Volume 12, Issue 1 (Vol.12 No.1 Apr 2023)                   rbmb.net 2023, 12(1): 205-210 | Back to browse issues page

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Chismirina S, Sungkar S, Adlim M, Darmawi D. Streptococcus Mutans Serotype Analysis from Dental Plaque of Caries Patients in Banda Aceh Based on the GTF Gene. rbmb.net 2023; 12 (1) :205-210
URL: http://rbmb.net/article-1-1160-en.html
Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh 23111, Aceh, Indonesia.
Abstract:   (1144 Views)
Background: Dental caries is an oral disease that is widely suffered by the population of Aceh caused by Streptococcus mutans. S. mutans serotypes c and d are widely isolated in the human oral cavity. This research was focused on detecting the presence and variability of S. mutans in supragingival dental plaque of caries teenager and young adults’ patients.

Methods: Subjects involved in this study were patients who treated at the Rumah Sakit Gigi dan Mulut of Dentistry Faculty of Universitas Syiah Kuala. The approach used in this research was molecular microbiology technique. To determine the presence of S. mutans, supragingival plaque from caries patients was cultivated in TYS20B. The culture findings were utilized to detect the presence of bacteria using PCR. The primers utilized in the PCR were S. mutans specific primers, GTFB (517 bp) for S. mutans serotype c and GTFI (712 bp) for S. mutans serotype d.

Results: Culture results on TYS20B media showed the growth of S. mutans colonies isolated from the supragingival plaque of research subjects. PCR results also revealed the presence of S. mutans in the supragingival plaques of caries patients, with the variability of S. mutans discovered to be a serotype c and a serotype d.

Conclusions: Based on the findings of this study, it can be concluded that S. mutans can be found in the supragingival plaques of caries patients with the serotypes c and d variability.
Full-Text [PDF 247 kb]   (898 Downloads)    
Type of Article: Original Article | Subject: Microbiology
Received: 2023/04/11 | Accepted: 2023/06/27 | Published: 2023/08/15

1. Cianetti S, Lombardo G, Lupatelli E, Rossi G, Abraha I, Pagano S, Paglia L. Dental caries, parents educational level, family income and dental service attendance among children in Italy. Eur J Paediatr Dent. 2017;18(1):15-18.
2. Esberg A, Sheng N, Mårell L, Claesson R, Persson K, Borén T, Strömberg N. Streptococcus Mutans Adhesin Biotypes that Match and Predict Individual Caries Development. EBioMedicine. 2017;24:205-215. [DOI:10.1016/j.ebiom.2017.09.027] [PMID] [PMCID]
3. Xu H, Tian J, Hao W, Zhang Q, Zhou Q, Shi W, et al. Oral Microbiome Shifts From Caries-Free to Caries-Affected Status in 3-Year-Old Chinese Children: A Longitudinal Study. Front Microbiol. 2018;9:2009. [DOI:10.3389/fmicb.2018.02009] [PMID] [PMCID]
4. Nanda J, Sachdev V, Sandhu M, Deep-Singh-Nanda K. Correlation between dental caries experience and mutans streptococci counts using saliva and plaque as microbial risk indicators in 3- 8- year-old children. A cross
5. sectional study. J Clin Exp Dent. 2015;7(1):e114-8.
6. Nishana E, Bhat SS, Sahana KS, Hegde SK, Bhat V, Kalal BS. Estimation of Salivary sCD14 in Children with Early Childhood Caries in Association with Pneumonia. Rep Biochem Mol Biol. 2019;8(2):132-138.
7. Damle SG, Loomba A, Dhindsa A, Loomba A, Beniwal, V. Correlation between Dental Caries Experience and Mutans Streptococci Counts by Microbial and Molecular (Polymerase Chain Reaction) Assay using Saliva as Microbial Risk Indicator. Dent Res J. 2016:13(6):552-9. [DOI:10.4103/1735-3327.197035] [PMID] [PMCID]
8. Soyolmaa M, Munguntsetseg L, Sharkhuu MO, Nishino M, Hulan U. PCR detection of Streptococcus mutans and Streptococcus sobrinus in Plaque Samples from Mongolian Mother-Child Pairs. Pediatr Dent J. 2011;21(2):154-159. [DOI:10.1016/S0917-2394(11)70242-7]
9. Lemos JA, Palmer SR, Zeng L, Wen ZT, Kajfasz JK, et al. The Biology of Streptococcus mutans. Microbiol Spectr. 2019;7(1):1-26. [DOI:10.1128/microbiolspec.GPP3-0051-2018] [PMID] [PMCID]
10. Takahashi N, Nyvad B. The role of bacteria in the caries process: ecological perspectives. J Dent Res. 2011;90(3):294-303. [DOI:10.1177/0022034510379602] [PMID]
11. Cunha-Cruz J, Scott J, Rothen M, Mancl L, Lawhorn T, Brossel K, Berg J; Northwest Practice-based REsearch Collaborative in Evidence-based DENTistry. Salivary characteristics and dental caries: evidence from general dental practices. J Am Dent Assoc. 2013;144(5):e31-40. [DOI:10.14219/jada.archive.2013.0159] [PMID] [PMCID]
12. Martins C, Buczynski AK, Maia LC, Siqueira WL, Castro GF. Salivary proteins as a biomarker for dental caries--a systematic review. J Dent. 2013;41(1):2-8. [DOI:10.1016/j.jdent.2012.10.015] [PMID]
13. Freires IA, Avilés-Reyes A, Kitten T, Simpson-Haidaris PJ, Swartz M, Knight PA, et al. Heterologous expression of Streptococcus mutans Cnm in Lactococcus lactis promotes intracellular invasion, adhesion to human cardiac tissues and virulence. Virulence. 2017;8(1):18-29. [DOI:10.1080/21505594.2016.1195538] [PMID] [PMCID]
14. Zero DT, Fontana M, Martínez-Mier EA, Ferreira-Zandoná A, Ando M, González-Cabezas C, Bayne S. The biology, prevention, diagnosis and treatment of dental caries: scientific advances in the United States. J Am Dent Assoc. 2009;140 Suppl 1:25S-34S. [DOI:10.14219/jada.archive.2009.0355] [PMID]
15. Khan A, Qureshi B, Qureshi A, Imtiaz Y, Qadeer S. Correlation of Salivary Characteristics with High Risk of Dental Caries; A Clinical Investigation. Future Dent J. 2018;4(1):72-75. [DOI:10.1016/j.fdj.2017.10.002]
16. Stookey GK. The Effect of Saliva on Dental Caries. J Am Dent Assoc. 2008;139: 139 Suppl:11S-17S. [DOI:10.14219/jada.archive.2008.0347] [PMID]
17. Karaoğlanoğlu S, Akgül N, Akgül HM. The Association Between the DMFS Index and Levels of Salivary Streptococcus Mutans and Lactobacilli of Subjects Living in Erzurum, Turkey. J Dent Sci. 2010;5(2):70-74. [DOI:10.1016/S1991-7902(10)60011-6]
18. Smith DJ. Dental caries vaccines: prospects and concerns. Crit Rev Oral Biol Med. 2002;13(4):335-349. [DOI:10.1177/154411130201300404] [PMID]
19. Hancock RE, Sahl HG. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat Biotechnol. 2006;24(12):1551-7. [DOI:10.1038/nbt1267] [PMID]
20. Aas JA, Griffen AL, Dardis SR, Lee AM, Olsen I, Dewhirst FE, et al. Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults. J Clin Microbiol. 2008;46(4):1407-1417. [DOI:10.1128/JCM.01410-07] [PMID] [PMCID]
21. Niessen LC, Gibson G, Kinnunen TH. Women's oral health: why sex and gender matter. Dent Clin North Am. 2013;57(2):181-94. [DOI:10.1016/j.cden.2013.02.004] [PMID]
22. Hamada S, Slade HD. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol Rev. 1980;44(2):331-84. [DOI:10.1128/mr.44.2.331-384.1980] [PMID] [PMCID]
23. Shukla C, Maurya RK, Singh V, Tijare M. Evaluation of changes in Streptococcus mutans colonies in microflora of the Indian population with fixed orthodontics appliances. Dent Res J (Isfahan). 2016;13(4):309-14. [DOI:10.4103/1735-3327.187876] [PMID] [PMCID]
24. Jeevarathan J, Deepti A, Muthu MS, Rathna Prabhu V, Chamundeeswari GS. Effect of fluoride varnish on Streptococcus mutans counts in plaque of caries-free children using Dentocult SM strip mutans test: a randomized controlled triple blind study. J Indian Soc Pedod Prev Dent. 2007;25(4):157-63. [DOI:10.4103/0970-4388.37010] [PMID]
25. Saravia ME, Nelson-Filho P, Ito IY, da Silva LA, da Silva RA, Emilson CG. Morphological differentiation between S. mutans and S. sobrinus on modified SB-20 culture medium. Microbiol Res. 2011;166(1):63-7. [DOI:10.1016/j.micres.2010.01.001] [PMID]
26. Sánchez-Acedo M, Montiel-Company JM, Dasí-Fernández F, Almerich-Silla JM. Streptococcus mutans and Streptococcus sobrinus detection by Polymerase Chain Reaction and their relation to dental caries in 12 and 15 year-old schoolchildren in Valencia (Spain). Med Oral Patol Oral Cir Bucal. 2013;18(6):e839-45. [DOI:10.4317/medoral.18941] [PMID] [PMCID]
27. Banas JA, Drake DR. Are the mutans streptococci still considered relevant to understanding the microbial etiology of dental caries? BMC Oral Health. 2018;18(1):129. [DOI:10.1186/s12903-018-0595-2] [PMID] [PMCID]
28. Bowen WH, Koo H. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res. 2011;45(1):69-86. [DOI:10.1159/000324598] [PMID] [PMCID]

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