinal vessel diameters were estimated using a method by Vucetic. Inflammatory cytokine and antioxidant parameters were evaluated in retinal tissue. The alkaloid and flavonoid contents in extract were analyzed using thin layer chromatography method.

Results: Funduscopic examination presented some changes in the diameter of the blood vessels. The vessel diameter in the diabetic retinopathy group with APE in concentration of 100 and 200 mg/kg BW groups was significantly lower than in the DR group (p<0.05). The administration of APE in dosages of 100 and 200 mg/kg BW showed reduced glutathione, SOD, and catalase levels compared to the DR group (p<0.05).

Conclusions: A. paniculata extract doses of 100 and 200 mg/kg BW improved diabetic retinopathy in rats through hypoglycemic effects, antioxidant effects, and anti-inflammatory mechanisms.
Full-Text [PDF 284 kb]   (124 Downloads)    
Type of Article: Original Article | Subject: Molecular Biology
Received: 2022/05/15 | Accepted: 2022/05/21 | Published: 2022/12/31

References
1. Mansour SE, Browning DJ, Wong K, Flynn HW Jr, Bhavsar AR. The Evolving Treatment of Diabetic Retinopathy. Clin Ophthalmol. 2020;14:653-678. [DOI:10.2147/OPTH.S236637] [PMID] [PMCID]
2. Endris T, Worede A, Asmelash D. Prevalence of Diabetes Mellitus, Prediabetes and Its Associated Factors in Dessie Town, Northeast Ethiopia: A Community-Based Study. Diabetes Metab Syndr Obes. 2019;12:2799-2809. [DOI:10.2147/DMSO.S225854] [PMID] [PMCID]
3. Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J. 2016;24(5):547-553. [DOI:10.1016/j.jsps.2015.03.013] [PMID] [PMCID]
4. Semeraro F, Morescalchi F, Cancarini A, Russo A, Rezzola S, Costagliola C. Diabetic retinopathy, a vascular and inflammatory disease: Therapeutic implications. Diabetes Metab. 2019;45(6):517-527. [DOI:10.1016/j.diabet.2019.04.002] [PMID]
5. Shaito A, Thuan DTB, Phu HT, Nguyen THD, Hasan H, Halabi S, Abdelhady S, Nasrallah GK, Eid AH, Pintus G. Herbal Medicine for Cardiovascular Diseases: Efficacy, Mechanisms, and Safety. Front Pharmacol. 2020;11:422. [DOI:10.3389/fphar.2020.00422] [PMID] [PMCID]
6. Hidayat R, Wulandari P. Effects of Andrographis paniculata (Burm. F.) extract on diabetic nephropathy in rats. Rep Biochem Mol Bio. 2021;10(3):445-54. [DOI:10.52547/rbmb.10.3.445] [PMID] [PMCID]
7. Wediasari F, Nugroho GA, Fadhilah Z, Elya B, Setiawan H, Mozef T. Hypoglicemic effect of a combined Andrographis paniculata and Caesalpinia sappan extract in streptozocin-induced diabetic rats. Adv Pharmacol Pharm Sci. 2020;8856129. [DOI:10.1155/2020/8856129] [PMID] [PMCID]
8. Rocházková D, Bousová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 2011;82(4):513-23. [DOI:10.1016/j.fitote.2011.01.018] [PMID]
9. Gheibi S, Kashfi K, Ghasemi A. A practical guide for induction of type-2 diabetes in rat: incorporating a high-fat diet and streptozotocin. Biomed Pharmacother. 2017;95:605-13. [DOI:10.1016/j.biopha.2017.08.098] [PMID]
10. Vucetic M, Jensen PK, Jansen EC. Diameter variations of retinal blood vessels during and after treatment with hyperbaric oxygen. Br J Opthalmol. 2004;88:771-5. [DOI:10.1136/bjo.2003.018788] [PMID] [PMCID]
11. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione-S-transferase activities in lung and liver. Biochem Biophys Acta. 1979;582(1): 67-78. [DOI:10.1016/0304-4165(79)90289-7] [PMID]
12. Misra HP, Fridovich I. The oxidation of phenylhydrazine: superoxide and mechanism. Biochemistry. 1976;15:681-7. [DOI:10.1021/bi00648a036] [PMID]
13. Aebi H. Catalase. In: Bergmeyer HU, ed. Methods in enzymatic analysis. 1st ed. New York. Academic Press, 1974:673-7. [DOI:10.1016/B978-0-12-091302-2.50032-3]
14. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin-phenol reagent. J Biol Chem. 1951;193:265-75. [DOI:10.1016/S0021-9258(19)52451-6] [PMID]
15. Andrade-Cetto A, Rubalcaba Mares ML. Hypoglycemic effect of the Rhizophora mangle Cortex on STZ-NA induced diabetic rats. Pharmacol Online. 2012;3(3):1-5.
16. National Research Council (US) Institute for Laboratory Animal Research. Guide for the Care and Use of Laboratory Animals. Washington (DC): National Academies Press (US);1996.
17. Bek T. Diameter Changes of Retinal Vessels in Diabetic Retinopathy. Curr Diab Rep. 2017;17(10):82. [DOI:10.1007/s11892-017-0909-9] [PMID]
18. Papatheodorou K, Banach M, Bekiari E, Rizzo M, Edmonds M. Complications of Diabetes 2017. J Diabetes Res. 2018;2018:3086167. [DOI:10.1155/2018/3086167] [PMID] [PMCID]
19. Klein R, Myers CE, Lee KE, Gangnon R, Klein BE. Changes in retinal vessel diameter and incidence and progression of diabetic retinopathy. Arch Ophthalmol. 2012;130(6):749-55. [DOI:10.1001/archophthalmol.2011.2560] [PMID] [PMCID]
20. Gui F, You Z, Fu S, Wu H, Zhang Y. Endothelial Dysfunction in Diabetic Retinopathy. Front Endocrinol (Lausanne). 2020;11:591. [DOI:10.3389/fendo.2020.00591] [PMID] [PMCID]
21. Kaštelan S, Orešković I, Bišćan F, Kaštelan H, Gverović Antunica A. Inflammatory and angiogenic biomarkers in diabetic retinopathy. Biochem Med (Zagreb). 2020;30(3):030502. [DOI:10.11613/BM.2020.030502] [PMID] [PMCID]
22. Behl Y, Krothapalli P, Desta T, DiPiazza A, Roy S, Graves DT. Diabetes-enhanced tumor necrosis factor- production promotes apoptosis and the loss of retinal microvascular cells in type 1 and type 2 models of diabetic retinopathy. Am J Pathol. 2008;172(5):1411-8. [DOI:10.2353/ajpath.2008.071070] [PMID] [PMCID]
23. Zafar MI, Mills K, Ye X, Blakely B, Min J, Kong W, et al. Association between the expression of vascular endothelial growth factors and metabolic syndrome or its components: a systematic review and meta-analysis. Diabetology Metab Syndrome. 2018;10:62. [DOI:10.1186/s13098-018-0363-0] [PMID] [PMCID]
24. Semadi IN. The role of VEGF and TNF-alpha on epithelialization of diabetic foot ulcers after hyperbaric oxygen therapy. Open Access Maced J Med Sci. 2019;7(19):3177-83. [DOI:10.3889/oamjms.2019.297] [PMID] [PMCID]
25. Balbi ME, Tonn FS, Mendes AM, Borba HH, Wiens A, Fernandez-Llimos F, et al. Antioxidant effects of vitamins in type 2 diabetes: a meta-analysis of randomized controlled trials. Diabetol Metab Syndr. 2018;10:18. [DOI:10.1186/s13098-018-0318-5] [PMID] [PMCID]
26. Albert-Garay JS, Riesgo-Escovar JR, Salceda R. High glucose concentrations induce oxidative stress by inhibiting Nrf2 expression in rat Müller retinal cells in vitro. Sci Rep. 2022;12(1):1261. [DOI:10.1038/s41598-022-05284-x] [PMID] [PMCID]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2015 All Rights Reserved | Reports of Biochemistry and Molecular Biology

Designed & Developed by : Yektaweb