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Volume 11, Issue 4 (Vol.11 No.4 Jan 2023)
rbmb.net 2023, 11(4): 644-655 |
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Jafar Sameri M, Belali R, Neisi N, Noei Razliqi * R, Mard S A, Savari F et al . Sodium Hydrosulfide Modification of Mesenchymal Stem Cell-Exosomes Improves Liver Function in CCL4-Induced Hepatic Injury in Mice. rbmb.net 2023; 11 (4) :644-655
URL: http://rbmb.net/article-1-1070-en.html
URL: http://rbmb.net/article-1-1070-en.html
Maryam Jafar Sameri * 
, Rafie Belali , Niloofar Neisi , Reza Noei Razliqi * , Seyed Ali Mard , Feryal Savari , Seyyed Saeed Azandeh
, Rafie Belali , Niloofar Neisi , Reza Noei Razliqi * , Seyed Ali Mard , Feryal Savari , Seyyed Saeed Azandeh
Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran & Physiology department, Abadan University of Medical Sciences, Abadan, Iran.
Abstract: (2265 Views)
Background: Liver diseases and injuries are important medical problems worldwide. Acute liver failure (ALF) is a clinical syndrome characterized by severe functional impairment and widespread death of hepatocytes. Liver transplantation is the only treatment available so far. Exosomes are nanovesicles originating from intracellular organelles. They regulate the cellular and molecular mechanisms of their recipient cells and have promising potential for clinical application in acute and chronic liver injuries. This study compares the effect of Sodium hydrosulfide (NaHS) modified exosomes with non-modified exosomes in CCL4-induced acute liver injury to ascertain their role in ameliorating hepatic injury.
Methods: Human Mesenchymal stem cells (MSCs) were treated with or without NaHS (1 μmol) and exosomes were isolated using an exosome isolation kit. Male mice (8-12 weeks old) were randomly divided into four groups (n=6): 1-control, 2-PBS, 3- MSC-Exo, and 4- H2S-Exo. Animals received 2.8 ml/kg body weight of CCL4 solution intraperitoneally, and 24 h later MSC-Exo (non-modified), H2S-Exo (NaHS-modified), or PBS, was injected in the tail vein. Moreover, 24 h after Exo administration, mice were sacrificed for tissue and blood collection.
Results: Administration of both MSC-Exo and H2S-Exo reduced inflammatory cytokines (IL-6, TNF-α), total oxidant levels, liver aminotransferases, and cellular apoptosis.
Conclusions: MSC-Exo and H2S-Exo had hepato-protective effects against CCL4-induced liver injury in mice. Modification of cell culture medium with NaHS as an H2S donor enhances the therapeutic effects of MSC exosomes.
Methods: Human Mesenchymal stem cells (MSCs) were treated with or without NaHS (1 μmol) and exosomes were isolated using an exosome isolation kit. Male mice (8-12 weeks old) were randomly divided into four groups (n=6): 1-control, 2-PBS, 3- MSC-Exo, and 4- H2S-Exo. Animals received 2.8 ml/kg body weight of CCL4 solution intraperitoneally, and 24 h later MSC-Exo (non-modified), H2S-Exo (NaHS-modified), or PBS, was injected in the tail vein. Moreover, 24 h after Exo administration, mice were sacrificed for tissue and blood collection.
Results: Administration of both MSC-Exo and H2S-Exo reduced inflammatory cytokines (IL-6, TNF-α), total oxidant levels, liver aminotransferases, and cellular apoptosis.
Conclusions: MSC-Exo and H2S-Exo had hepato-protective effects against CCL4-induced liver injury in mice. Modification of cell culture medium with NaHS as an H2S donor enhances the therapeutic effects of MSC exosomes.
Keywords: Acute liver failure (ALF), CCL4-induced liver injury, Exosomes, Mesenchymal stem cells (MSCs), Sodium hydrosulfide (NaHS).
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2022/10/19 | Accepted: 2022/12/1 | Published: 2023/04/3
Received: 2022/10/19 | Accepted: 2022/12/1 | Published: 2023/04/3
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