Volume 4, Issue 1 (Vol.4 No.1 Oct 2015)                   rbmb.net 2015, 4(1): 32-42 | Back to browse issues page

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Sedighi M, Moghoofei M, Kouhsari E, Pournajaf A, Emadi B, Tohidfar M et al . In silico Analysis and Molecular Modeling of RNA Polymerase, Sigma S (RpoS) Protein in Pseudomonas aeruginosa PAO1. rbmb.net 2015; 4 (1) :32-42
URL: http://rbmb.net/article-1-69-en.html
Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Abstract:   (7871 Views)

Background: Sigma factors are proteins that regulate transcription in bacteria. Sigma factors can be activated in response to different environmental conditions. The rpoS (RNA polymerase, sigma S) gene encodes sigma-38 (σ38, or RpoS), a 37.8 kDa protein in Pseudomonas aeruginosa (P. aeruginosa) strains. RpoS is a central regulator of the general stress response and operates in both retroactive and proactive manners; not only does it allow the cell to survive environmental challenges; it also prepares the cell for subsequent stresses (cross-protection).

Methods: The significance of RpoS for stress resistance and protein expression in stationary-phase P. aeruginosa cells was assessed. The goal of the current study was to characterize RpoS of P. aeruginosa PAO1 using bioinformatics tools.

Results: The results showed that RpoS is an unstable protein that belongs to the sigma-70 factor family. Secondary structure analysis predicted that random coil is the predominant structure followed by extended alpha helix. The three-dimensional (3D) structure was modeled using SWISS-MODEL Workspace.

Conclusion: Determination of sequence, function, structure, and predicted epitopes of RpoS is important for modeling of inhibitors that will help in the design of new drugs to combat multi-drug-resistant (MDR) strains. Such information may aid in the development of new diagnostic tools, drugs, and vaccines for treatment in endemic regions.

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Subject: Biochemistry
Received: 2015/01/7 | Accepted: 2015/02/20 | Published: 2015/10/30

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