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Mohajer Tehran F, Mohtasham N, Ahmadi M, Shahabinejad M, Mohammadi* M. RT-qPCR Analysis of LAMP3 (CD208) Gene Expression in Oral Lichen Planus and Oral Squamous Cell Carcinoma. rbmb.net 2023; 12 (1) :36-41
URL: http://rbmb.net/article-1-1090-en.html
URL: http://rbmb.net/article-1-1090-en.html
Farnaz Mohajer Tehran 
, Nooshin Mohtasham , Mojtaba Ahmadi , Mehdi Shahabinejad * , Maryam Mohammadi*
, Nooshin Mohtasham , Mojtaba Ahmadi , Mehdi Shahabinejad * , Maryam Mohammadi*
Oral and Maxillofacial Pathology Department, Oral and Maxillofacial Diseases Research Center, Faculty of Dentistry, Mashhad University of Medical Sciences.
Abstract: (769 Views)
Background: Many new studies have been conducted on cellular proteins to use them as prognostic markers or in target therapy through determining the increase or decrease in their expression in the lichen planus and OSCC. LAMP3 protein is one of these proteins which has been recently considered. Thus, considering the unknown etiology of lichen planus, significance of their early diagnosis and treatment and lack of a suitable and final treatment for this disease and oral cancers, and preventing the progression of lichen planus, which can turn into OSCC, we decided to investigate the level of expression of this gene and its effect on the progression, study the connection between these two conditions and the probable factors contributing to their etiopathogenesis.
Methods: In this study, ninety-four paraffin blocks tissue samples of patients were obtained together with their demographic documents. LAMP3 expression was measured RT-qPCR method.
Results: The results show that there is not any significant difference between age and sex population of our study. in squamous cell carcinoma the amount of expression of LAMP3 was higher than lichen planus and healthy margin. Average LAMP3 Gene expression in grade III was higher than group grade I & II in which considering significant level of 5%, it is statistically significant.
Conclusions: According to the findings of this study, it can be concluded that the expression of the LAMP3 gene in SCC lesions is higher than in healthy tissue. Hence, LAMP3 gene expression can be used as a diagnostic biomarker.
Methods: In this study, ninety-four paraffin blocks tissue samples of patients were obtained together with their demographic documents. LAMP3 expression was measured RT-qPCR method.
Results: The results show that there is not any significant difference between age and sex population of our study. in squamous cell carcinoma the amount of expression of LAMP3 was higher than lichen planus and healthy margin. Average LAMP3 Gene expression in grade III was higher than group grade I & II in which considering significant level of 5%, it is statistically significant.
Conclusions: According to the findings of this study, it can be concluded that the expression of the LAMP3 gene in SCC lesions is higher than in healthy tissue. Hence, LAMP3 gene expression can be used as a diagnostic biomarker.
Type of Article: Original Article |
Subject:
Immunology
Received: 2022/11/6 | Accepted: 2023/03/31 | Published: 2023/08/15
Received: 2022/11/6 | Accepted: 2023/03/31 | Published: 2023/08/15
References
1. Neville BW, Damm DD, Allen CM, Chi AC. Oral and maxillofacial pathology. 4th ed. Elsevier Health Sciences: Missoouri, USA; 2015.
2. Ghazi N, Aali N, Shahrokhi VR, Mohajertehran F, Saghravanian N. Relative Expression of SOX2 and OCT4 in Oral Squamous Cell Carcinoma and Oral Epithelial Dysplasia. Rep Biochem Mol Biol. 2020;9(2):171-179. [DOI:10.29252/rbmb.9.2.171] [PMID] [PMCID]
3. Shahabinejad M, Zare R, Mohajertehran F, Amouzad Mahdiraji A. Cytokeratins (CK7 and CK20) Genes Expression Association with Clinicopathological Indices in Oral Squamous Cell Carcinoma and Dysplastic Oral Epithelium. Rep Biochem Mol Biol. 2021;10(1):126-134. [DOI:10.52547/rbmb.10.1.126] [PMID] [PMCID]
4. Mohtasham N, Shahabinejad M, Kafiroudi S, Mohajertehran F. Evaluation of the Altered Tissue Expression of HSP60 and HSP70 Genes in Oral and Cutaneous Lichen Planus Compared to Normal Healthy Tissues. Indian J Dermatol. 2021 Nov-Dec;66(6):591-597. [DOI:10.4103/ijd.ijd_1060_20] [PMID] [PMCID]
5. Ni YH, Huang XF, Ding L, Wang ZY, Hu QG, Hou YY. Accumulation of CD208⁺ mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients. J Oral Maxillofac Surg. 2014;72(11):2178-85. [DOI:10.1016/j.joms.2014.04.038] [PMID]
6. Lu J, Ma H, Lian S, Huang D, Lian M, Zhang Y, Huang J, Feng X. Clinical Significance and Prognostic Value of the Expression of LAMP3 in Oral Squamous Cell Carcinoma. Dis Markers. 2017;2017:1218254. [DOI:10.1155/2017/1218254] [PMID] [PMCID]
7. Nishimura J, Tanaka H, Yamakoshi Y, Hiramatsu S, Tamura T, Toyokawa T, et al. Impact of tumor-infiltrating LAMP-3 dendritic cells on the prognosis of esophageal squamous cell carcinoma. Esophagus. 2019;16(4):333-344. [DOI:10.1007/s10388-019-00669-w] [PMID]
8. Santoro A, Majorana A, Roversi L, Gentili F, Marrelli S, Vermi W, et al. Recruitment of dendritic cells in oral lichen planus. J Pathol. 2005;205(4):426-34. [DOI:10.1002/path.1699] [PMID]
9. Qiu X, You Y, Huang J, Wang X, Zhu H, Wang Z. LAMP3 and TP53 overexpression predicts poor outcome in laryngeal squamous cell carcinoma. Int J Clin Exp Pathol. 2015;8(5):5519-27.
10. Nagelkerke A, Sweep FC, Stegeman H, Grénman R, Kaanders JH, Bussink J, Span PN. Hypoxic regulation of the PERK/ATF4/LAMP3-arm of the unfolded protein response in head and neck squamous cell carcinoma. Head Neck. 2015 Jun;37(6):896-905. [DOI:10.1002/hed.23693] [PMID]
11. Terkelsen T, Russo F, Gromov P, Haakensen VD, Brunak S, Gromova I et al. Secreted breast tumor interstitial fluid microRNAs and their target genes are associated with triple-negative breast cancer, tumor grade, and immune infiltration. Breast Cancer Res. 2020;22(1):73. [DOI:10.1186/s13058-020-01295-6] [PMID] [PMCID]
12. Tanaka T, Warner BM, Odani T, Ji Y, Mo YQ, Nakamura H, et al. LAMP3 induces apoptosis and autoantigen release in Sjögren's syndrome patients. Sci Rep. 2020;10(1):15169. [DOI:10.1038/s41598-020-71669-5] [PMID] [PMCID]
13. Kanao H, Enomoto T, Kimura T, Fujita M, Nakashima R, Ueda Y, et al. Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer. Cancer Res. 2005;65(19):8640-5. [DOI:10.1158/0008-5472.CAN-04-4112] [PMID]
14. Liao X, Chen Y, Liu D, Li F, Li X, Jia W. High Expression of LAMP3 Is a Novel Biomarker of Poor Prognosis in Patients with Esophageal Squamous Cell Carcinoma. Int J Mol Sci. 2015;16(8):17655-67. [DOI:10.3390/ijms160817655] [PMID] [PMCID]
15. Alessandrini F, Pezzè L, Ciribilli Y. LAMPs: Shedding light on cancer biology. Semin Oncol. 2017;44(4):239-253. [DOI:10.1053/j.seminoncol.2017.10.013] [PMID]
16. Saitoh O, Wang WC, Lotan R, Fukuda M. Differential glycosylation and cell surface expression of lysosomal membrane glycoproteins in sublines of a human colon cancer exhibiting distinct metastatic potentials. J Biol Chem. 1992;267(8):5700-11. [DOI:10.1016/S0021-9258(18)42823-2] [PMID]
17. Lu J, Ma H, Lian S, Huang D, Lian M, Zhang Y, et al. Clinical Significance and Prognostic Value of the Expression of LAMP3 in Oral Squamous Cell Carcinoma. Dis Markers. 2017;2017:1218254. [DOI:10.1155/2017/1218254] [PMID] [PMCID]
18. Sun R, Wang X, Zhu H, Mei H, Wang W, Zhang S, Huang J. Prognostic value of LAMP3 and TP53 overexpression in benign and malignant gastrointestinal tissues. Oncotarget. 2014;5(23):12398-409. [DOI:10.18632/oncotarget.2643] [PMID] [PMCID]
19. Nagelkerke A, Mujcic H, Bussink J, Wouters BG, van Laarhoven HW, Sweep FC, Span PN. Hypoxic regulation and prognostic value of LAMP3 expression in breast cancer. Cancer. 2011;117(16):3670-81. [DOI:10.1002/cncr.25938] [PMID]
20. Nagelkerke A, Sieuwerts AM, Bussink J, Sweep FC, Look MP, Foekens JA, et al. LAMP3 is involved in tamoxifen resistance in breast cancer cells through the modulation of autophagy. Endocr Relat Cancer. 2014;21(1):101-12. [DOI:10.1530/ERC-13-0183] [PMID]
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