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Volume 13, Issue 3 (Vol.13 No.3 Oct 2024)
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Al-Halfi A S S, Al-Shammari M Z F, Ibrahim S K, Alshareef S A. Effect of Active Compounds of Eruca Sativa Plant Extract on Staphylococcus aureus Bacteria. rbmb.net 2024; 13 (3) :394-404
URL: http://rbmb.net/article-1-1412-en.html
URL: http://rbmb.net/article-1-1412-en.html
Alaa Salim Sadah Al-Halfi * 
, Maher Zaki Faisal Al-Shammari , Suaad Khalil Ibrahim , Sohad Abdulkaleg Alshareef
, Maher Zaki Faisal Al-Shammari , Suaad Khalil Ibrahim , Sohad Abdulkaleg Alshareef
Department of Biology, College of Education for Pure Science/ Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq.
Abstract: (345 Views)
Background: Medicinal plants play an important role in agricultural production due to their therapeutic significance, particularly in the treatment of various pathological conditions.
Methods: Active compounds in Eruca sativa were identified using High-Performance Liquid Chromatography (HPLC). Fifty isolates of Staphylococcus aureus (S. aureus) bacteria were obtained. The effect of Eruca sativa plant extract on biofilm formation of bacterial isolates was tested using the standard plate method.
Results: The highest percentage of S. aureus was found in wound samples, with 22 isolates (44%). The isolates showed variability in their ability to form biofilms. The efficacy test revealed that plants treated with different concentrations of brassinolide (0, 1.5, 2.5, 3.5 mg/L) and Eruca sativa showed inhibition of S. aureus growth in isolates (S2, S7, S10, S15, S16, S31, S42, S48, S50) with alcohol concentrations (5, 25, 75 mg/ml). The effect of Eruca sativa plant extract was dependent on the concentration of brassinolide applied. The highest inhibition was observed with brassinolide concentration of 3.5 mg/L-1 and alcohol extract concentrations of 50 and 75 mg/ml.
Conclusion: The alcoholic extract from the leaves of Eruca sativa, combined with brassinolide, proved effective in inhibiting the growth and biofilm formation of S. aureus bacteria.
Methods: Active compounds in Eruca sativa were identified using High-Performance Liquid Chromatography (HPLC). Fifty isolates of Staphylococcus aureus (S. aureus) bacteria were obtained. The effect of Eruca sativa plant extract on biofilm formation of bacterial isolates was tested using the standard plate method.
Results: The highest percentage of S. aureus was found in wound samples, with 22 isolates (44%). The isolates showed variability in their ability to form biofilms. The efficacy test revealed that plants treated with different concentrations of brassinolide (0, 1.5, 2.5, 3.5 mg/L) and Eruca sativa showed inhibition of S. aureus growth in isolates (S2, S7, S10, S15, S16, S31, S42, S48, S50) with alcohol concentrations (5, 25, 75 mg/ml). The effect of Eruca sativa plant extract was dependent on the concentration of brassinolide applied. The highest inhibition was observed with brassinolide concentration of 3.5 mg/L-1 and alcohol extract concentrations of 50 and 75 mg/ml.
Conclusion: The alcoholic extract from the leaves of Eruca sativa, combined with brassinolide, proved effective in inhibiting the growth and biofilm formation of S. aureus bacteria.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2024/06/18 | Accepted: 2024/08/19 | Published: 2025/04/12
Received: 2024/06/18 | Accepted: 2024/08/19 | Published: 2025/04/12
References
1. Al-Amara SSM. Constitutive and Inducible Clindamycin Resistance Frequencies among Staphylococcus sp. Coagulase Negative Isolates in Al-Basrah Governorate, Iraq. Rep Biochem Mol Biol. 2022;11(1):30-35. [DOI:10.52547/rbmb.11.1.30] [PMID] []
2. Al-Shuwaikh AMA, Al-Shuwaikh RMA, Hassan, JS. Effect of Trigonella foenum Extract and ZiO2 Nanoparticles on Some Pathogenic Fungi and Bacteria. Prensa Médical Argent. 2019; 105(5): 302-308.
3. Shoaib M, Aqib AI, Muzammil I, Majeed N, Bhutta ZA, Kulyar MF, et al. MRSA compendium of epidemiology, transmission, pathophysiology, treatment, and prevention within one health framework. Front Microbiol. 2023;13:1067284. [DOI:10.3389/fmicb.2022.1067284] [PMID] []
4. Ahmed RZT, Alshwaikh RM. Detection of Integron Classes and Agr Group in Staphylococcus aureus Isolated from Different Clinical Samples. Ibn Al-Haitham J Pure Appl Sci. 2024; 37(2): 112-128.
5. Grapsa J, Blauth C, Chandrashekhar YS, Prendergast B, Erb B Jr, et al. Staphylococcus Aureus Infective Endocarditis: JACC Patient Pathways. J Am Coll Cardiol. 2022;79(1):88-99. [DOI:10.1016/j.jacc.2021.10.015] [PMID]
6. Sharma S, Mohler J, Mahajan SD, Schwartz SA, Bruggemann L, Aalinkeel R. Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment. Microorganisms, 2023;11(6):1614. [DOI:10.3390/microorganisms11061614] [PMID] []
7. Mirzaei B, Babaei R, Haghshenas MR, Mohammadi F, Homayoni P, Shafaei E. PIA and rSesC Mixture Arisen Antibodies Could Inhibit the Biofilm-Formation in Staphylococcus aureus. Rep Biochem Mol Biol. 2021;10(1):1-12. [DOI:10.52547/rbmb.10.1.1] [PMID] []
8. Foster TJ. Antibiotic resistance in Staphylococcus aureus. Current status and future prospects. FEMS Microbiol Rev. 2017;41(3):430-449. [DOI:10.1093/femsre/fux007] [PMID]
9. Edo GI, Yousif E, Al-Mashhadani MH. Chitosan: An overview of biological activities, derivatives, properties, and current advancements in biomedical applications. Carbohydr. Res. 2024;542:109199. [DOI:10.1016/j.carres.2024.109199] [PMID]
10. Edo GI, Yousif E, Al-Mashhadani MH. Modified chitosan: Insight on biomedical and industrial applications. Int J Biol Macromol. 2024;275(Pt 1):133526. [DOI:10.1016/j.ijbiomac.2024.133526] [PMID]
11. Sofowora A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med. 2013;10(5):210-29. [DOI:10.4314/ajtcam.v10i5.2] [PMID] []
12. Makia R, Al-Sammarrae K, Al-Halbosiy M, Al-Mashhadani M. In Vitro Cytotoxic Activity of Total Flavonoid from Equisetum Arvense Extract. Rep Biochem Mol Biol. 2022;11(3):487-492. [DOI:10.52547/rbmb.11.3.487] [PMID] []
13. Alo M, Eze UG, Anyim C. In vitro Antimicrobial activities of Extracts of Magnifera indica, Caricapapaya and Psidium guajava Leaves on Samonella typhi isolates. Medicine, Environmental Sci, Biol. 2012; 1: 1-6.
14. Pagnotta E, Ugolini L, Matteo R, Righetti L. Bioactive Compounds from Eruca sativa Seeds. Encyclopedia, 2022; 2(4): 1866-1879. [DOI:10.3390/encyclopedia2040129]
15. Shareef B, Al Qadhi HI, Ahmed SJ, Amran M, Ibrahim ZO. Implementation of Eruca sativa Extract for the Preparation of Nano-Selenium Particles. Al-Rafidain J Med Sci. 2023;5:26-33 [DOI:10.54133/ajms.v5i.141]
16. Morales M, Janick J. Arugula: A Promising Specialty Leaf Vegetable. In: Trends in New Crops and New Use, Eds, Janick J, Whipkey A. ASHS Press: Alexandria, 2002.
17. Sultana B, Anwar F, Ashraf M. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 2009;14(6):2167-80. [DOI:10.3390/molecules14062167] [PMID] []
18. Chen BH, Chuang JR, Lin JH, Chiu CP. Quantification of Provitamin A Compounds in ChInese Vegetables by High-Performance Liquid Chromatography. J Food Prot. 1993;56(1):51-54. [DOI:10.4315/0362-028X-56.1.51] [PMID]
19. AL-Kazaz EJ, Melconian AK, Kandela NJ. Extraction of Staphyloxanthin from Staphylococcus aureus Isolated from Clinical Sources to Determine its Antibacterial Activity Against other Bacteria. Iraqi J Sci. 2023; 55(4B): 1823-1832.
20. Bakhtiari NM, Javadmakoei S. Survey on biofilm production and presence of attachment factors in human uropathogenic strains of Escherichia coli. Jundishapur J Microbil. 2017;10(6): e13108. [DOI:10.5812/jjm-13108] [PMID] []
21. Awadelkareem AM, Al-Shammari E, Elkhalifa AO, Adnan M, Siddiqui AJ, Mahmood D, et al. Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach. Plants (Basel, Switzerland), 2022; 11(5): 610 [DOI:10.3390/plants11050610] [PMID] []
22. Castorina G, Consonni G. The Role of Brassinosteroids in Controlling Plant Height in Poaceae: A Genetic Perspective. Int J Molec Sci. 2020; 21(4): 1191. [DOI:10.3390/ijms21041191] [PMID] []
23. Ding L, Cao J, Lin W, Chen H, Xiong X, Ao H, et al. The Roles of Cyclin-Dependent Kinases in Cell-Cycle Progression and Therapeutic Strategies in Human Breast Cancer. Int J Molec Sci. 2020; 21(6):1960. [DOI:10.3390/ijms21061960] [PMID] []
24. Omar NN, Mohammed RK. A Molecular Study of Toxic Shock Syndrome Toxin gene (tsst-1) in β-lactam Resistant Staphylococcus aureus Clinical Isolates. Iraqi J Sci. 2021;62(3): 825-837. [DOI:10.24996/ijs.2021.62.3.13]
25. Hameed A, Al-Wandawy SH, Zwain LA, Wandawy A. Ability of Staphylococcus spp. Isolated from Meningitis Patients to Biofilm Formation. Indian J Forensic Med & Toxicol. 2020,14(4):1585.
26. Al-Wandawy AH, Zwain LA, Omer SA. Antibacterial and antibiofilm effect of menthol and thymol on vaginal bacteria. Biochem Cell Arch. 2020; 20(Sup. 2): 3883-3888.
27. Rasool LM. Prevalence of bacteremia among children complaining different kinds of infections under 12 years old in Baghdad. Baghdad Sci J. 2011; 8(2): 280-285.
https://doi.org/10.21123/bsj.2011.8.2.280-285 [DOI:10.21123/bsj.8.2.280-285]
28. Al-Musawi ET, Aljobori KM, Jaber HJK. Antibiofilm Activity of Chalcone in Methicilin Resistant Staphylococcus aureus. Iraqi J. Biotechnol. 2019;18(3): 62-71.
29. Mohammed SW, Radif HM. Detection of icaA Gene Expression in Clinical Biofilm-Producing Staphylococcus aureus Isolates. Iraqi J Sci. 2020;61(12): 3154-3163. [DOI:10.24996/ijs.2020.61.12.2]
30. Lade H, Park JH, Chung SH, Kim IH, Kim JM, Joo HS, Kim JS. Biofilm Formation by Staphylococcus aureus Clinical Isolates is Differentially Affected by Glucose and Sodium Chloride Supplemented Culture Media. J Clin Med. 2019; 8(11), 1853. [DOI:10.3390/jcm8111853] [PMID] []
31. Al Ani ATA, Al Meani SAL. Molecular screening of adhesion proteins genes in Staphylococcus aureus strains isolated from different clinical infections in Baghdad city and identification of their relationship with some virulence factors. Al-Nahrain J Sci. 2018; 21(1):79-89. [DOI:10.22401/JNUS.21.1.13]
32. de Souza GC, Haas AP, von Poser GL, Schapoval EE, Elisabetsky E. Ethnopharmacological studies of antimicrobial remedies in the south of Brazil. J Ethnopharmacol. 2004;90(1):135-43.
https://doi.org/10.1016/j.jep.2003.09.039 [DOI:10.1016/j.jep.2011.03.003] [PMID]
33. Ali EH, Hussein AA. The Effect of Eruca sativa extract on Gram Posative and Negative Bacteria. Baghdad Sci J 2007; 4(3): 375-378.
https://doi.org/10.21123/bsj.4.3.375-378 [DOI:10.21123/bsj.2007.4.3.375-378]
34. Ali IH, Hussein AA. The effectiveness of watercress extract (Eruca sativa) as an antibacterial agent against Gram-positive and Gram-negative bacteria. Baghdad Sci J. 2007; 4(3): 375-378.
https://doi.org/10.21123/bsj.4.3.375-378 [DOI:10.21123/bsj.2007.4.3.375-378]
35. Hussain MH, Rabie KM, Hassoon AS. Effect of extraction method and plant part on Eruca sativa L. content from some alkaloids compounds. Biochem Cell Arch. 2020; 20(1): 719-722.
36. Kyung KH, Lee YC. Antimicrobial activity of sulfer compound derived from some S-Alkyl-systeine sulfoxides and Allium and Brassica. Food Rev. Int. 2001; 17(2): 183-198. [DOI:10.1081/FRI-100000268]
37. Yan Y, Li X, Zhang C, Lv L, Gao B, Li M. Research Progress on Antibacterial Activities and Mechanisms of Natural Alkaloids: A Review. Antibiotics (Basel, Switzerland), 2-21; 10(3): 318.
https://doi.org/10.3390/antibiotics10030318 [DOI:10.3390/antibiotics10030318.] [PMID] []
38. Tatli Cankaya II, Somuncuoglu EI. Potential and Prophylactic Use of Plants Containing Saponin-Type Compounds as Antibiofilm Agents against Respiratory Tract Infections. Evid-based Complem Altern Med: eCAM, 2021; 6814215. [DOI:10.1155/2021/6814215] [PMID] []
39. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82. [DOI:10.1128/CMR.12.4.564] [PMID] []
40. Chmur M, Bajguz A. Brassinolide Enhances the Level of Brassinosteroids, Protein, Pigments, and Monosaccharides in Wolffia arrhiza Treated with Brassinazole. Plants (Basel, Switzerland), 2021;10(7): 1311 [DOI:10.3390/plants10071311] [PMID] []
41. Abas HM, Ahmed MF. The effect of some amino acids on the biofilm of bacteria Staphylococcus aureus. Diyala J Agricultural Sci. 2014, 6(2): 27-38.
42. Lam H, Oh DC, Cava F, Takacs CN, Clardy J, de Pedro MA, Waldor MK. D-Amino Acids Govern Stationary Phase Cell Wall Remodeling in Bacteria. Science. 2009; 325(5947):1552-5. [DOI:10.1126/science.1178123] [PMID] []
43. Hochbaum AI, Kolodkin-Gal I, Foulston L, Kolter R, Aizenberg J, Losick R. Inhibitory effects of D-amino acids on Staphylococcus aureus biofilm development. J Bacteriol. 2011; 193(20):5616-22. [DOI:10.1128/JB.05534-11] [PMID] []
44. Peng, Q., Tang, X., Dong, W., Sun, N., & Yuan, W. (2022). A Review of Biofilm Formation of Staphylococcus aureus and Its Regulation Mechanism. Antibiotics (Basel, Switzerland), 12(1), 12. [DOI:10.3390/antibiotics12010012] [PMID] []
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