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Mirsanei J S, Nazari M, Shabani R, Govahi A, Eghbali S, Ajdary M, et al . Does Gold-Silver Core-Shell Nanostructure with Alginate Coating Induce Apoptosis in Human Lymphoblastic Tumoral (Jurkat) Cell Line?. rbmb.net 2023; 12 (2) :233-240
URL: http://rbmb.net/article-1-1127-en.html
URL: http://rbmb.net/article-1-1127-en.html
Jamileh Sadat Mirsanei 
, Mahsa Nazari , Ronak Shabani , Azam Govahi , Sahar Eghbali , Marziyeh Ajdary , Rana Mehdizadeh , Atieh Sadat Mousavi , Mehdi Mehdizadeh *
, Mahsa Nazari , Ronak Shabani , Azam Govahi , Sahar Eghbali , Marziyeh Ajdary , Rana Mehdizadeh , Atieh Sadat Mousavi , Mehdi Mehdizadeh *
Reproductive Sciences and Technology Research Center, Department of Anatomy, Iran University of Medical Sciences, Tehran, Iran.
Abstract: (1506 Views)
Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an 80% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U251 cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis.
Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for 24, 48, and 72 hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of 75 µg/ml for 48 hours according to the MTT results. After 48 hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-2, and CASPASE-3).
Results: According to our results, the rod-shaped silver nanostructures had a size of about 50 nm, increased apoptotic markers, including BAX and CASPASE-3, and induced cell death.
Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.
Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an 80% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U251 cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis.
Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for 24, 48, and 72 hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of 75 µg/ml for 48 hours according to the MTT results. After 48 hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-2, and CASPASE-3).
Results: According to our results, the rod-shaped silver nanostructures had a size of about 50 nm, increased apoptotic markers, including BAX and CASPASE-3, and induced cell death.
Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.
Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for 24, 48, and 72 hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of 75 µg/ml for 48 hours according to the MTT results. After 48 hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-2, and CASPASE-3).
Results: According to our results, the rod-shaped silver nanostructures had a size of about 50 nm, increased apoptotic markers, including BAX and CASPASE-3, and induced cell death.
Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.
Background: T-cell acute lymphoblastic leukemia (T-ALL) is known as an aggressive malignant disease resulting from the neoplastic alteration of T precursor cells. Although treatment with stringent chemotherapy regimens has achieved an 80% cure rate in children, it has been associated with lower success rates in adult treatment. Silver nanoparticles (Ag-NPs) have a toxic effect on human breast cancer cells, human glioblastoma U251 cells, and chronic myeloid leukemia cells in vitro. This study aimed to investigate the effect of Ag nanostructures (Ag-NSs) on Jurkat cells’ viability and apoptosis.
Methods: The Jurkat cell line was acquired. Following the synthesis Ag-NSs and their characterization, they were incubated with Jurkat cells at different doses for 24, 48, and 72 hours to determine the optimal time and dose. Two groups were examined: a control group with Jurkat cells without nanostructure maintained in the same medium as the cells in the treatment group without changing the medium, and a treatment group with cells treated with the Ag nanostructure solution at a dose of 75 µg/ml for 48 hours according to the MTT results. After 48 hours, the cells from the two groups were used for the q RT-PCR of the apoptotic genes (BAX, BCL-2, and CASPASE-3).
Results: According to our results, the rod-shaped silver nanostructures had a size of about 50 nm, increased apoptotic markers, including BAX and CASPASE-3, and induced cell death.
Conclusions: Ag-NSs have anticancer properties and can induce apoptosis of cells; therefore, they may be a potential candidate for the treatment of T-cell acute lymphoblastic leukemia.
Type of Article: Original Article |
Subject:
Cell Biology
Received: 2023/01/14 | Accepted: 2023/09/24 | Published: 2023/12/20
Received: 2023/01/14 | Accepted: 2023/09/24 | Published: 2023/12/20
References
1. Iacobucci I, Mullighan CG. Genetic Basis of Acute Lymphoblastic Leukemia. J Clin Oncol. 2017;35(9):975-983. [DOI:10.1200/JCO.2016.70.7836] [PMID] []
2. Johansson B, Harrison CJ. Acute myeloid leukemia. In: Heim S, Mitelman F, editors. Cancer Cytogenetics. 4th ed. Hoboken: Wiley-Blackwell; 2015;62-125 [DOI:10.1002/9781118795569.ch6]
3. Hefazi M, Litzow MR. Recent Advances in the Biology and Treatment of T Cell Acute Lymphoblastic Leukemia. Curr Hematol Malig Rep. 2018;13(4):265-274. [DOI:10.1007/s11899-018-0455-9] [PMID]
4. Bene MC, Castoldi G, Knapp W, Ludwig WD, Matutes E, Orfao A, van't Veer MB. Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL). Leukemia. 1995;9(10):1783-6.
5. Pui CH, Thiel E. Central nervous system disease in hematologic malignancies: historical perspective and practical applications. Semin Oncol. 2009;36(4 Suppl 2):S2-S16. [DOI:10.1053/j.seminoncol.2009.05.002] [PMID] []
6. Ghodousi-Dehnavi E, Arjmand M, Akbari Z, Aminzadeh M, Haji Hosseini R. Anti-Cancer Effect of Dorema Ammoniacum Gum by Targeting Metabolic Reprogramming by Regulating APC, P53, KRAS Gene Expression in HT-29 Human Colon Cancer Cells. Rep Biochem Mol Biol. 2023;12(1):127-135.
7. Rezai M, Saravani R, Sargazi S, Moudi M, Jafari Shahroudi M, Saravani R. Achillea Wilhelmsii C. KochHydroalcoholic Extract Induces Apoptosis and Alters LIN28B and p53 Gene Expression in Hela Cervical Cancer Cells. Rep Biochem Mol Biol. 2019;8(3):318-325.
8. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007;35(4):495-516. [DOI:10.1080/01926230701320337] [PMID] []
9. Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N. Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta. 2013;1833(12):3448-3459. [DOI:10.1016/j.bbamcr.2013.06.001] [PMID]
10. Gholipour H, Lahijani MS. Teratogenic Effects of Two New Derivatives of Quinazolinones on Balb/C Mice Embryos and Newborns: A Literature Review. Curr Res Biol. 2017;9(2):23-31. [DOI:10.19026/crjbs.9.5139]
11. Chipuk JE, Moldoveanu T, Llambi F, Parsons MJ, Green DR. The BCL-2 family re:union:. Mol Cell. 2010;37(3):299-310. [DOI:10.1016/j.molcel.2010.01.025] [PMID] []
12. Sánchez-López E, Gomes D, Esteruelas G, Bonilla L, Lopez-Machado AL, Galindo R, et al. Metal-Based Nanoparticles as Antimicrobial Agents: An Overview. Nanomaterials (Basel). 2020;10(2):292. [DOI:10.3390/nano10020292] [PMID] []
13. Sofi MA, Sunitha S, Sofi MA, Pasha SKK, Choi D. An overview of antimicrobial and anticancer potential of silver nanoparticles. J King Saud Univ Sci. 2022;34 (2).101791. [DOI:10.1016/j.jksus.2021.101791]
14. Cheng X, Zhang W, Ji Y, Meng J, Guo H, Liu J, et al. Revealing silver cytotoxicity using Au nanorods/Ag shell nanostructures: disrupting cell membrane and causing apoptosis through oxidative damage. RSC Adv. 2013;3(7):2296-305. [DOI:10.1039/c2ra23131j]
15. Mikhailova EO. Silver Nanoparticles: Mechanism of Action and Probable Bio-Application. J Funct Biomater. 2020;11(4):84. [DOI:10.3390/jfb11040084] [PMID] []
16. Franco-Molina MA, Mendoza-Gamboa E, Sierra-Rivera CA, Gómez-Flores RA, Zapata-Benavides P, Castillo-Tello P, et al. Antitumor activity of colloidal silver on MCF-7 human breast cancer cells. J Exper Clin Cancer Res. 2010;29(1):148. [DOI:10.1186/1756-9966-29-148] [PMID] []
17. Asharani P, Hande MP, Valiyaveettil S. Anti-proliferative activity of silver nanoparticles. BMC Cell Biol. 2009;10(65):1-14. [DOI:10.1186/1471-2121-10-65] [PMID] []
18. Guo D, Zhao Y, Zhang Y, Wang Q, Huang Z, Ding Q, et al. The cellular uptake and cytotoxic effect of silver nanoparticles on chronic myeloid leukemia cells. J Biomed Nanotechnol. 2014;10(4):669-78. [DOI:10.1166/jbn.2014.1625] [PMID]
19. Lee YH, Cheng FY, Chiu HW, Tsai JC, Fang CY, Chen CW, Wang YJ. Cytotoxicity, oxidative stress, apoptosis and the autophagic effects of silver nanoparticles in mouse embryonic fibroblasts. Biomaterials. 2014;35(16):4706-15. [DOI:10.1016/j.biomaterials.2014.02.021] [PMID]
20. Nazari M, Shabani R, Ajdary M, Ashjari M, Shirazi R, Govahi A, Kermanian F, Mehdizadeh M. Effects of Au@Ag core-shell nanostructure with alginate coating on male reproductive system in mice. Toxicol Rep. 2023;10:104-116. [DOI:10.1016/j.toxrep.2023.01.003] [PMID] []
21. Govahi A, Amjadi F, Nasr-Esfahani MH, Raoufi E, Mehdizadeh M. Accompaniment of Time-Lapse Parameters and Cumulus Cell RNA-Sequencing in Embryo Evaluation. Reprod Sci. 2022;29(2):395-409.
https://doi.org/10.1007/s43032-021-00811-z [DOI:10.1007/s43032-021-00748-3]
22. Chiaretti S, Gianfelici V, O'Brien SM, Mullighan CG. Advances in the Genetics and Therapy of Acute Lymphoblastic Leukemia. Am Soc Clin Oncol Educ Book. 2016;35:e314-22. [DOI:10.1200/EDBK_156628] [PMID]
23. Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. Int J Mol Sci. 2016;17(9):1534. [DOI:10.3390/ijms17091534] [PMID] []
24. Anoop NV, Jacob R, Paulson JM, Dineshkumar B, Narayana CR. Mango leaf extract synthesized silver nanorods exert anticancer activity on breast cancer and colorectal carcinoma cells. J Drug Deliv Sci Technol. 2018;44:8-12. [DOI:10.1016/j.jddst.2017.11.023]
25. Steckiewicz KP, Barcinska E, Malankowska A, Zauszkiewicz-Pawlak A, Nowaczyk G, Zaleska-Medynska A, Inkielewicz-Stepniak I. Impact of gold nanoparticles shape on their cytotoxicity against human osteoblast and osteosarcoma in in vitro model. Evaluation of the safety of use and anti-cancer potential. J Mater Sci Mater Med. 2019;30(2):22. [DOI:10.1007/s10856-019-6221-2] [PMID] []
26. Zhou G, Wang W. Synthesis of silver nanoparticles and their antiproliferation against human lung cancer cells in vitro. Orient J Chem 2012;28(2):651-55. [DOI:10.13005/ojc/280204]
27. Baharara J, Namvar F, Ramezani T, Mousavi M, Mohamad R. Silver nanoparticles biosynthesized using Achillea biebersteinii flower extract: apoptosis induction in MCF-7 cells via caspase activation and regulation of Bax and Bcl-2 gene expression. Molecules. 2015;20(2):2693-706. [DOI:10.3390/molecules20022693] [PMID] []
28. Mollick MMR, Rana D, Dash SK, Chattopadhyay S, Bhowmick B, Maity D, et al. Studies on green synthesized silver nanoparticles using Abelmoschus esculentus (L.) pulp extract having anticancer (in vitro) and antimicrobial applications. Arab J Chem. 2019;12(8):2572-84. [DOI:10.1016/j.arabjc.2015.04.033]
29. Ahmadian E, Dizaj SM, Rahimpour E, Hasanzadeh A, Eftekhari A, Hosain Zadegan H, et al. Effect of silver nanoparticles in the induction of apoptosis on human hepatocellular carcinoma (HepG2) cell line. Mater Sci Eng C Mater Biol Appl. 2018;93:465-471. [DOI:10.1016/j.msec.2018.08.027] [PMID]
30. Porter AG, Jänicke RU. Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 1999 Feb;6(2):99-104. [DOI:10.1038/sj.cdd.4400476] [PMID]
31. Singh R, Letai A, Sarosiek K. Regulation of apoptosis in health and disease: the balancing act of BCL-2 family proteins. Nat Rev Mol Cell Biol. 2019;20(3):175-193. [DOI:10.1038/s41580-018-0089-8] [PMID] []
32. Ghooshchian M, Khodarahmi P, Tafvizi F. Apoptosis-mediated neurotoxicity and altered gene expression induced by silver nanoparticles. Toxicology and Industrial Health. 2017;33(10):757-764. [DOI:10.1177/0748233717719195] [PMID]
33. Gross A. BCL-2 proteins: regulators of the mitochondrial apoptotic program. IUBMB Life. 2001;52(3-5):231-6. [DOI:10.1080/15216540152846046] [PMID]
34. Renault TT, Dejean LM, Manon S. A brewing understanding of the regulation of Bax function by Bcl-xL and Bcl-2. Mech Ageing Dev. 2017;161(Pt B):201-210. [DOI:10.1016/j.mad.2016.04.007] [PMID]
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