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Volume 13, Issue 3 (Vol.13 No.3 Oct 2024)
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Pradana A, Sari D K, Rusda M, Tarigan A P, Wiyono W H, Soeroso N N, et al . Protective Effects of Probiotics Against Systemic Inflammation in Mice Model with Chronic Obstructive Pulmonary Disease Induced by Cigarette-smoke. rbmb.net 2024; 13 (3) :322-328
URL: http://rbmb.net/article-1-1427-en.html
URL: http://rbmb.net/article-1-1427-en.html
Andika Pradana * 
, Dina Keumala Sari , Muhammad Rusda , Amira Permatasari Tarigan , Wiwien Heru Wiyono , Noni Novisari Soeroso , Putri Chairani Eyanoer , Mustafa Mahmud Amin
, Dina Keumala Sari , Muhammad Rusda , Amira Permatasari Tarigan , Wiwien Heru Wiyono , Noni Novisari Soeroso , Putri Chairani Eyanoer , Mustafa Mahmud Amin
Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia & Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.
Abstract: (672 Views)
Background: Systemic inflammation is one of hallmarks in chronic obstructive pulmonary disease (COPD), contributing to high morbidity and mortality due to elevated levels of interleukin-6 (IL-6) and reduced level of interleukin-10 (IL-10). Probiotics have the potential to reduce systemic inflammation through the gut-lung axis. This study aims to assess the effect of probiotics compared with an inhaled bronchodilator on serum IL-6 and IL-10 levels in mice model of COPD.
Methods: This was an in vivo experimental study with a post-test only control group design. Thirty C57BL/6 mice were randomized into five groups; NC (healthy mice), PC (COPD induced mice); T1 (COPD mice treated with a bronchodilator), T2 (COPD mice treated with probiotics) and T3 (COPD mice treated with both a bronchodilator and probiotics). COPD was induced for 12 weeks, followed by a 6-week treatment period. After completing the treatment, serum IL-6 and IL-10 levels were measured using the enzyme-linked immunosorbent assay (ELISA).
Results: The IL-6 levels in T2 group were reduced to levels comparable to the negative control group (13.5 vs 12.0 pg/ml respectively, p=0.84). The IL-10 levels were higher in T2 group compared to T1 group, however; this difference was not statistically significant (181.4 vs 155.0 respectively, p>0.05).
Conclusion: In mice model of COPD, probiotics have been shown to lower IL-6 levels and, to a lesser extent, increased IL-10. As a result, probiotics may have a protective effect against systemic inflammation.
Methods: This was an in vivo experimental study with a post-test only control group design. Thirty C57BL/6 mice were randomized into five groups; NC (healthy mice), PC (COPD induced mice); T1 (COPD mice treated with a bronchodilator), T2 (COPD mice treated with probiotics) and T3 (COPD mice treated with both a bronchodilator and probiotics). COPD was induced for 12 weeks, followed by a 6-week treatment period. After completing the treatment, serum IL-6 and IL-10 levels were measured using the enzyme-linked immunosorbent assay (ELISA).
Results: The IL-6 levels in T2 group were reduced to levels comparable to the negative control group (13.5 vs 12.0 pg/ml respectively, p=0.84). The IL-10 levels were higher in T2 group compared to T1 group, however; this difference was not statistically significant (181.4 vs 155.0 respectively, p>0.05).
Conclusion: In mice model of COPD, probiotics have been shown to lower IL-6 levels and, to a lesser extent, increased IL-10. As a result, probiotics may have a protective effect against systemic inflammation.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2024/07/9 | Accepted: 2024/12/25 | Published: 2025/04/12
Received: 2024/07/9 | Accepted: 2024/12/25 | Published: 2025/04/12
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