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Volume 10, Issue 2 (Vol.10 No.2 Jul 2021)
rbmb.net 2021, 10(2): 183-196 |
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Mohammadi A, Balizadeh Karami A R, Dehghan Mashtani V, Sahraei T, Bandani Tarashoki Z, Khattavian E, et al . Evaluation of Oxidative Stress, Apoptosis, and Expression of MicroRNA-208a and MicroRNA-1 in Cardiovascular Patients. rbmb.net 2021; 10 (2) :183-196
URL: http://rbmb.net/article-1-645-en.html
URL: http://rbmb.net/article-1-645-en.html
Asma Mohammadi 
, Ali Reza Balizadeh Karami , Vahid Dehghan Mashtani , Tooba Sahraei , Zeinab Bandani Tarashoki , Ehsan Khattavian , Sara Mobarak , Hossein Moradi Kazerouni , Esmat Radmanesh *
, Ali Reza Balizadeh Karami , Vahid Dehghan Mashtani , Tooba Sahraei , Zeinab Bandani Tarashoki , Ehsan Khattavian , Sara Mobarak , Hossein Moradi Kazerouni , Esmat Radmanesh *
Abadan Faculty of Medical Sciences, Abadan, Iran & Student Research Committee, Abadan Faculty of Medical Sciences, Abadan, Iran.
Abstract: (4466 Views)
Background: MicroRNA expression signature and reactive oxygen species (ROS) production have been associated with the development of cardiovascular diseases (CVDs). This study aimed to evaluate oxidative stress, inflammation, apoptosis, and the expression of miRNA-208a and miRNA-1 in cardiovascular patients.
Methods: The study population included four types of patients (acute coronary syndromes (ACS), myocardial infarction (MI), arrhythmia, and heart failure (HF)), with 10 people in each group, as well as a control group. Quantitative real-time PCR was performed to measure mir-208 and miR-1 expression, the mRNAs of inflammatory mediators (TNFα, iNOS/eNOS), and apoptotic factors (Bax and Bcl2). XOX, MDA, and antioxidant enzymes (CAT, SOD, and GPx) were measured by ZellBio GmbH kits by an ELISA Reader.
Results: The results showed significant decreases in the activity of antioxidant enzymes (CAT, SOD, and Gpx) and a significant increase in the activity of the MDA and XOX in cardiovascular patients. Significant increases in IL-10, iNos, iNOS / eNOS, and TNF-α in cardiovascular patients were also observed. Also, a significant increase in the expression of miR-208 (HF> arrhythmia> ACS> MI) and a significant decrease in the expression of miR-1 (ACS> arrhythmia> HF> MI) were found in all four groups in cardiovascular patients.
Conclusions: The results showed increases in oxidative stress, inflammation, apoptotic factors, and in the expression of miR-208a in a variety of cardiovascular patients (ACS, MI, arrhythmia, and HF). It is suggested that future studies determine the relationships that miR-1, miR-208, and oxidative stress indices have with inflammation and apoptosis.
Methods: The study population included four types of patients (acute coronary syndromes (ACS), myocardial infarction (MI), arrhythmia, and heart failure (HF)), with 10 people in each group, as well as a control group. Quantitative real-time PCR was performed to measure mir-208 and miR-1 expression, the mRNAs of inflammatory mediators (TNFα, iNOS/eNOS), and apoptotic factors (Bax and Bcl2). XOX, MDA, and antioxidant enzymes (CAT, SOD, and GPx) were measured by ZellBio GmbH kits by an ELISA Reader.
Results: The results showed significant decreases in the activity of antioxidant enzymes (CAT, SOD, and Gpx) and a significant increase in the activity of the MDA and XOX in cardiovascular patients. Significant increases in IL-10, iNos, iNOS / eNOS, and TNF-α in cardiovascular patients were also observed. Also, a significant increase in the expression of miR-208 (HF> arrhythmia> ACS> MI) and a significant decrease in the expression of miR-1 (ACS> arrhythmia> HF> MI) were found in all four groups in cardiovascular patients.
Conclusions: The results showed increases in oxidative stress, inflammation, apoptotic factors, and in the expression of miR-208a in a variety of cardiovascular patients (ACS, MI, arrhythmia, and HF). It is suggested that future studies determine the relationships that miR-1, miR-208, and oxidative stress indices have with inflammation and apoptosis.
Keywords: Apoptosis, Cardiovascular diseases, Inflammation, microRNA-208a, microRNA-1, Oxidative stress.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2021/01/23 | Accepted: 2021/02/15 | Published: 2021/08/26
Received: 2021/01/23 | Accepted: 2021/02/15 | Published: 2021/08/26
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