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Volume 13, Issue 2 (Vol.13 No.2 Jul 2024)
rbmb.net 2024, 13(2): 204-217 |
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Ibrahim M M, Osman A, Helal A I, Faheem A M, El-Kattan M A, Ibrahim I, et al . Celastrol Mitigates Acetaminophen-Induced Nephrotoxicity in Rats via Targeting Renal Oxidative Stress, Inflammation, Apoptosis with Enhancement in Aquaporin 1 Level. rbmb.net 2024; 13 (2) :204-217
URL: http://rbmb.net/article-1-1383-en.html
URL: http://rbmb.net/article-1-1383-en.html
Mohie Mahmoud Ibrahim 
, Amira Osman , Azza Ibrahim Helal , Ahmed Mohsen Faheem , Mohammad Abd-El-Same'e El-Kattan , Iman Ibrahim , Ahmed Abdel-monem Elmetwally , Sara Abubakr , Alaa Mohamed Badawy , Emadeldeen Hussin *
, Amira Osman , Azza Ibrahim Helal , Ahmed Mohsen Faheem , Mohammad Abd-El-Same'e El-Kattan , Iman Ibrahim , Ahmed Abdel-monem Elmetwally , Sara Abubakr , Alaa Mohamed Badawy , Emadeldeen Hussin *
Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Egypt.
Abstract: (226 Views)
Background: Acetaminophen also name paracetamol is apopular antipyretic and analgesic drug, in alarge doses produces a cute kidney injury either in human and animals. The aim of this study to assess the effect of celastrol in reducing acetaminophen-induced nephrotoxicity and to elucidate its underlying mechanisms.
Methods: Rats were divided into four groups: control, celastrol-treated, acetaminophen-exposed, and a group receiving both acetaminophen and celastrol. After 24 hours, blood samples were taken and kidney tissues were harvested for histological and molecular analyses. The findings shed light on the protective effects of celastrol against acetaminophen-induced nephrotoxicity, offering insights into its therapeutic potential.
Results: paracetamol oral intake altered renal histology with significantly P< 0.05 increased serum creatinine, blood urea nitrogen (BUN), and homogenate malonaldhyde (MDA), and immunoexpression of tumor necrosis- alpha (TNF-α), interleukin-6 (IL-6), caspase-3, Bcl-2-associated X- protein (Bax). Furthermore, it decreases homogenate level of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and gene expression of nuclear factor erythroid 2–related factor 2 (Nrf2), and haem oxygenase-1 (HO-1). Meanwhile, intraperitoneal injection of celastrol with acetaminophen reaffirms the previous results.
Conclusion: We provided a novel treatment against acetaminophen induced-nephrotoxicity with targeting renal oxidative stress, inflammation, apoptosis with elevation of Aquaporin 1 (AQP1) level.
Methods: Rats were divided into four groups: control, celastrol-treated, acetaminophen-exposed, and a group receiving both acetaminophen and celastrol. After 24 hours, blood samples were taken and kidney tissues were harvested for histological and molecular analyses. The findings shed light on the protective effects of celastrol against acetaminophen-induced nephrotoxicity, offering insights into its therapeutic potential.
Results: paracetamol oral intake altered renal histology with significantly P< 0.05 increased serum creatinine, blood urea nitrogen (BUN), and homogenate malonaldhyde (MDA), and immunoexpression of tumor necrosis- alpha (TNF-α), interleukin-6 (IL-6), caspase-3, Bcl-2-associated X- protein (Bax). Furthermore, it decreases homogenate level of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and gene expression of nuclear factor erythroid 2–related factor 2 (Nrf2), and haem oxygenase-1 (HO-1). Meanwhile, intraperitoneal injection of celastrol with acetaminophen reaffirms the previous results.
Conclusion: We provided a novel treatment against acetaminophen induced-nephrotoxicity with targeting renal oxidative stress, inflammation, apoptosis with elevation of Aquaporin 1 (AQP1) level.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2024/04/28 | Accepted: 2024/06/9 | Published: 2025/01/4
Received: 2024/04/28 | Accepted: 2024/06/9 | Published: 2025/01/4
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