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Volume 12, Issue 4 (Vol.12 No.4 Jan 2024)
rbmb.net 2024, 12(4): 631-642 |
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Mirderikvand A, Shahsavari G, Moayyed Kazemi A, Ahmadpour F, Yalameha* B. Correlation of MicroRNA-125b, Sirtuin, and Signal Transducer and Activator of Transcription 3 with Biochemical Parameters and Risk Factors in Atherosclerosis Patients. rbmb.net 2024; 12 (4) :631-642
URL: http://rbmb.net/article-1-1300-en.html
URL: http://rbmb.net/article-1-1300-en.html
Atefeh Mirderikvand 
, Gholamreza Shahsavari , Alireza Moayyed Kazemi , Fatemeh Ahmadpour * , Banafsheh Yalameha*
, Gholamreza Shahsavari , Alireza Moayyed Kazemi , Fatemeh Ahmadpour * , Banafsheh Yalameha*
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.
Abstract: (452 Views)
Background: Atherosclerosis (AS) is an inflammatory disease linked to vascular events, with dysregulation of microRNA (miR)-125b, contributing to cardiovascular disease pathogenesis. Moreover, there is evidence of the involvement of signal transducer and activator of transcription 3 (STAT3) and sirtuin 6 (SIRT6) in AS. This study aimed to survey the expression levels of miR-125b, STAT3, and SIRT6 in the peripheral blood mononuclear cells (PBMCs) of AS patients and controls, and to find their correlations with biochemical parameters and risk factors.
Methods: This study included blood samples from 45 controls and 45 AS patients, with PBMCs isolated using Ficoll solution. Expression levels of miR-125b, STAT3, and SIRT6 were determined via quantitative Real Time-PCR.
Results: The findings revealed a significant increase in miR-125b levels in patients compared to controls (P = 0.017). However, alterations in STAT3 and SIRT6 expression were not significant (P> 0.05). There was no substantial relationship between miR-125b and STAT3 (P = 0.522) or SIRT6 (P = 0.88). miR-125b showed a significant relationship with atherogenic indexes and creatinine (P<0.05), while the association of SIRT6 with HDL and creatinine was significant (P<0.05). STAT3 exhibited high diagnostic power for identifying individuals at risk of heart disease and hypertension (P<0.05).
Conclusions: STAT3 can serve as a valuable biomarker for detecting AS and AS-related risk factors. miR-125b and SIRT6 may be associated with AS lipid metabolism. However, further studies with larger sample sizes are recommended to mechanistically elucidate the association of these genes.
Keywords: Atherosclerosis, MicroRNA-125b, STAT3, SIRT6, Leukocyte.
Methods: This study included blood samples from 45 controls and 45 AS patients, with PBMCs isolated using Ficoll solution. Expression levels of miR-125b, STAT3, and SIRT6 were determined via quantitative Real Time-PCR.
Results: The findings revealed a significant increase in miR-125b levels in patients compared to controls (P = 0.017). However, alterations in STAT3 and SIRT6 expression were not significant (P> 0.05). There was no substantial relationship between miR-125b and STAT3 (P = 0.522) or SIRT6 (P = 0.88). miR-125b showed a significant relationship with atherogenic indexes and creatinine (P<0.05), while the association of SIRT6 with HDL and creatinine was significant (P<0.05). STAT3 exhibited high diagnostic power for identifying individuals at risk of heart disease and hypertension (P<0.05).
Conclusions: STAT3 can serve as a valuable biomarker for detecting AS and AS-related risk factors. miR-125b and SIRT6 may be associated with AS lipid metabolism. However, further studies with larger sample sizes are recommended to mechanistically elucidate the association of these genes.
Keywords: Atherosclerosis, MicroRNA-125b, STAT3, SIRT6, Leukocyte.
Keywords: Atherosclerosis, microRNA-125b, Signal transducer and activator of transcription 3, Sirtuin 6, Leukocyte
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2023/12/8 | Accepted: 2024/04/23 | Published: 2024/07/2
Received: 2023/12/8 | Accepted: 2024/04/23 | Published: 2024/07/2
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