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Jafarzadeh Samani R, Tajbakhsh E, Momtaz H, Kabiri Samani M. Prevalence of Virulence Genes and Antibiotic Resistance Pattern in Enterococcus Faecalis Isolated from Urinary Tract Infection in Shahrekord, Iran. rbmb.net 2021; 10 (1) :50-59
URL: http://rbmb.net/article-1-593-en.html
URL: http://rbmb.net/article-1-593-en.html
Department of Microbiology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Abstract: (2958 Views)
Background: This study aims to specify the antimicrobial resistance pattern and virulence genes of Enterococcus faecalis isolated from urinary tract infections in Shahrekord, Iran.
Methods: Urine samples of 1000 people suspected of having urinary tract infections referred to Shahrekord medical diagnostic laboratories were examined. Biofilm assays were performed by microtiter plate test through reading the OD490. Polymerase Chain Reaction (PCR) was applied to study the virulence factors.
Results: Enterococcus faecalis was detected in 60 samples. After performing microbiological tests, all samples were positive in the molecular analysis. Strong, moderate and weak biofilm reactions reported 66.67%, 25%, and 8.33% respectively. The most resistance reported to cotrimoxazole, vancomycin and amikacin and the lowest resistance to nitrofurantoin (8.33%) was reported. Statistical analysis with Fisher's exact test showed a statistically significant relationship between biofilm production and resistance to cotrimoxazole, vancomycin and cefotaxime. Prevalence of efe A, ace, gel E, esp, cyl M, agg, cyl A and cyl B in strong biofilm formation isolates was reported 100%, 87.5%, 82%, 62.5%, 55%, 37.5% 25% and 22.5% respectively. There was a significant relationship between the frequency of efa A and strong biofilm reaction.
Conclusions: The presence of E. faecalis strains resistant to co-trimoxazole and vancomycin and present of some virulence factors is alarming the researchers. Since antibiotic resistance genes are probably transmitted among enterococci, and Staphylococci, controlling infections made by enterococci as well as the appropriate administration of antibiotics could treat the nosocomial infections effectively.
Methods: Urine samples of 1000 people suspected of having urinary tract infections referred to Shahrekord medical diagnostic laboratories were examined. Biofilm assays were performed by microtiter plate test through reading the OD490. Polymerase Chain Reaction (PCR) was applied to study the virulence factors.
Results: Enterococcus faecalis was detected in 60 samples. After performing microbiological tests, all samples were positive in the molecular analysis. Strong, moderate and weak biofilm reactions reported 66.67%, 25%, and 8.33% respectively. The most resistance reported to cotrimoxazole, vancomycin and amikacin and the lowest resistance to nitrofurantoin (8.33%) was reported. Statistical analysis with Fisher's exact test showed a statistically significant relationship between biofilm production and resistance to cotrimoxazole, vancomycin and cefotaxime. Prevalence of efe A, ace, gel E, esp, cyl M, agg, cyl A and cyl B in strong biofilm formation isolates was reported 100%, 87.5%, 82%, 62.5%, 55%, 37.5% 25% and 22.5% respectively. There was a significant relationship between the frequency of efa A and strong biofilm reaction.
Conclusions: The presence of E. faecalis strains resistant to co-trimoxazole and vancomycin and present of some virulence factors is alarming the researchers. Since antibiotic resistance genes are probably transmitted among enterococci, and Staphylococci, controlling infections made by enterococci as well as the appropriate administration of antibiotics could treat the nosocomial infections effectively.
Type of Article: Original Article |
Subject:
Microbiology
Received: 2020/10/31 | Accepted: 2020/12/13 | Published: 2021/05/9
Received: 2020/10/31 | Accepted: 2020/12/13 | Published: 2021/05/9
References
1. Behshood P, Tajbakhsh E, Momtaz H. Recognition of (Sesc) for easy identification of Staphylococcus epidermidis and molecular and phenotypic study of Β-Lactam persistence in Staphylococcus epidermidis isolates in Isfahan. Rep Biochem Mol Biol. 2020;9(3):309-314. [DOI:10.29252/rbmb.9.3.309] [PMID] [PMCID]
2. Shahmoradi M, Faridifar P, Shapouri R, Mousavi S F, Ezzedin M, Mirzaei B. Determining the biofilm forming gene profile of Staphylococcus aureus clinical isolates via multiplex colony PCR method. Rep Biochem Mol Biol. 2019;7(2):181-188.
3. Giacometti A, Cirioni O, Schimizzi AM, Del Prete MS, Barchiesi F, D'Errico MM, et al. Epidemiology and microbiology of surgical wound infections. J Clin Microbiol. 2000;38(2):918-22. [DOI:10.1128/JCM.38.2.918-922.2000] [PMID] [PMCID]
4. Falk PS, Winnike J, Woodmansee C, Desai M, Mayhall CG. Outbreak of vancomycin-resistant enterococci in a burn unit. Infect Control Hosp Epidemiol. 2000;21(9):575-82. [DOI:10.1086/501806] [PMID]
5. Suppli M, Aabenhus R, Harboe ZB, Andersen LP, Tvede M, Jensen JU. Mortality in enterococcal bloodstream infections increases with inappropriate antimicrobial therapy. Clin Microbiol Infect. 2011;17(7):1078-83. [DOI:10.1111/j.1469-0691.2010.03394.x] [PMID]
6. McDonald JR, Olaison L, Anderson DJ, Hoen B, et al. Enterococcal endocarditis: 107 cases from the international collaboration on endocarditis merged database. Am J Med. 2005;118(7):759-66. [DOI:10.1016/j.amjmed.2005.02.020] [PMID]
7. Khardori N, Wong E, Carrasco CH, Wallace S, Patt Y, Bodey GP. Infections associated with biliary drainage procedures in patients with cancer. Rev Infect Dis. 1991;13(4):587-91. [DOI:10.1093/clinids/13.4.587] [PMID]
8. Pérez-Fontán M, Rodríguez-Carmona A, Rodríguez-Mayo M. Enterococcal peritonitis in peritoneal dialysis patients: last name matters. Perit Dial Int. 2011;31(5):513-7. [DOI:10.3747/pdi.2011.00022] [PMID]
9. Portillo A, Ruiz-Larrea F, Zarazaga M, Alonso A, Martinez JL, Torres C. Macrolide resistance genes in Enterococcus spp. Antimicrob Agents Chemother. 2000;44(4):967-971. [DOI:10.1128/AAC.44.4.967-971.2000] [PMID] [PMCID]
10. Tambyah PA, Marx JA, Maki DG. Nosocomial infection with vancomycin-dependent enterococci. Emerg Infect Dis. 2004;10(7):1277-81. [DOI:10.3201/eid1007.030993] [PMID] [PMCID]
11. Owens CD, Stoessel K. Surgical site infections: epidemiology, microbiology and prevention. J Hosp Infect. 2008; Suppl 2:3-10. [DOI:10.1016/S0195-6701(08)60017-1]
12. William Costerton J. Cystic fibrosis pathogenesis and the role of biofilms in persistent infection. Trends Microbiol. 2001;9(2):50-2. [DOI:10.1016/S0966-842X(00)01918-1]
13. Moniri R, Ghasemi A, Moosavi S G A, Dastehgoli K, Rezaei M. Virulence gene's relationship with biofilm formation and Detection of aac (6')/aph (2") in Enterococcus faecalis isolated from Patients with Urinary Tract Infection, Jundishapur J Microbiol. 2013; 6(5):e94137. [DOI:10.5812/jjm.6244]
14. Moellering JRC. Emergence of Enterococcus as a significant pathogen. Clin Infect Dis. 1992;14(6):1173-6. [DOI:10.1093/clinids/14.6.1173] [PMID]
15. Shah L, Mulla S, Patel GP, Rewadiwala S. Prevalence of enterococci with higher resistance level in a tertiary care hospital: a matter of concern. Natl J Med Res. 2012;2(1):25-27 [DOI:10.4103/2249-4847.96753] [PMID] [PMCID]
16. Toledo-Arana A, Valle J, Solano C, Arrizubieta MJ, Cucarella C, Lamata M, et al. The enterococcal surface protein, esp, is involved in Enterococcus faecalis biofilm formation. Appl Environ Microbiol. 2001;67(10)4538-45. [DOI:10.1128/AEM.67.10.4538-4545.2001] [PMID] [PMCID]
17. Kayaoglu G, Orstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit Rev Oral Biol Med. 2004;15(5):308-20. [DOI:10.1177/154411130401500506] [PMID]
18. Cetinkaya Y, Falk P, Mayhall CG. Vancomycin-resistant enterococci. Clin Microbiol Rev. 2000;13(4):686-707. [DOI:10.1128/CMR.13.4.686] [PMID] [PMCID]
19. Dunny G, Funk C, Adsit J. Direct stimulation of the transfer of antibiotic resistance by sex pheromones in Streptococcus faecalis. Plasmid. 1981;6(3):270-8. [DOI:10.1016/0147-619X(81)90035-4]
20. Kristich C.J, Li Y.H, Cvitkovitch D.G, Dunny G.M. Esp-Independent Biofilm formation by Enterococcus faecalis. J Bacteriol. 2004;186(1):154-63. [DOI:10.1128/JB.186.1.154-163.2004] [PMID] [PMCID]
21. Li YH, Tang n, Aspiras MB, Lau PC, Lee LH, et al. A quorum-sensing signaling system essential for genetic competence in Streptococcus mutansis involved in biofilm formation. J Bacteriol. 2002;184(10):2699-708. [DOI:10.1128/JB.184.10.2699-2708.2002] [PMID] [PMCID]
22. Loo CY, Corliss DA, Ganeshkumar N. Streptococcus gordoniibiofilm formation: identification of genes that code for biofilm phenotypes. J Bacteriol. 2000;182(5):1374-82. [DOI:10.1128/JB.182.5.1374-1382.2000] [PMID] [PMCID]
23. Kiruthiga A, Padmavathy K, Shabana P, Naveenkumar V, Gnanadesikan S, Malayan J. Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci. BMC Res Notes. 2020;13(1):170. [DOI:10.1186/s13104-020-05018-0] [PMID] [PMCID]
24. Clinical and Laboratory Standards Institute. 2015. Performance standards for antimicrobial susceptibility testing; 23rd informational supplement. CLSI M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA.
25. Sambrook J, Russell DW. Molecular cloning. Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, Cold Spring Harbor. 58-152.
26. Hassan MM, El-Sayed BB. Antibiotic resistance and virulence genes in Enterococcus strains isolated from different hospitals in Saudi Arabia. Biotechnology & Biotechnological Equipment. 2016;30(4):726-732. [DOI:10.1080/13102818.2016.1184992]
27. Barbosa J, Gibbs P, Teixeira P. Virulence factors among enterococci isolated from traditional fermented meat products produced in the North of Portugal. Food Control. 2010;21(5):651-656. [DOI:10.1016/j.foodcont.2009.10.002]
28. Semedo T, Santos MA, Lopes MF, Marques JF, Crespo MT, Tenreiro R. Virulence factors in food, clinical and reference enterococci: a common trait in the genus?. Syst Appl Microbiol. 2003;26(1):13-22 [DOI:10.1078/072320203322337263] [PMID]
29. Silverman J, Thal LA, Perri MB, Bostic G, Zervos MJ. Epidemiologic evaluation of antimicrobial resistance in community-acquired enterococci. J Clin Microbiol. 1998;36(3):830-2. [DOI:10.1128/JCM.36.3.830-832.1998] [PMID] [PMCID]
30. Arias CA, Murray BE. Emergence and management of drug-resistant Enterococcal infections. Expert Rev Anti Infect Ther. 2008;6(5):637-55. [DOI:10.1586/14787210.6.5.637] [PMID]
31. Robert C, Moellering JR. Enterococcus species, Streptococcus bovis and Leuconostoc species. Principles and practice of infectious diseases. 6th ed. New York: Churchill Livingstone; 2005. p. 2411-21.
32. Bereket W, Hemalatha K, Getenet B, Wondwossen T, Solomon A, Zeynudin A, et al. Update on bacterial nosocomial infections. Eur Rev Med Pharmacol Sci. 2012;16(8):1039-44.
33. Kuhn I, Iversen I, Finn M, Greko C, Burman LG, Blanch AR, et al. Occurrence and relatedness of vancomycin-resistant enterococci in animals, Humans, and the environment in different European regions. Appl Environ Microbiol. 2005;71(9):5383-5390. [DOI:10.1128/AEM.71.9.5383-5390.2005] [PMID] [PMCID]
34. Shokoohizadeh L, Ekrami A, Labibzadeh M, Liaqat A, Alavi SM. Antimicrobial resistance patterns and virulence factors of enterococci isolates in hospitalized burn patients. BMC Res Notes. 2018;11:1. [DOI:10.1186/s13104-017-3088-5] [PMID] [PMCID]
35. Joghatei M, Yavarmanesh M, Edalatian Dovom MR. Safety evaluation and antibacterial activity of enterococci isolated from Lighvan cheese. J Food Saf. 2017;37(1). [DOI:10.1111/jfs.12289]
36. Talebi M, Asghari Moghadam N, Mamooii Z, Enayati M, Saifi M, Pourshafie MR. Antibiotic resistance and biofilm formation of Enterococcus faecalis in patient and environmental samples. Jundishapur J Microbiol. 2015;8(10):e23349. [DOI:10.5812/jjm.23349]
37. Gozalan A, Coskun-Ari FF, Ozdem B, Unaldi O, Celikbilek N, Kirca F, et al. Molecular characterization of vancomycin-resistant 39. Enterococcus faecium strains isolated from carriage and clinical samples in a tertiary hospital, Turkey. Journal of Medical Microbiology. 2015;64(7):759-766. [DOI:10.1099/jmm.0.000088] [PMID]
38. Seno y, Kariyama R, Mitsuhata R, Monden K, Kumon H. Clinical implications of biofilm formation by Entrococcus faecalis in the urinary tract. Acta Med Okayama. 2005;59(3):79-87.
39. Heikens E, Leendertse M, Wijnands LM, van Luit-Asbroek M, Bonten MJ, van der Poll T, et al. Enterococcal surface protein Esp is not essential for cell adhesion and intestinal colonization of Enterococcus faecium in mice. BMC Microbiol. 2009;9:19. [DOI:10.1186/1471-2180-9-19] [PMID] [PMCID]
40. Zhengv Jx, Bai B, Lin Zw, Pu Zy, Yao Wm, Chen Z, et al. Characterization of biofilm formation by Enterococcus faecalis isolates derived from urinary tract infections in China. J Med Microbiol. 2018;67(1):60-67. [DOI:10.1099/jmm.0.000647] [PMID] [PMCID]
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