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Al-Kawaz H S, Yasser O M, Jaafar Mousa M. A New Method to Estimate Inhibition Percentage of Endogenous Digitalis in Patients with Pre-eclampsia. rbmb.net 2022; 11 (1) :138-145
URL: http://rbmb.net/article-1-803-en.html
URL: http://rbmb.net/article-1-803-en.html
Department of Medical Laboratories Techniques, Al-Mustaqbal University College, Babylon, Iraq.
Abstract: (2332 Views)
Background: Pre-eclampsia is an idiopathic pregnancy disorder characterized by appearance proteinuria and hypertension, with poorly understood etiology. It has been linked to a variety of system abnormalities, including ion transport disorders in neonatal, maternal, and placental cell lines. A new method was described to evaluate the inhibition percentage of endogenous digitalis in plasma of pre-eclampsia patients compared with normal pregnancies, with the estimation of sensitivity and specificity of the proposed test.
Methods: This was a case-control study consisting of 130 cases that were divided into three groups, 55 normal pregnancies (positive control), 30 non-pregnant women (negative control), and 45 preeclampsia (patients). The new method included the estimation of the percentage inhibition of endogenous digitalis by measuring specific enzyme activity of Na-K ATPase for the patient and positive control. The results were analyzed using the statistical package for social sciences (SPSS®) software version 26.0. A p-value of≤ 0.05 was considered significant.
Results: In the pre-eclampsia patient, the specific activity of Na-K ATPase was significantly lower with mean= 0.239 mg/g±0.043 compared to the control group which was 0.397 mg/g±0.021, p<0.001. While the result of inhibition percentage of endogenous digitalis showed significantly higher in the pre-eclampsia patient (mean= 35.852 mg/g %±2.692%) compared to the control group (mean=17.964%±1.784), with a p< 0.001.
Conclusions: Pre-eclampsia is linked with lower erythrocyte sodium pump activity significantly in pre-eclampsia patients than in normal pregnancies. Also, results show the inhibited percentage of endogenous digitalis elevation in patients with pre-eclampsia compared with normal pregnancy.
Methods: This was a case-control study consisting of 130 cases that were divided into three groups, 55 normal pregnancies (positive control), 30 non-pregnant women (negative control), and 45 preeclampsia (patients). The new method included the estimation of the percentage inhibition of endogenous digitalis by measuring specific enzyme activity of Na-K ATPase for the patient and positive control. The results were analyzed using the statistical package for social sciences (SPSS®) software version 26.0. A p-value of≤ 0.05 was considered significant.
Results: In the pre-eclampsia patient, the specific activity of Na-K ATPase was significantly lower with mean= 0.239 mg/g±0.043 compared to the control group which was 0.397 mg/g±0.021, p<0.001. While the result of inhibition percentage of endogenous digitalis showed significantly higher in the pre-eclampsia patient (mean= 35.852 mg/g %±2.692%) compared to the control group (mean=17.964%±1.784), with a p< 0.001.
Conclusions: Pre-eclampsia is linked with lower erythrocyte sodium pump activity significantly in pre-eclampsia patients than in normal pregnancies. Also, results show the inhibited percentage of endogenous digitalis elevation in patients with pre-eclampsia compared with normal pregnancy.
Type of Article: Original Article |
Subject:
Biochemistry
Received: 2021/10/21 | Accepted: 2021/11/29 | Published: 2022/05/26
Received: 2021/10/21 | Accepted: 2021/11/29 | Published: 2022/05/26
References
1. Koushki M, Amiri Dash Atan N, Omidi-Ardali H, Rezaei Tavirani M. Assessment of Correlation Between miR-210 Expression and Pre-Eclampsia Risk: A Meta-Analysis. Rep Biochem Mol Biol. 2018;7(1):94-101.
2. Hung TH, Burton GJ. Hypoxia and reoxygenation: A possible mechanism for placental oxidative stress in preeclampsia. Taiwan J Obstet Gynecol. 2006;45(3):189-200. [DOI:10.1016/S1028-4559(09)60224-2]
3. Hacker NF, Gambone JC, Hobel CJ. Hacker & Moore's essentials of obstetrics and gynecology. Elsevier Health Sciences; 2015.
4. Akter K, Khanum H. Prevalence of Pre-Eclampsia and Factors Responsible among Third Trimester Pregnant Women in Hospital of Dhaka. Biomedical Journal of Scientific & Technical Research. 2021;33(4):26089-26097. [DOI:10.26717/BJSTR.2021.33.005445]
5. Serrano Cardona L, Muñoz Mata E. Paraninfo digital. Early Hum Dev. 2013;83:1-1.
6. Schoner W, Scheiner-Bobis G. Endogenous and exogenous cardiac glycosides: Their roles in hypertension, salt metabolism, and cell growth. Am J Physiol Cell Physiol. 2007;293(2):C509-36. [DOI:10.1152/ajpcell.00098.2007] [PMID]
7. Adair CD, Haupert GT, Koh HP, Wang Y, Veille JC, Buckalew V. Erythrocyte sodium/potassium ATPase activity in severe preeclampsia. Journal of Perinatology. 2009;29(4):280-283. [DOI:10.1038/jp.2008.223] [PMID] [PMCID]
8. Fedorova OV, Shapiro JI, Bagrov AY. Endogenous cardiotonic steroids and salt-sensitive hypertension. Basis of Disease. 2010;1802(12):1230-1236. [DOI:10.1016/j.bbadis.2010.03.011] [PMID] [PMCID]
9. Słabiak-Błaż N, Piecha G. Endogenous mammalian cardiotonic steroids-a new cardiovascular risk factor? a mini-review. Life (Basel). 2021;11(8):727. [DOI:10.3390/life11080727] [PMID] [PMCID]
10. Bagrov AY, Shapiro JI, Fedorova OV. Endogenous cardiotonic steroids: physiology, pharmacology, and novel therapeutic targets. Pharmacological Reviews. 2009;61(1):9-38. [DOI:10.1124/pr.108.000711] [PMID] [PMCID]
11. Murrell JR, Randall JD, Rosoff J, Zhao JL, Jensen RV, Gullans SR, et al. Endogenous ouabain: Upregulation of steroidogenic genes in hypertensive hypothalamus but not adrenal. Circulation. 2005;112(9):1301-8. [DOI:10.1161/CIRCULATIONAHA.105.554071] [PMID]
12. Gallery E DM, Hunyor SN, Györy AZ. Plasma volume contraction: A significant factor in both pregnancy-associated hypertension (preeclampsia) and chronic hypertension in pregnancy. Obstetrical and Gynecological Survey. 1980;35(9):557-558. [DOI:10.1097/00006254-198009000-00007]
13. Graves SW. The possible role of digitalis-like factors in pregnancy-induced hypertension. Hypertension. 1987;10(5):I84-I86. [DOI:10.1161/01.HYP.10.5_Pt_2.I84] [PMID]
14. Graves SW, Eder JP, Schryber SM, Sharma K, Brena A, Antman KH, et al. Endogenous digoxin-like immunoreactive factor and digitalis-like factor associated with the hypertension of patients receiving multiple alkylating agents as part of autologous bone marrow transplantation. Clin Sci (Lond). 1989;77(5):501-7. [DOI:10.1042/cs0770501] [PMID]
15. Paczula A, Wiȩcek A, Piecha G. The role of endogenous cardiotonic steroids in pathogenesis of cardiovascular and renal complications of arterial hypertension. Postepy Hig Med Dosw (Online). 2016;70:243-50. [DOI:10.5604/17322693.1197486] [PMID]
16. Al-Jameil N, Tabassum H, Al-Mayouf H, Aljohar HI, Alenzi ND, Hijazy SM, et al. FAAnalysis of serum trace elements-copper, manganese and zinc in preeclamptic pregnant women by inductively coupled plasma optical emission spectrometry: A prospective case-controlled study in Riyadh, Saudi Arabia. Int J Clin Exp Pathol. 2014;7(5):1900-1910.
17. Gathiram P, Moodley J. Pre-eclampsia: Its pathogenesis and pathophysiology. Cardiovasc J Afr. 2016;27(2):71-78. [DOI:10.5830/CVJA-2016-009] [PMID] [PMCID]
18. Witlin AG, Saade GR, Mattar F, Sibai BM. Predictors of neonatal outcome in women with severe preeclampsia or eclampsia between 24 and 33 weeks' gestation. Am J Obstet Gynecol. 2000;182(3):607-11. [DOI:10.1067/mob.2000.104224] [PMID]
19. Masoumi E, Mirzaei A, Ghaffari-Nazari H, Tahaghoghi-Hajghorbani S, Jalali SA, Tavakkol-Afshari J. Serum Levels of Soluble Fas and Fas Ligand in Iranian Women with Pre-Eclampsia. Rep Biochem Mol Biol. 2021;9(4):394-398. [DOI:10.52547/rbmb.9.4.394] [PMID] [PMCID]
20. Kumar SG, Unnikrishnan B, Nagaraj K, Jayaram S. Determinants of pre-eclampsia: A case-control study in a district hospital in South India. Indian J Community Med. 2010;35(4):502-505. [DOI:10.4103/0970-0218.74360] [PMID] [PMCID]
21. Darkwa EO, Djagbletey R, Aryee CA-BG, Sottie D, AA. Serum sodium and potassium levels in preeclampsia: A case-control study in a large tertiary hospital in Ghana. Cogent Medicine. 2017;4(1):1-10. [DOI:10.1080/2331205X.2017.1376898]
22. James PR, Nelson-Piercy C. Management of hypertension before, during, and after pregnancy. Heart. 2004;90(12):1499-504. [DOI:10.1136/hrt.2004.035444] [PMID] [PMCID]
23. Xiong X, Demianczuk NN, Buekens P, Saunders LD. Association of preeclampsia with high birth weight for age. American Journal of Obstetrics and Gynecology. 2000;183(1):148-155.
https://doi.org/10.1067/mob.2000.105735 [DOI:10.1016/S0002-9378(00)24793-5] [PMID]
24. Malfatti C RM, Burgos LT, Rieger A, Rüdger CL, Túrmina JA, et al. Decreased erythrocyte NA+, K+ -ATPase activity and increased plasma TBARS in prehypertensive patients The Scientific World Journal. 2012; (2012); 1-5. [DOI:10.1100/2012/348246] [PMID] [PMCID]
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