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Volume 10, Issue 3 (Vol.10 No.3 Oct 2021)
rbmb.net 2021, 10(3): 477-487 |
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Ilias A N, Ismail I S, Hamzah H, Mohd Mohidin T B, Idris M F, Ajat M. Rebaudioside A Enhances LDL Cholesterol Uptake in HepG2 Cells via Suppression of HMGCR Expression. rbmb.net 2021; 10 (3) :477-487
URL: http://rbmb.net/article-1-638-en.html
URL: http://rbmb.net/article-1-638-en.html
Amirul Nazhan Ilias 
, Intan Safinar Ismail , Hazilawati Hamzah , Taznim Begam Mohd Mohidin , Mohd Faiz Idris , Mokrish Ajat *
, Intan Safinar Ismail , Hazilawati Hamzah , Taznim Begam Mohd Mohidin , Mohd Faiz Idris , Mokrish Ajat *
Department of Veterinary Preclinical Science, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Selangor, Malaysia.
Abstract: (2664 Views)
Background: Rebaudioside A is one of the major diterpene glycosides found in Stevia had been reported to possess anti-hyperlipidemic effects. In this study, we explore the potential cholesterol-regulating mechanisms of Rebaudioside A in the human hepatoma (HepG2) cell line in comparison with simvastatin.
Methods: Cells were incubated with Rebaudioside A at several concentrations (0-10 μM) to determine the cytotoxicity by the MTT assay. Cells were treated with selected dosage (1 and 5 μM) in further experiments. Total cellular lipid was extracted by Bligh and Dyer method and subjected to quantitative colorimetric assay. To illustrate the effect of Rebaudioside A on cellular lipid droplets and low-density lipoprotein receptors, treated cells were subjected to immunofluorescence microscopy. Finally, we investigated the expression of experimental gene patterns of cells in response to treatment.
Results: In this study, cytotoxicity of Rebaudioside A was determined at 27.72 μM. Treatment of cells with a higher concentration of Rebaudioside A promotes better hepatocellular cholesterol internalization and ameliorates cholesterol-regulating genes such as HMGCR, LDLR, and ACAT2.
Conclusions: In conclusion, our data demonstrated that Rebaudioside A is capable to regulate cholesterol levels in HepG2 cells. Hence, we proposed that Rebaudioside A offers a potential alternative to statins for atherosclerosis therapy.
Methods: Cells were incubated with Rebaudioside A at several concentrations (0-10 μM) to determine the cytotoxicity by the MTT assay. Cells were treated with selected dosage (1 and 5 μM) in further experiments. Total cellular lipid was extracted by Bligh and Dyer method and subjected to quantitative colorimetric assay. To illustrate the effect of Rebaudioside A on cellular lipid droplets and low-density lipoprotein receptors, treated cells were subjected to immunofluorescence microscopy. Finally, we investigated the expression of experimental gene patterns of cells in response to treatment.
Results: In this study, cytotoxicity of Rebaudioside A was determined at 27.72 μM. Treatment of cells with a higher concentration of Rebaudioside A promotes better hepatocellular cholesterol internalization and ameliorates cholesterol-regulating genes such as HMGCR, LDLR, and ACAT2.
Conclusions: In conclusion, our data demonstrated that Rebaudioside A is capable to regulate cholesterol levels in HepG2 cells. Hence, we proposed that Rebaudioside A offers a potential alternative to statins for atherosclerosis therapy.
Keywords: Rebaudioside A, Anti-hypercholesterolemia, Lipid droplets, Low-density lipoprotein, HMGCR.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2021/01/13 | Accepted: 2021/02/8 | Published: 2021/12/5
Received: 2021/01/13 | Accepted: 2021/02/8 | Published: 2021/12/5
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