Volume 10, Issue 1 (Vol.10 No.1 Apr 2021)                   rbmb.net 2021, 10(1): 95-104 | Back to browse issues page

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Arghavan B, Shafiee M, Hashemi S J, Khodavaisy S, Hosseinkhan N, Didehdar M, et al . Dysregulation of Key Proteinases in Aspergillus fumigatus Induced by Blood Platelets. rbmb.net. 2021; 10 (1) :95-104
URL: http://rbmb.net/article-1-611-en.html
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Abstract:   (919 Views)
Background: Aspergillus fumigatus is the most common species causing invasive aspergillosis (IA), a life-threatening infection with more than 80% mortality. Interactions between A. fumigatus and human blood platelets lead to intravascular thrombosis and localized infarcts. To better understand A. fumigatus pathogenesis, we aimed to analyze the genetic basis of interactions between the pathogen and blood platelets.

Methods: A bioinformatic pipeline on microarray gene expression dataset, including analysis of differentially expressed genes (DEGs) using Limma R package and their molecular function, as well as biological pathways identification, was conducted to find the effective genes involved in IA. In the wet phase, the gene expression patterns following fungal exposure to blood platelets at 15, 30, 60, and 180 min were evaluated by quantitative reverse transcriptase-PCR analysis.

Results: Three genes encoding aspartic endopeptidases including (Pep1), (Asp f 13), and (β-glucanase) were the standing candidates. The invasion-promoting fungal proteinase-encoding genes were down-regulated after 30 min of hyphal incubation with blood platelets, and then up-regulated at 60 and 180 min, although only Pep1 was greater than the control at the 60and 180 min time points. Also, the same genes were downregulated in more the clinical isolates relative to the standard strain CBS 144.89.

Conclusions: Our findings delineate the possible induction of fungal-encoded proteinases by blood platelets. This provides a new research line into A. fumigatus’ molecular pathogenesis. Such insight into IA pathogenesis might also guide researchers toward novel platelet-based therapies that involve molecular interventions, especially in IA patients.
Full-Text [PDF 540 kb]   (311 Downloads)    
Type of Article: Original Article | Subject: Microbiology
Received: 2020/11/25 | Accepted: 2020/12/13 | Published: 2021/05/9

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