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Volume 11, Issue 4 (Vol.11 No.4 Jan 2023)
rbmb.net 2023, 11(4): 577-589 |
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Proskurina A, Nikolin V, Popova N, Varaksin N, Ryabicheva T, Ershova E, et al . Comparing the Biological Properties of Double-Stranded DNA Extracted from Human and Porcine Placenta and Salmon Sperm. rbmb.net 2023; 11 (4) :577-589
URL: http://rbmb.net/article-1-1014-en.html
URL: http://rbmb.net/article-1-1014-en.html
Anastasia Proskurina 
, Valeriy Nikolin , Nelly Popova , Nikolay Varaksin , Tatiana Ryabicheva , Elizaveta Ershova , Svetlana Kostyuk , Olga Leplina , Alexandr Ostanin , Elena Chernykh , Sergey Bogachev *
, Valeriy Nikolin , Nelly Popova , Nikolay Varaksin , Tatiana Ryabicheva , Elizaveta Ershova , Svetlana Kostyuk , Olga Leplina , Alexandr Ostanin , Elena Chernykh , Sergey Bogachev *
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Abstract: (2064 Views)
Background: Double-stranded fragmented extracellular DNA is a participant, inducer, and indicator of various processes occurring in the organism. When investigating the properties of extracellular DNA, the question regarding the specificity of exposure to DNA from different sources has always been raised. The aim of this study was to perform comparative assessment of biological properties of double-stranded DNA obtained from the human placenta, porcine placenta and salmon sperm.
Methods: The intensity of leukocyte-stimulating effect of different dsDNA was assessed in mice after cyclophosphamide-induced cytoreduction. The stimulatory effect of different dsDNA on maturation and functions of human dendritic cells and the intensity of cytokine production by human whole blood cells was analyzed ex vivo. The oxidation level of the dsDNA was also compared.
Results: Human placental DNA exhibited the strongest leukocyte-stimulating effect. DNA extracted from human and porcine placenta exhibited similar stimulatory action on maturation of dendritic cells, allostimulatory capacity, and ability of dendritic cells to induce generation of cytotoxic CD8+CD107a+ T cells in the mixed leukocyte reaction. DNA extracted from salmon sperm stimulated the maturation of dendritic cells, while having no effect on their allostimulatory capacity. DNA extracted from human and porcine placenta was shown to exhibit a stimulatory effect on cytokine secretion by human whole blood cells. The observed differences between the DNA preparations can be caused by the total methylation level and are not related to differences in oxidation level of DNA molecules.
Conclusions: Human placental DNA exhibited the maximum combination of all biological effects.
Methods: The intensity of leukocyte-stimulating effect of different dsDNA was assessed in mice after cyclophosphamide-induced cytoreduction. The stimulatory effect of different dsDNA on maturation and functions of human dendritic cells and the intensity of cytokine production by human whole blood cells was analyzed ex vivo. The oxidation level of the dsDNA was also compared.
Results: Human placental DNA exhibited the strongest leukocyte-stimulating effect. DNA extracted from human and porcine placenta exhibited similar stimulatory action on maturation of dendritic cells, allostimulatory capacity, and ability of dendritic cells to induce generation of cytotoxic CD8+CD107a+ T cells in the mixed leukocyte reaction. DNA extracted from salmon sperm stimulated the maturation of dendritic cells, while having no effect on their allostimulatory capacity. DNA extracted from human and porcine placenta was shown to exhibit a stimulatory effect on cytokine secretion by human whole blood cells. The observed differences between the DNA preparations can be caused by the total methylation level and are not related to differences in oxidation level of DNA molecules.
Conclusions: Human placental DNA exhibited the maximum combination of all biological effects.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2022/07/30 | Accepted: 2022/10/9 | Published: 2023/04/3
Received: 2022/07/30 | Accepted: 2022/10/9 | Published: 2023/04/3
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