Volume 10, Issue 1 (Vol.10 No.1 Apr 2021)                   rbmb.net 2021, 10(1): 126-134 | Back to browse issues page


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Shahabinejad M, Zare R, Mohajertehran* F, Amouzad Mahdiraji A. Cytokeratins (CK7 and CK20 ) Genes Expression Association with Clinicopathological Indices in Oral Squamous Cell Carcinoma and Dysplastic Oral Epithelium. rbmb.net. 2021; 10 (1) :126-134
URL: http://rbmb.net/article-1-618-en.html
Oral and Maxillofacial Diseases Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract:   (483 Views)
Background: High prevalence of oral squamous cell carcinoma (OSCC) demands the additional novel biological markers. Due to the established roles of cytokeratin in the prognosis of metastasis evaluation the relation of expression of both CK7 and CK20 in OSCC compared to the dysplastic oral epithelium biopsies with clinicopathological factors were investigated.

Methods: We examined the coordinate mRNA expression of cytokeratin 7 (CK7) and cytokeratin 20 (CK20) using quantitative reverse transcription polymerase chain reaction (qRT-PCR) in 110 biopsies of oral squamous epithelium samples including 72 tumoral and 38 dysplastic biopsies. We also collected demographic and pathological data including tumor stage and grade from our patients.

Results: There was a significant difference in CK7 and CK20 gene expression between OSCC and dysplastic samples (p< 0.001). Further, their mean expression in OSCC samples was significantly higher compared to dysplastic samples. Relative mRNA levels of CK7 and CK20 showed that their mean expression in OSCC grade I was significantly lower than other grades (p< 0.01). The relationship between CK7 and CK20 mRNA expression and age or gender was not significant (p> 0.05). Samples in the advanced stage of disease had significantly higher CK7 and CK20 expression compared to early-stage samples of OSCC specimens (p= 0.001).

Conclusions: We found an increase in CK7 and CK20 mRNA levels in grade III OSCC samples compared to other grades. This finding suggests a potential role for CK7 and CK20 in oral mucosal carcinogenesis and OSCC prognosis.
Full-Text [PDF 433 kb]   (173 Downloads)    
Type of Article: Original Article | Subject: Molecular Biology
Received: 2020/11/30 | Accepted: 2020/12/19 | Published: 2021/05/9

References
1. Shahabinejad M, Asadi Z, Mohajertehran F. LAMP3 (CD208) Expression in Squamous Cell Carcinoma and Epithelial Dysplasia of the Oral Cavity and Clinicopathological Characteristics of Unfavorable Prognosis. Reports of Biochemistry and Molecular Biology. 2021;9 (4):373-378. [DOI:10.52547/rbmb.9.4.373]
2. Geum D-H, Roh Y-C, Yoon S-Y, Kim H-G, Lee J-H, Song J-M, et al. The impact factors on 5-year survival rate in patients operated with oral cancer. J Korean Assoc Oral Maxillofac Surg. 2013;39(5):207-216. [DOI:10.5125/jkaoms.2013.39.5.207] [PMID] [PMCID]
3. Liu SA, Wong YK, Lin JC, Poon CK, Tung KC, Tsai WC. Impact of recurrence interval on survival of oral cavity squamous cell carcinoma patients after local relapse. Otolaryngology-Head and Neck Surgery. 2007;136(1):112-8. [DOI:10.1016/j.otohns.2006.07.002] [PMID]
4. Sharma P, Saxena S, Aggarwal P. Trends in the epidemiology of oral squamous cell carcinoma in Western UP: an institutional study. Indian Journal of Dental Research: official publication of Indian Society for Dental Research. 2010;21(3):316-9. [DOI:10.4103/0970-9290.70782] [PMID]
5. Tepper JE, Krasna MJ, Niedzwiecki D, Hollis D, Reed CE, Goldberg R, et al. Phase III trial of trimodality therapy with cisplatin, fluorouracil, radiotherapy, and surgery compared with surgery alone for esophageal cancer: CALGB 9781. Journal of clinical oncology. 2008;26(7):1086-92. [DOI:10.1200/JCO.2007.12.9593] [PMID] [PMCID]
6. Fiorica F, Di Bona D, Schepis F, Licata A, Shahied L, Venturi A, et al. Preoperative chemoradiotherapy for oesophageal cancer: a systematic review and meta-analysis. Gut. 2004;53(7):925-30. [DOI:10.1136/gut.2003.025080] [PMID] [PMCID]
7. Chu P, Wu E, Weiss LM. Cytokeratin 7 and cytokeratin 20 expression in epithelial eoplasms: survey of 435 cases. Modern Pathology. 2000;13(9):962-972. [DOI:10.1038/modpathol.3880175] [PMID]
8. Stopyra GA, Warhol MJ, Multhaupt HA. Cytokeratin 20 immunoreactivity in renal oncocytomas. The Journal of Histochemistry & Cytochemistry. 2001;49(7):919-920. [DOI:10.1177/002215540104900712] [PMID]
9. Chhieng DC, Cangiarella JF, Zakowski MF, Goswami S, Cohen JM, Yee HT. Use of thyroid transcription factor 1, PE‐10, and cytokeratins 7 and 20 in discriminating between primary lung carcinomas and metastatic lesions in fine‐needle aspiration biopsy specimens. Cancer Cytopathology. 2001;93(5):330-336. [DOI:10.1002/cncr.9048] [PMID]
10. Wang N, Zee S, Zarbo R, Bacchi C, Gown A. Coordinate expression of cytokeratins 7 and 20 defines unique subsets of carcinomas. Applied immunohistochemistry. 1995;3(2):99-107.
11. Nikitakis NG, Tosios KI, Papanikolaou VS, Rivera H, Papanicolaou SI, Ioffe OB. Immunohistochemical expression of cytokeratins 7 and 20 in malignant salivary gland tumors. Modern Pathology. 2004;17(4):407-415. [DOI:10.1038/modpathol.3800064] [PMID]
12. Mohajertehran F, Ayatollahi H, Khazaeni K, Shakeri M-T, Mohtasham N. Overexpression of high-mobility motor box 1 in the blood and tissues of patients with head and neck squamous cell carcinoma. Iranian journal of otorhinolaryngology. 2018;30(100):261-271.
13. Mohtasham N, Ayatollahi H, Saghravanian N, Zare R, Shakeri M, Sahebkar A, et al. Evaluation of Tissue and Serum Expression Levels of Lactate Dehydrogenase Isoenzymes in Patients with Head and Neck Squamous Cell Carcinoma. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry - Anti-Cancer Agents). 2019;19(17). [DOI:10.2174/1871520619666191014160818] [PMID]
14. Weinreb I, Tabanda-Lichauco R, Van der Kwast T, Perez-Ordoñez B. Low-grade intraductal carcinoma of salivary gland: report of 3 cases with marked apocrine differentiation. The American journal of surgical pathology. 2006;30(8):1014-1021. [DOI:10.1097/00000478-200608000-00013] [PMID]
15. Lee JH, Lee JH, Kim A, Kim I, Chae Ys. Unique expression of MUC3, MUC5AC and cytokeratins in salivary gland carcinomas. Pathology international. 2005;55(7):386-390. [DOI:10.1111/j.1440-1827.2005.01842.x] [PMID]
16. Terada T. Adenoid squamous cell carcinoma of the oral cavity. International journal of clinical and experimental pathology. 2012;5(5):442.
17. Silva DFB, Santos HBdP, León JE, Gomes DQdC, Alves PM, Nonaka CFW. Clinicopathological and immunohistochemical analysis of spindle cell squamous cell carcinoma of the tongue: a rare case. Einstein (São Paulo). 2019;17(1). [DOI:10.31744/einstein_journal/2019RC4610] [PMID] [PMCID]
18. Xie S, Bredell M, Yang H, Shen S, Yang H. Basaloid squamous cell carcinoma of the maxillary gingiva: a case report and review of the literature. Oncology letters. 2014;8(3):1287-1290. [DOI:10.3892/ol.2014.2318] [PMID] [PMCID]
19. Kusafuka K, Ebihara M, Ishiki H, Takizawa Y, Iida Y, Onitsuka T, et al. Primary adenoid squamous cell carcinoma of the oral cavity. Pathology international. 2006;56(2):78-83. [DOI:10.1111/j.1440-1827.2006.01927.x] [PMID]
20. Mestre VF, Medeiros‐Fonseca B, Estevao D, Casaca F, Silva S, Félix A, et al. HPV16 is sufficient to induce squamous cell carcinoma specifically in the tongue base in transgenic mice. The Journal of Pathology. 2020;251(1):4-11. [DOI:10.1002/path.5387] [PMID]
21. Abdul-Maksoud RS, Shalaby SM, Elsayed WS, Elkady S. Fibroblast growth factor receptor 1 and cytokeratin 20 expressions and their relation to prognostic variables in bladder cancer. Gene. 2016;591(2):320-326. [DOI:10.1016/j.gene.2016.05.043] [PMID]
22. Morsi MI, Youssef AI, Hassouna ME, El-Sedafi AS, Ghazal AA, Zaher ER. Telomerase activity, cytokeratin 20 and cytokeratin 19 in urine cells of bladder cancer patients. Journal of the Egyptian National Cancer Institute. 2006;18(1):82-92.

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