Volume 9, Issue 4 (Vol.9 No.4 Jan 2021)                   rbmb.net 2021, 9(4): 373-378 | Back to browse issues page

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Shahabinejad M, zare R, Asadi Z, Mohajertehran F. LAMP3 (CD208) Expression in Squamous Cell Carcinoma and Epithelial Dysplasia of the Oral Cavity and Clinicopathological Characteristics of Unfavorable Prognosis. rbmb.net 2021; 9 (4) :373-378
URL: http://rbmb.net/article-1-582-en.html
Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran & Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (2963 Views)
Background: This study aimed to evaluate LAMP3 (CD208) gene expression in oral squamous cell carcinoma (OSCC) and dysplastic oral epithelium by quantitative real-time polymerase chain reaction (qPCR) and compare LAMP3 expression in different disease grades and stages.

Methods: In this study, 60 OSCC and dysplastic oral epithelium samples were obtained from the Mashhad University of Medical Sciences together with their demographic and clinicopathological documents. LAMP3 expression was measured by qPCR.

Results: LAMP3 expression was significantly greater in OSCC than in dysplasia samples (P=0.001), in grade III OSCC than in grades I and II, and also greater in advanced than in early OSCC disease stage (P=0.001).

Conclusions: The significantly greater LAMP3 expression in OSCC than in dysplastic epithelium indicates a role for LAMP3 in carcinogenesis in oral mucosa. Our results suggest LAMP3 may be useful as an anticancer target and/or to predict disease pathogenesis in OSCC patient’s cells.
Full-Text [PDF 367 kb]   (767 Downloads)    
Type of Article: Original Article | Subject: Molecular Biology
Received: 2020/10/2 | Accepted: 2020/10/19 | Published: 2021/03/8

1. Neville BW, Damm D, Allen C, Bouquot J. Salivary Gland Pathology. Oral and Maxillofacial Pathology. Elsevier, St. Louis; 2009.
2. Regezi JA, Sciubba J, Jordan RC. Oral pathology: clinical pathologic correlations: Elsevier Health Sciences; 2016.
3. Pektaș ZÖ, Keskin A, Günhan Ö, Karslioğlu Y. Evaluation of nuclear morphometry and DNA ploidy status for detection of malignant and premalignant oral lesions: quantitative cytologic assessment and review of methods for cytomorphometric measurements. Journal of oral and maxillofacial surgery. 2006;64(4):628-35. [DOI:10.1016/j.joms.2005.12.010] [PMID]
4. Marshall JR, Graham S, Haughey BP, Shedd D, O'Shea R, Brasure J, et al. Smoking, alcohol, dentition and diet in the epidemiology of oral cancer. European Journal of Cancer Part B: Oral Oncology. 1992;28(1):9-15. [DOI:10.1016/0964-1955(92)90005-L]
5. Hindle I, Downer M, Speight P. The epidemiology of oral cancer. British Journal of Oral and Maxillofacial Surgery. 1996;34(5):471-6. [DOI:10.1016/S0266-4356(96)90111-4]
6. Lu J, Ma H, Lian S, Huang D, Lian M, Zhang Y, et al. Clinical significance and prognostic value of the expression of LAMP3 in oral squamous cell carcinoma. Disease markers. 2017;2017. [DOI:10.1155/2017/1218254] [PMID] [PMCID]
7. Liao X, Chen Y, Liu D, Li F, Li X, Jia W. High expression of LAMP3 is a novel biomarker of poor prognosis in patients with esophageal squamous cell carcinoma. International journal of molecular sciences. 2015;16(8):17655-67. [DOI:10.3390/ijms160817655] [PMID] [PMCID]
8. Zhao H, Zhu H, Jin Q, Zhang S, Wang W, Wang D, et al. Association of high expression of Groβ with clinical and pathological characteristics of unfavorable prognosis in gastrointestinal stromal tumors. Disease markers. 2015;2015. [DOI:10.1155/2015/171035] [PMID] [PMCID]
9. Nagelkerke A, Mujcic H, Bussink J, Wouters BG, van Laarhoven HW, Sweep FC, et al. Hypoxic regulation and prognostic value of LAMP3 expression in breast cancer. Cancer. 2011;117(16):3670-81. [DOI:10.1002/cncr.25938] [PMID]
10. Nagelkerke A, Sieuwerts AM, Bussink J, Sweep F, Look MP, Foekens JA, et al. LAMP3 is involved in tamoxifen resistance in breast cancer cells through the modulation of autophagy. Endocr Relat Cancer. 2014;21(1):101-12. [DOI:10.1530/ERC-13-0183] [PMID]
11. Kanao H, Enomoto T, Kimura T, Fujita M, Nakashima R, Ueda Y, et al. Overexpression of LAMP3/TSC403/DC-LAMP promotes metastasis in uterine cervical cancer. Cancer research. 2005;65(19):8640-5. [DOI:10.1158/0008-5472.CAN-04-4112] [PMID]
12. Nagelkerke A, Bussink J, Mujcic H, Wouters BG, Lehmann S, Sweep FC, et al. Hypoxia stimulates migration of breast cancer cells via the PERK/ATF4/LAMP3-arm of the unfolded protein response. Breast Cancer Research. 2013;15(1):R2. [DOI:10.1186/bcr3373] [PMID] [PMCID]
13. Peltanova B, Raudenska M, Masarik M. Effect of tumor microenvironment on pathogenesis of the head and neck squamous cell carcinoma: a systematic review. Molecular cancer. 2019;18(1):63. [DOI:10.1186/s12943-019-0983-5] [PMID] [PMCID]
14. Mohtasham N, Ayatollahi H, Saghravanian N, Zare R, Shakeri M-T, Sahebkar A, et al. Evaluation of Tissue and Serum Expression Levels of Lactate Dehydrogenase Isoenzymes in Patients with Head and Neck Squamous Cell Carcinoma. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents).2019;19(17):2072-8. [DOI:10.2174/1871520619666191014160818] [PMID]
15. Mohajertehran F, Ayatollahi H, Jafarian AH, Khazaeni K, Soukhtanloo M, Shakeri M-T, et al. Overexpression of lactate dehydrogenase in the saliva and tissues of patients with head and neck squamous cell carcinoma. Reports of biochemistry & molecular biology. 2019;7(2):142.
16. Mohajertehran F, Ayatollahi H, Khazaeni K, Shakeri M-T, Mohtasham N. Overexpression of high-mobility motor box 1 in the blood and tissues of patients with head and neck squamous cell carcinoma. Iranian journal of otorhinolaryngology. 2018;30(100):261.
17. Fennelly C, Amaravadi RK. Lysosomal biology in cancer. Lysosomes: Springer; 2017. p. 293-308. [DOI:10.1007/978-1-4939-6934-0_19] [PMID] [PMCID]
18. Pu J, Guardia CM, Keren-Kaplan T, Bonifacino JS. Mechanisms and functions of lysosome positioning. Journal of cell science. 2016;129(23):4329-39. [DOI:10.1242/jcs.196287] [PMID] [PMCID]
19. Alessandrini F, Pezzè L, Ciribilli Y, editors. LAMPs: Shedding light on cancer biology. Seminars in oncology; 2017: Elsevier. [DOI:10.1053/j.seminoncol.2017.10.013] [PMID]
20. Grade M, Difilippantonio MJ, Camps J. Patterns of chromosomal aberrations in solid tumors. Chromosomal instability in Cancer cells: Springer; 2015. p. 115-42. [DOI:10.1007/978-3-319-20291-4_6] [PMID] [PMCID]
21. Qiu X, You Y, Huang J, Wang X, Zhu H, Wang Z. LAMP3 and TP53 overexpression predicts poor outcome in laryngeal squamous cell carcinoma. International journal of clinical and experimental pathology. 2015;8(5):5519.

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