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Volume 10, Issue 3 (Vol.10 No.3 Oct 2021)
rbmb.net 2021, 10(3): 362-372 |
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Ghanem H B, Emam M N, Ali D A M, Abd-Ellatif R N. Impact of Berberine on Some Epigenetic, Transcription Regulation and Inflammatory Biomarkers in a Mice Model of Epilepsy. rbmb.net 2021; 10 (3) :362-372
URL: http://rbmb.net/article-1-660-en.html
URL: http://rbmb.net/article-1-660-en.html
Clinical laboratory sciences Department, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia & Medical Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Abstract: (2778 Views)
Background: Epilepsy is one of the most widespread neurological disease worldwide. Status epilepticus (SE) is a life-threatening neurologic disorder. Neuroprotective approaches are increasingly to discover a promising therapy to manage epileptic disorders. This study aimed to assess the impact of berberine on some epigenetic, transcription regulation & inflammatory biomarkers in a mice model of epilepsy.
Methods: This work was performed on; Group I: (control), Group II: berberine‐treated control, Group III: epilepsy group, Group IV: berberine‐treated epilepsy. Groups were subjected to assessment of Tumor growth factor-1ꞵ (TGF-1ꞵ), hypoxia inducible factor-1α (HIF-1α), brain derived neurotrophic factor (BDNF) levels, histone deacetylase (HDAC) activity & neuronal restrictive silencing factor (NRSF) gene expression.
Results: Study showed significant increase in levels of HIF-1α, TGF-1β, HDAC activity & NRSF gene expression in epilepsy group & decrease in these levels in berberine treated epilepsy group. Significant decrease in BDNF levels in epilepsy & elevation in them in berberine treated epilepsy group.
Conclusions: Our study showed the anti-epileptic impact of berberine via its regulatory effect on some epigenetic, transcription factors & inflammatory biomarkers in a mice model of epilepsy.
Methods: This work was performed on; Group I: (control), Group II: berberine‐treated control, Group III: epilepsy group, Group IV: berberine‐treated epilepsy. Groups were subjected to assessment of Tumor growth factor-1ꞵ (TGF-1ꞵ), hypoxia inducible factor-1α (HIF-1α), brain derived neurotrophic factor (BDNF) levels, histone deacetylase (HDAC) activity & neuronal restrictive silencing factor (NRSF) gene expression.
Results: Study showed significant increase in levels of HIF-1α, TGF-1β, HDAC activity & NRSF gene expression in epilepsy group & decrease in these levels in berberine treated epilepsy group. Significant decrease in BDNF levels in epilepsy & elevation in them in berberine treated epilepsy group.
Conclusions: Our study showed the anti-epileptic impact of berberine via its regulatory effect on some epigenetic, transcription factors & inflammatory biomarkers in a mice model of epilepsy.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2021/02/20 | Accepted: 2021/03/15 | Published: 2021/12/5
Received: 2021/02/20 | Accepted: 2021/03/15 | Published: 2021/12/5
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