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Al-Kawaz H S, Yasser O M, Jaafar Mousa M. A New Method to Estimate Inhibition Percentage of Endogenous Digitalis in Patients with Pre-eclampsia. rbmb.net. 2022; 11 (1) :138-145
URL: http://rbmb.net/article-1-803-en.html
Department of Medical Laboratories Techniques, Al-Mustaqbal University College, Babylon, Iraq.
Abstract:   (610 Views)
Background: Pre-eclampsia is an idiopathic pregnancy disorder characterized by appearance proteinuria and hypertension, with poorly understood etiology. It has been linked to a variety of system abnormalities, including ion transport disorders in neonatal, maternal, and placental cell lines. A new method was described to evaluate the inhibition percentage of endogenous digitalis in plasma of pre-eclampsia patients compared with normal pregnancies, with the estimation of sensitivity and specificity of the proposed test.

Methods: This was a case-control study consisting of 130 cases that were divided into three groups, 55 normal pregnancies (positive control), 30 non-pregnant women (negative control), and 45 preeclampsia (patients). The new method included the estimation of the percentage inhibition of endogenous digitalis by measuring specific enzyme activity of Na-K ATPase for the patient and positive control. The results were analyzed using the statistical package for social sciences (SPSS®) software version 26.0. A p-value of≤ 0.05 was considered significant.

Results: In the pre-eclampsia patient, the specific activity of Na-K ATPase was significantly lower with mean= 0.239 mg/g±0.043 compared to the control group which was 0.397 mg/g±0.021, p<0.001. While the result of inhibition percentage of endogenous digitalis showed significantly higher in the pre-eclampsia patient (mean= 35.852 mg/g %±2.692%) compared to the control group (mean=17.964%±1.784), with a p< 0.001.

Conclusions: Pre-eclampsia is linked with lower erythrocyte sodium pump activity significantly in pre-eclampsia patients than in normal pregnancies. Also, results show the inhibited percentage of endogenous digitalis elevation in patients with pre-eclampsia compared with normal pregnancy.
Full-Text [PDF 279 kb]   (310 Downloads)    
Type of Article: Original Article | Subject: Biochemistry
Received: 2021/10/21 | Accepted: 2021/11/29 | Published: 2022/05/26

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