Volume 11, Issue 3 (Vol.11 No.3 Oct 2022)                   rbmb.net 2022, 11(3): 493-501 | Back to browse issues page


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Khadir F, Rahimi Z, Vaisi-raygani A, shakiba E, Naseri R. Gestational Diabetes Mellitus (GDM), Hypothyroidism, and Gene Variants (Keap1 Rs11085735) in Patients with Preeclampsia. rbmb.net 2022; 11 (3) :493-501
URL: http://rbmb.net/article-1-1082-en.html
Department of Clinical Biochemistry, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran & Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Abstract:   (361 Views)
Background: Preeclampsia is a multifactorial hypertensive disorder of pregnancy with multisystem involvement. Recent studies have demonstrated that preeclampsia is associated with increased placental oxidative stress at the cellular level. The nuclear factor erythroid‑2‑like 2 (Nrf2) / Kelch‑like ECH‑associated protein 1 (Keap1) signaling is an antioxidant pathway that plays an important role in protecting cells against oxidative stress. Here, we aimed to determine the possible association between the Keap1 variants and genetic susceptibility to preeclampsia.

Methods: In a case-control study, 150 preeclampsia patients and 150 women with normal pregnancy from Northern Iran were selected to evaluate the genotypes of Keap1 (rs11085735) using the polymerase chain reaction (PCR)-restriction length polymorphism (RFLP) method.

Results: A significant association between genotypes of Keap1 rs11085735 polymorphism with the renal function biomarkers and the risk of preeclampsia was not found. However, the aspartate aminotransferase (AST) level was higher in the presence of the Keap1 AA genotype compared to AC and CC genotypes. We found a significantly higher prevalence of gestational diabetes mellitus (GDM) in mild- and severe- preeclampsia and also hypothyroidism in severe preeclampsia compared to controls.

Conclusions: We found an association between preeclampsia with GDM and hypothyroidism. Our findings suggest that the Keap1rs11085735 polymorphism may not be a risk factor for susceptibility to preeclampsia in our studied population; however, this polymorphism could affect the activity of AST.
Full-Text [PDF 277 kb]   (124 Downloads)    
Type of Article: Original Article | Subject: Biochemistry
Received: 2022/10/28 | Accepted: 2022/11/15 | Published: 2022/12/31

References
1. Brown MA, Magee LA, Kenny LC, Karumanchi SA, McCarthy FP, Saito S, et al. The hypertensive disorders of pregnancy: ISSHP classification, diagnosis & management recommendations for international practice. Pregnancy Hypertens. 2018;13:291-310. https://doi.org/10.1016/j.preghy.2018.08.022 [DOI:10.1016/j.preghy.2018.05.004] [PMID]
2. Aouache R, Biquard L, Vaiman D, Miralles F. Oxidative stress in preeclampsia and placental diseases. Int J Mol Sci. 2018;19(5):1-29. [DOI:10.3390/ijms19051496] [PMID] [PMCID]
3. Weissgerber TL, Mudd LM. Preeclampsia and Diabetes. Curr Diab Rep. 2015;15(9):1-10. [DOI:10.1007/s11892-015-0579-4] [PMID] [PMCID]
4. Su X, Liu Y, Li G, Liu X, Huang S, Duan T, et al. Associations of Hypothyroxinemia With Risk of Preeclampsia-Eclampsia and Gestational Hypertension. Front Endocrinol (Lausanne). 2021;12( 777152):1-9. [DOI:10.3389/fendo.2021.777152] [PMID] [PMCID]
5. Singh CK, Chhabra G, Ndiaye MA, Garcia-Peterson LM, MacK NJ, Ahmad N. The Role of Sirtuins in Antioxidant and Redox Signaling. Antioxidants Redox Signal.2018;28(8):643-61. [DOI:10.1089/ars.2017.7290] [PMID] [PMCID]
6. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World allergy Organ J. 2012;5(1):9-19. [DOI:10.1097/WOX.0b013e3182439613] [PMID] [PMCID]
7. Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria J Med. 2018;54(4):287-93. [DOI:10.1016/j.ajme.2017.09.001]
8. Zhang DD. Mechanistic studies of the Nrf2-Keap1 signaling pathway. Drug Metab Rev. 2006;38(4):769-89. [DOI:10.1080/03602530600971974] [PMID]
9. Kaspar JW, Niture SK, Jaiswal AK. Nrf2:INrf2 (Keap1) signaling in oxidative stress. Free Radic Biol Med. 2009;47(9):1304-9. [DOI:10.1016/j.freeradbiomed.2009.07.035] [PMID] [PMCID]
10. Niture SK, Khatri R, Jaiswal AK. Regulation of Nrf2 - An update. Free Radic Biol Med. 2014;66:36-44. [DOI:10.1016/j.freeradbiomed.2013.02.008] [PMID] [PMCID]
11. Myatt L, Cui X. Oxidative stress in the placenta. Histochem Cell Biol. 2004;122(4):369-82. [DOI:10.1007/s00418-004-0677-x] [PMID]
12. Basu J, Bendek B, Agamasu E, Salafia CM, Mishra A, Benfield N, et al. Placental Oxidative Status throughout Normal Gestation in Women with Uncomplicated Pregnancies. Obstet Gynecol Int. 2015;2015(276095):1-6. [DOI:10.1155/2015/276095] [PMID] [PMCID]
13. Incalza MA, D'Oria R, Natalicchio A, Perrini S, Laviola L, Giorgino F. Oxidative stress and reactive oxygen species in endothelial dysfunction associated with cardiovascular and metabolic diseases. Vascul Pharmacol. 2018;100:1-19. [DOI:10.1016/j.vph.2017.05.005] [PMID]
14. Guller S. Role of the syncytium in placenta-mediated complications of preeclampsia. Thromb Res. 2009;124(4):389-92. [DOI:10.1016/j.thromres.2009.05.016] [PMID] [PMCID]
15. Lam C, Lim KH, Kang DH, Karumanchi SA. Uric acid and preeclampsia. Semin Nephrol. 2005;25(1):56-60. [DOI:10.1016/j.semnephrol.2004.09.009] [PMID]
16. Bainbridge SA, Roberts JM. Uric Acid as a Pathogenic Factor in Preeclampsia. Placenta. 2008;29(SUPPL A):S67-72. [DOI:10.1016/j.placenta.2007.11.001] [PMID] [PMCID]
17. Manjareeka M, Nanda S. Elevated levels of serum uric acid, creatinine or urea in preeclamptic women. Int J Med Sci Public Heal. 2013;2:43-7. [DOI:10.5455/ijmsph.2013.2.43-47]
18. Dacaj R, Izetbegovic S, Stojkanovic G, Dreshaj S. Elevated Liver Enzymes in Cases of Preeclampsia and Intrauterine Growth Restriction. Med Arch (Sarajevo, Bosnia Herzegovina). 2016;70:44-7. [DOI:10.5455/medarh.2016.70.44-47] [PMID] [PMCID]
19. Hartikainen JM, Tengström M, Winqvist R, Jukkola-Vuorinen A, Pylkäs K, Kosma VM, et al. KEAP1 genetic polymorphisms associate with breast cancer risk and survival outcomes. Clin Cancer Res. 2015;21(7):1591-601. [DOI:10.1158/1078-0432.CCR-14-1887] [PMID]
20. Testa A, Leonardis D, Spoto B, Sanguedolce MC, Parlongo RM, Pisano A, et al. A polymorphism in a major antiox idant gene (Kelch-like ECH-associated protein 1) predicts incident cardiovascular events in chronic kidney disease patients: An explorator y study. J Hypertens. 2016;34(5):928-34. [DOI:10.1097/HJH.0000000000000878] [PMID]

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