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Volume 12, Issue 3 (Vol.12 No.3 Oct 2023)
rbmb.net 2023, 12(3): 465-475 |
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Mohammadzadeh A, Naghizadeh H, Mosaddegh A, Astani A, Pouresmaeil O, Mardaneh J. Identification and Evaluation of Pathogenic Genes (traT, hly, aer, pap, and fimH) and Antibiotic Resistance Genes (blaTEM, blaSHV, and blaCTX) in Escherichia coli in Patients Referred to Gonabad Hospitals, Iran. rbmb.net 2023; 12 (3) :465-475
URL: http://rbmb.net/article-1-1230-en.html
URL: http://rbmb.net/article-1-1230-en.html
Alireza Mohammadzadeh 
, Hamid Naghizadeh , Ahmad Mosaddegh , Akram Astani , Omid Pouresmaeil , Jalal Mardaneh *
, Hamid Naghizadeh , Ahmad Mosaddegh , Akram Astani , Omid Pouresmaeil , Jalal Mardaneh *
Department of Microbiology, School of Medicine, Infectious Diseases Research Center, Gonabad University of Medical Sciences, Gonabad, Iran.
Abstract: (406 Views)
Background: Urinary tract infection (UTI) is one of the common bacterial infections. Escherichia coli is the most common cause of UTI. In this research, the prevalence of several virulence factors and beta-lactam resistance genes was investigated.
Methods: One hundred E. coli isolates were collected from patients’ specimens with UTI referred to Allame-Bohlol Gonabadi hospital. Polymerase chain reaction (PCR) was performed to identify five pathogenic genes (fimH, aer, pap, hly, traT) and three antibiotic resistance genes (blaTEM, blaCTX, blaSHV).
Results: The frequencies of blaSHV, blaTEM and blaCTX beta-lactamase genes among extended-spectrum-beta-lactamases (ESBLs) positive isolates were 11.1%, 48.1%, and 93.3%, respectively. A significant number of isolates were resistant to the most commonly used antibiotics.
Conclusion: Pathogenic genes may also increase the severity, progression, and expansion of urinary tract infections. Therefore, identifying these genes as critical controllers of illness can use for better manage the treatment.
Methods: One hundred E. coli isolates were collected from patients’ specimens with UTI referred to Allame-Bohlol Gonabadi hospital. Polymerase chain reaction (PCR) was performed to identify five pathogenic genes (fimH, aer, pap, hly, traT) and three antibiotic resistance genes (blaTEM, blaCTX, blaSHV).
Results: The frequencies of blaSHV, blaTEM and blaCTX beta-lactamase genes among extended-spectrum-beta-lactamases (ESBLs) positive isolates were 11.1%, 48.1%, and 93.3%, respectively. A significant number of isolates were resistant to the most commonly used antibiotics.
Conclusion: Pathogenic genes may also increase the severity, progression, and expansion of urinary tract infections. Therefore, identifying these genes as critical controllers of illness can use for better manage the treatment.
Type of Article: Original Article |
Subject:
Microbiology
Received: 2023/08/22 | Accepted: 2023/09/24 | Published: 2024/02/25
Received: 2023/08/22 | Accepted: 2023/09/24 | Published: 2024/02/25
References
1. Matinfar S, Ahmadi M, Sisakht AM, Sadeghi J, Javedansirat S. Phylogenetic and antibiotics resistance in extended-spectrum B-lactamase (ESBL) Uropathogenic Escherichia coli: An updated review. Gene Rep. 2021:101168. [DOI:10.1016/j.genrep.2021.101168]
2. Vihta KD, Stoesser N, Llewelyn MJ, Quan TP, Davies T, Fawcett NJ, et al. Trends over time in Escherichia coli bloodstream infections, urinary tract infections, and antibiotic susceptibilities in Oxfordshire, UK, 1998-2016: a study of electronic health records. Lancet Infect Dis. 2018;18(10):1138-49. [DOI:10.1016/S1473-3099(18)30353-0] [PMID]
3. Abernethy J, Guy R, Sheridan E, Hopkins S, Kiernan M, Wilcox M, et al. Epidemiology of Escherichia coli bacteraemia in England: results of an enhanced sentinel surveillance programme. J Hosp Infect. 2017;95(4):365-75. [DOI:10.1016/j.jhin.2016.12.008] [PMID]
4. van Hout D, Verschuuren TD, Bruijning-Verhagen PC, Bosch T, Schürch AC, Willems RJ, et al. Extended-spectrum beta-lactamase (ESBL)-producing and non-ESBL-producing Escherichia coli isolates causing bacteremia in the Netherlands (2014-2016) differ in clonal distribution, antimicrobial resistance gene and virulence gene content. PloS one. 2020;15(1):e0227604. [DOI:10.1371/journal.pone.0227604] [PMID] []
5. Malekzadegan Y, Rastegar E, Moradi M, Heidari H, Ebrahim-Saraie HS. Prevalence of quinolone-resistant uropathogenic Escherichia coli in a tertiary care hospital in south Iran. Infect Drug Resist. 2019;12:1683.
https://doi.org/10.2147/IDR.S206966 [DOI:10.2147/IDR.S222698] [PMID] []
6. Johnson JR, Russo TA. Extraintestinal pathogenic Escherichia coli:"the other bad E coli". J Lab Clin Med. 2002;139(3):155-62. [DOI:10.1067/mlc.2002.121550] [PMID]
7. Klein RD, Hultgren SJ. Urinary tract infections: microbial pathogenesis, host-pathogen interactions and new treatment strategies. Nat Rev Microbiol. 2020;18(4):211-26. [DOI:10.1038/s41579-020-0324-0] [PMID] []
8. Bliven KA, Maurelli AT. Evolution of Bacterial Pathogens Within the Human Host. Microbiol Spectr. 2016;4(1):10. [DOI:10.1128/microbiolspec.VMBF-0017-2015] [PMID] []
9. Calhau V, Domingues S, Ribeiro G, Mendonça N, Da Silva GJ. Interplay between pathogenicity island carriage, resistance profile and plasmid acquisition in uropathogenic Escherichia coli. J Med Microbiol. 2015;64(8):828-35. [DOI:10.1099/jmm.0.000104] [PMID]
10. Schreiber IV HL, Spaulding CN, Dodson KW, Livny J, Hultgren SJ. One size doesn't fit all: unraveling the diversity of factors and interactions that drive E. coli urovirulence. Ann Transl Med. 2017;5(2):28. [DOI:10.21037/atm.2016.12.73] [PMID] []
11. Jadhav S, Hussain A, Devi S, Kumar A, Parveen S, Gandham N, et al. Virulence characteristics and genetic affinities of multiple drug resistant uropathogenic Escherichia coli from a semi urban locality in India. PloS one. 2011;6(3):e18063. [DOI:10.1371/journal.pone.0018063] [PMID] []
12. Pobiega M, Wojkowska-Mach J, Chmielarczyk A, Romaniszyn D, Adamski P, Heczko PB, et al. Molecular characterization and drug resistance of Escherichia coli strains isolated from urine from long-term care facility residents in Cracow, Poland. Med Sci Monit. 2013;19:317-26. . [DOI:10.12659/MSM.883898] [PMID] []
13. Servin AL. Pathogenesis of Afa/Dr diffusely adhering Escherichia coli. Clin Microbiol Rev. 2005;18(2):264-92. [DOI:10.1128/CMR.18.2.264-292.2005] [PMID] []
14. Gally DL, Leathart J, Blomfield IC. Interaction of FimB and FimE with the fim switch that controls the phase variation of type 1 fimbriae in Escherichia coli K-12. Mol Microbiol. 1996;21(4):725-38. [DOI:10.1046/j.1365-2958.1996.311388.x] [PMID]
15. Firoozeh F, Saffari M, Neamati F, Zibaei M. Detection of virulence genes in Escherichia coli isolated from patients with cystitis and pyelonephritis. Int J Infect Dis. 2014;29:219-22. [DOI:10.1016/j.ijid.2014.03.1393] [PMID]
16. Köhler CD, Dobrindt U. What defines extraintestinal pathogenic Escherichia coli? Int J Med Microbiol. 2011;301(8):642-7. [DOI:10.1016/j.ijmm.2011.09.006] [PMID]
17. Ingle DJ, Tauschek M, Edwards DJ, Hocking DM, Pickard DJ, Azzopardi KI, et al. Evolution of atypical enteropathogenic E. coli by repeated acquisition of LEE pathogenicity island variants. Nat Microbiol. 2016;1:15010. [DOI:10.1038/nmicrobiol.2015.10] [PMID]
18. von Mentzer A, Connor TR, Wieler LH, Semmler T, Iguchi A, Thomson NR, et al. I Identification of enterotoxigenic Escherichia coli (ETEC) clades with long-term global distribution. Nat Genet. 2014;46(12):1321-6. [DOI:10.1038/ng.3145] [PMID]
19. Riley LW. Pandemic lineages of extraintestinal pathogenic Escherichia coli. Clin Microbiol Infect. 2014;20(5):380-90. [DOI:10.1111/1469-0691.12646] [PMID]
20. Kallonen T, Brodrick HJ, Harris SR, Corander J, Brown NM, Martin V, et al. Systematic longitudinal survey of invasive Escherichia coli in England demonstrates a stable population structure only transiently disturbed by the emergence of ST131. Genome Res. 2017;27(8):1437-49. [DOI:10.1101/gr.216606.116] [PMID] []
21. Fibke CD, Croxen MA, Geum HM, Glass M, Wong E, Avery BP, et al. Genomic Epidemiology of Major Extraintestinal Pathogenic Escherichia coli Lineages Causing Urinary Tract Infections in Young Women Across Canada. Open Forum Infect Dis. 2019;6(11):ofz431. [DOI:10.1093/ofid/ofz431] [PMID] []
22. Ben Zakour NL, Alsheikh-Hussain AS, Ashcroft MM, Khanh Nhu NT, Roberts LW, Stanton-Cook M, et al. Sequential Acquisition of Virulence and Fluoroquinolone Resistance Has Shaped the Evolution of Escherichia coli ST131. mBio. 2016;7(2):e00347-16. [DOI:10.1128/mBio.00347-16] [PMID] []
23. Woerther PL, Burdet C, Chachaty E, Andremont A. Trends in human fecal carriage of extended-spectrum β-lactamases in the community: toward the globalization of CTX-M. Clin Microbiol Rev. 2013;26(4):744-58. [DOI:10.1128/CMR.00023-13] [PMID] []
24. Suzuki S, Shibata N, Yamane K, Wachino J, Ito K, Arakawa Y. Change in the prevalence of extended-spectrum-beta-lactamase-producing Escherichia coli in Japan by clonal spread. J Antimicrob Chemother. 2009;63(1):72-9. [DOI:10.1093/jac/dkn463] [PMID]
25. Dayan N, Dabbah H, Weissman I, Aga I, Even L, Glikman D. Urinary tract infections caused by community-acquired extended-spectrum β-lactamase-producing and nonproducing bacteria: a comparative study. J Pediatr. 2013;163(5):1417-21. [DOI:10.1016/j.jpeds.2013.06.078] [PMID]
26. Pouresmaeil O, Zandi H, Kalantar-Neyestanaki D, Safaei S, Fatahi-Bafghi M, Vakili M. Molecular Identification of MefE and AmpC Resistance Genes in ATCC Bacteria. Avicenna J Clin Microbiol Infect. 2019;6(4): 142-143. [DOI:10.34172/ajcmi.2019.26]
27. Afsharnia M, Naraghi B, Mardaneh J, Kianmehr M, Biglari H. The data of Escherichia coli strains genes in different types of wastewater. Data Brief. 2018;21:763-766. [DOI:10.1016/j.dib.2018.08.167] [PMID] []
28. Martins LR, Pina SM, Simões RL, de Matos AJ, Rodrigues P, da Costa PM. Common phenotypic and genotypic antimicrobial resistance patterns found in a case study of multiresistant E. coli from cohabitant pets, humans, and household surfaces. J Environ Health. 2013;75(6):74-81.
29. Ranjbar R, Farahani O. The Prevalence of Virulence Genes and Virulotypes of Escherichia coli Strains Isolated from Hospital Wastewaters in Tehran, Iran. Iran J Public Health. 2018;47(5):713-719.
30. Kot B, Wicha J, Gruzewska A, Piechota M, Wolska K, Obrebska M. Virulence factors, biofilm-forming ability, and antimicrobial resistance of urinary Escherichia coli strains isolated from hospitalized patients. Turk J Med Sci. 2016;46(6):1908-14. [DOI:10.3906/sag-1508-105] [PMID]
31. Tarchouna M, Ferjani A, Ben-Selma W, Boukadida J. Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection. Int J Infect Dis. 2013;17(6):e450-3. [DOI:10.1016/j.ijid.2013.01.025] [PMID]
32. Djuikoue IC, Woerther PL, Toukam M, Burdet C, Ruppé E, Gonsu KH, et al. Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon. J Infect Dev Ctries. 2016;10(10):1135-1139. [DOI:10.3855/jidc.7616] [PMID]
33. Keikha M, Rava M. Trend of antibiotic resistance of Escherichia coli strains isolated from urinary tract infections in outpatient patients from Zahedan. J Paramed Sci Rehab. 2017;6(4):73-8.
34. Hussain M, Hasan F, Shah AA, Hameed A, Jung M, Rayamajhi N, et al. Prevalence of class A and AmpC β-lactamases in clinical Escherichia coli isolates from Pakistan Institute of Medical Science, Islamabad, Pakistan. Jpn J Infect Dis. 2011;64(3):249-52. [DOI:10.7883/yoken.64.249] [PMID]
35. Haghighatpanah M, Mojtahedi A. Characterization of antibiotic resistance and virulence factors of Escherichia coli strains isolated from Iranian inpatients with urinary tract infections. Infect Drug Resist. 2019;12:2747-2754. [DOI:10.2147/IDR.S219696] [PMID] []
36. Bagheri Joshagahani S, Moniri R, Baghbani Taheri F, Sadat S, Heidarzadeh Z. Prevalence of serum antibodies to TORCH infection in the first trimester of the pregnancy in Kashan, Iran. Iran J Neonatol. 2015;6(1):8-12.
37. Oliveira FA, Paludo KS, Arend LN, Farah SM, Pedrosa FO, Souza EM, et al. Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains. Genet Mol Res. 2011;10(4):4114-25. [DOI:10.4238/2011.October.31.5] [PMID]
38. Garofalo CK, Hooton TM, Martin SM, Stamm WE, Palermo JJ, Gordon JI, Hultgren SJ. Escherichia coli from urine of female patients with urinary tract infections is competent for intracellular bacterial community formation. Infect Immun. 2007;75(1):52-60. [DOI:10.1128/IAI.01123-06] [PMID] []
39. Watts RE, Hancock V, Ong CL, Vejborg RM, Mabbett AN, Totsika M, et al. Escherichia coli isolates causing asymptomatic bacteriuria in catheterized and noncatheterized individuals possess similar virulence properties. J Clin Microbiol. 2010;48(7):2449-58. [DOI:10.1128/JCM.01611-09] [PMID] []
40. Usein CR, Damian M, Tatu-Chitoiu D, Capusa C, Fagaras R, Tudorache D, et al. Prevalence of virulence genes in Escherichia coli strains isolated from Romanian adult urinary tract infection cases. J Cell Mol Med. 2001;5(3):303-10. [DOI:10.1111/j.1582-4934.2001.tb00164.x] [PMID] []
41. Mladin C, Usein CR, Chifiriuc MC, Palade A, Slavu CL, Negut M, et al. Genetic analysis of virulence and pathogenicity features of uropathogenic Escherichia coli isolated from patients with neurogenic bladder. Rom Biotechnol Lett. 2009;14(6):4906-11.
42. Lago A, Fuentefria SR, Fuentefria DB. Enterobactérias produtoras de ESBL em Passo Fundo, estado do Rio Grande do Sul, Brasil [ESBL-producing enterobacteria in Passo Fundo, state of Rio Grande do Sul, Brazil]. Rev Soc Bras Med Trop. 2010;43(4):430-4. [DOI:10.1590/S0037-86822010000400019] [PMID]
43. Ghavidel M, Gholamhosseini-Moghadam T, Nourian K, Ghazvini K. Virulence factors analysis and antibiotic resistance of uropathogenic Escherichia coli isolated from patients in northeast of Iran. Iran J Microbiol. 2020;12(3):223-230. [DOI:10.18502/ijm.v12i3.3240] [PMID] []
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