Reports of Biochemistry
and Molecular Biology
Fri, May 17, 2024
[Archive]
Volume 12, Issue 2 (Vol.12 No.2 Jul 2023)
rbmb.net 2023, 12(2): 241-250 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Namazi G, Heidar Beygi S, Vahidi M H, Asa P, Bahmani F, Mafi A et al . Relationship Between Red Cell Distribution Width and Oxidative Stress Indexes in Patients with Coronary Artery Disease. rbmb.net 2023; 12 (2) :241-250
URL: http://rbmb.net/article-1-1166-en.html
URL: http://rbmb.net/article-1-1166-en.html
Gholamreza Namazi * 
, Somayeh Heidar Beygi , Mohammad Hasan Vahidi , Parastoo Asa , Fereshteh Bahmani , Alireza Mafi , Fariba Rayegan
, Somayeh Heidar Beygi , Mohammad Hasan Vahidi , Parastoo Asa , Fereshteh Bahmani , Alireza Mafi , Fariba Rayegan
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran & Department of Clinical Biochemistry, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Abstract: (894 Views)
Background: Red blood cell distribution (RDW), an index of the size variability of erythrocytes, is significantly associated with coronary stenosis and can strongly predict the mortality risk in coronary artery disease (CAD). The biological mechanisms involved are not fully understood but may include oxidative stress. We sought to investigate the relationship between RDW and markers of oxidative stress in patients with CAD.
Methods: Participants were 112 consecutive patients referred to department of cardiac surgery for evaluation of chest pain. 32 patients had stable CAD, 40 patients had unstable CAD and 40 subjects were diagnosed as non-CAD. The levels of lipid peroxidation (TBARS) were measured in plasma and membrane samples by a fluorometric method. The plasma levels of glutathione (GSH) and total antioxidant capacity (TAC) were determined using spectrophotometric methods.
Results: Lipid peroxidation levels were significantly higher in the erythrocyte membrane of stable CAD patients than non-CAD patients. The levels of TAC were significantly lower in both stable and unstable groups when compared to that of the control group (P< 0.019 and P< 0.001, respectively), but did not differ between stable and unstable CAD. In addition, there was no significant difference in the serum GSH levels among the study groups. Membrane TBARS was directly associated with RDW in three groups of study.
Conclusions: We found an independent association between RDW levels and membrane lipid peroxidation in patients with CAD. This finding suggests that oxidative stress may be a potential underlying biological mechanism for increased RDW in CAD patients.
Methods: Participants were 112 consecutive patients referred to department of cardiac surgery for evaluation of chest pain. 32 patients had stable CAD, 40 patients had unstable CAD and 40 subjects were diagnosed as non-CAD. The levels of lipid peroxidation (TBARS) were measured in plasma and membrane samples by a fluorometric method. The plasma levels of glutathione (GSH) and total antioxidant capacity (TAC) were determined using spectrophotometric methods.
Results: Lipid peroxidation levels were significantly higher in the erythrocyte membrane of stable CAD patients than non-CAD patients. The levels of TAC were significantly lower in both stable and unstable groups when compared to that of the control group (P< 0.019 and P< 0.001, respectively), but did not differ between stable and unstable CAD. In addition, there was no significant difference in the serum GSH levels among the study groups. Membrane TBARS was directly associated with RDW in three groups of study.
Conclusions: We found an independent association between RDW levels and membrane lipid peroxidation in patients with CAD. This finding suggests that oxidative stress may be a potential underlying biological mechanism for increased RDW in CAD patients.
Keywords: Coronary stenosis, Oxidative stress, Red blood cell distribution (RDW), Total antioxidant capacity.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2023/04/24 | Accepted: 2023/09/4 | Published: 2023/12/20
Received: 2023/04/24 | Accepted: 2023/09/4 | Published: 2023/12/20
References
1. Korantzopoulos P, Roever L, Liu T. Red blood cell distribution width and atrial fibrillation. Biomark Med. 2020;14(13):1289-98. [DOI:10.2217/bmm-2020-0041] [PMID]
2. Salisbury AC, Amin AP, Reid KJ, Wang TY, Alexander KP, Chan PS, et al. Red blood cell indices and development of hospital-acquired anemia during acute myocardial infarction. American J Cardiol. 2012;109(8):1104-10. [DOI:10.1016/j.amjcard.2011.11.045] [PMID]
3. Arkew M, Gemechu K, Haile K, Asmerom H. Red Blood Cell Distribution Width as Novel Biomarker in Cardiovascular Diseases: A Literature Review. J Blood Med. 2022;13:413-24. [DOI:10.2147/JBM.S367660] [PMID] []
4. Nagula P, Karumuri S, Otikunta AN, Yerrabandi SRV. Correlation of red blood cell distribution width with the severity of coronary artery disease- A single center study. Indian Heart J. 2017;69(6):757-61. [DOI:10.1016/j.ihj.2017.04.007] [PMID] []
5. Akilli H, Kayrak M, Aribas A, Alibasic H, Yildirim O, Lutfi Sertdemir A, et al. The relationship between red blood cell distribution width and myocardial ischemia in dobutamine stress echocardiography. Coron Artery Dis. 2014;25(2):152-8. [DOI:10.1097/MCA.0000000000000068] [PMID]
6. Valenti AC, Vitolo M, Imberti JF, Malavasi VL, Boriani G. Red Cell Distribution Width: A Routinely Available Biomarker with Important Clinical Implications in Patients with Atrial Fibrillation. Curr Pharm Des. 2021;27(37):3901-12. [DOI:10.2174/1381612827666210211125847] [PMID]
7. Cheraghi M, Ahmadvand H, Maleki A, Babaeenezhad E, Shakiba S, Hassanzadeh F. Oxidative Stress Status and Liver Markers in Coronary Heart Disease. Rep Biochem Mol Biol. 2019;8(1):49-55.
8. Mohammadi A, Balizadeh Karami AR, Dehghan Mashtani V, Sahraei T, Bandani Tarashoki Z, Khattavian E, et al. Evaluation of Oxidative Stress, Apoptosis, and Expression of MicroRNA-208a and MicroRNA-1 in Cardiovascular Patients. Rep Biochem Mol Biol. 2021;10(2):183-96. [DOI:10.52547/rbmb.10.2.183] [PMID] []
9. Friedman JS, Lopez MF, Fleming MD, Rivera A, Martin FM, Welsh ML, et al. SOD2-deficiency anemia: protein oxidation and altered protein expression reveal targets of damage, stress response, and antioxidant responsiveness. Blood. 2004;104(8):2565-73. [DOI:10.1182/blood-2003-11-3858] [PMID]
10. Siavash M, Sadeghi M, Salarifar F, Amini M, Shojaee-Moradie F. Comparison of body mass index and waist/height ratio in predicting definite coronary artery disease. Ann Nutr Metab. 2008;53(3-4):162-6. [DOI:10.1159/000172977] [PMID]
11. Chauhan VP, Tsiouris JA, Chauhan A, Sheikh AM, Brown WT, Vaughan M. Increased oxidative stress and decreased activities of Ca(2+)/Mg(2+)-ATPase and Na(+)/K(+)-ATPase in the red blood cells of the hibernating black bear. Life Sci. 2002;71(2):153-61. [DOI:10.1016/S0024-3205(02)01619-3] [PMID]
12. DeLuise M, Flier JS. Functionally abnormal Na+-K+ pump in erythrocytes of a morbidly obese patient. J Clin Investig. 1982;69(1):38-44. [DOI:10.1172/JCI110439] [PMID] []
13. Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978;87(1):206-10. [DOI:10.1016/0003-2697(78)90586-9] [PMID]
14. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8. [DOI:10.1016/0003-2697(79)90738-3] [PMID]
15. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996;239(1):70-6. [DOI:10.1006/abio.1996.0292] [PMID]
16. Beutler E, Gelbart T. Plasma glutathione in health and in patients with malignant disease. J Lab Clin Med. 1985;105(5):581-4.
17. Patel KV, Ferrucci L, Ershler WB, Longo DL, Guralnik JM. Red blood cell distribution width and the risk of death in middle-aged and older adults. Arch Intern Med. 2009;169(5):515-23. [DOI:10.1001/archinternmed.2009.11] [PMID] []
18. Perlstein TS, Weuve J, Pfeffer MA, Beckman JA. Red blood cell distribution width and mortality risk in a community-based prospective cohort. Arch Intern Med. 2009;169(6):588-94. [DOI:10.1001/archinternmed.2009.55] [PMID] []
19. Weiss G, Goodnough LT. Anemia of chronic disease. N Engl J Med. 2005;352(10):1011-23. [DOI:10.1056/NEJMra041809] [PMID]
20. Semba RD, Patel KV, Ferrucci L, Sun K, Roy CN, Guralnik JM, et al. Serum antioxidants and inflammation predict red cell distribution width in older women: the Women's Health and Aging Study I. Clin Nutr (Edinburgh, Scotland). 2010;29(5):600-4. [DOI:10.1016/j.clnu.2010.03.001] [PMID] []
21. Abdel-Moneim A, Mahmoud B, Nabil A, Negeem Z. Correlation between oxidative stress and hematological profile abnormalities in diabetic nephropathy. Diabetes Metab Syndr. 2019;13(4):2365-73. [DOI:10.1016/j.dsx.2019.06.014] [PMID]
22. Zhao Z, Liu T, Li J, Yang W, Liu E, Li G. Elevated red cell distribution width level is associated with oxidative stress and inflammation in a canine model of rapid atrial pacing. Int J Cardiol. 2014;174(1):174-6. [DOI:10.1016/j.ijcard.2014.03.189] [PMID]
23. Natali A, L'Abbate A, Ferrannini E. Erythrocyte sedimentation rate, coronary atherosclerosis, and cardiac mortality. Eur Heart J. 2003;24(7):639-48. [DOI:10.1016/S0195-668X(02)00741-8] [PMID]
24. Andresdottir MB, Sigfusson N, Sigvaldason H, Gudnason V. Erythrocyte sedimentation rate, an independent predictor of coronary heart disease in men and women: The Reykjavik Study. Am J Epidemiol. 2003;158(9):844-51. [DOI:10.1093/aje/kwg222] [PMID]
25. Gillum RF, Mussolino ME, Makuc DM. Erythrocyte sedimentation rate and coronary heart disease: the NHANES I Epidemiologic Follow-up Study. J Clin Epidemiol. 1995;48(3):353-61. [DOI:10.1016/0895-4356(94)00156-K] [PMID]
26. Yayan J. Erythrocyte sedimentation rate as a marker for coronary heart disease. Vasc Health Risk Manag. 2012;8:219-23. [DOI:10.2147/VHRM.S29284] [PMID] []
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
