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Al-Fahad D, Alyaseen F, Al-Amery A, Singh G, Srinath M, Abbas Y, et al . Kinetic Changes of Ptdins (3,4,5) P3 within Fast and Slow Turnover Rates of Focal Adhesion. rbmb.net 2022; 11 (2) :262-269
URL: http://rbmb.net/article-1-849-en.html
Kinetic Changes of Ptdins (3,4,5) P3 within Fast and Slow Turnover Rates of Focal Adhesion
Dhurgham Al-Fahad * , Firas Alyaseen , Ahmed Al-Amery , Gagandeep Singh , Mote Srinath , Yahya Abbas , Hafiz Muzzammel Rehman , Yahya Abbas
Department of Pharmaceutical Sciences, College of Pharmacy University of Thi-Qar, Thi-Qar 64001, Iraq.
Abstract:   (2481 Views)
Background: The assembly and disassembly of the focal adhesions (FA) components occurs throughout life cycle of adhesion, with conservation of balance between removal and recruitment rate during temporal stages. Previous studies have demonstrated that phosphotidyilinositols play a role in regulating FA turnover. However, a little attention has been given to quantify the dynamics changes of Phosphatidylinositol 3,4,5-trisphosphate (PtdIns (3,4,5) P3) within and during fast and slow turnover rates of FA.

Methods: MDA-MB-231 breast cancer cell line was used as a model in this study due to high metastatic and motile. These cells were co-transfected with GFP- paxillin/vinculin, as FA marker, and the GFP/mCherry-Btk-PH, as a biosensor to visualize PtdIns (3,4,5) P3. Confocal time-lapse images were used
to monitor changes or differences in the local generation of PtdIns (3,4,5) P3 within and during assembly and disassembly of FA. Following transfection, immunostaining was used to examine the spatial colocalization between FA and PtdIns (3,4,5) P3.

Results: Our data demonstrated that PtdIns (3,4,5) P3 co-localized with FAs and increase during assembly and decline during disassembly of FA which exhibits slow turnover rates and was in a constant level during assembly and disassembly of FA that displays fast turnover rates. 

Conclusions: Our result suggested that the dynamic changes of PtdIns (3,4,5) P3, it may depend on components undergo turnover, such that early, nascent FA displays fast turnover rates and mature FA exhibits slow turnover rates. Thus, the local enrichment of PtdIns (3,4,5) P3 enhances FA assembly and disassembly activation.
Keywords: Cancer cell migration, Focal adhesion turnover, MDA-MB-231 cell line, PtdIns (3, 4, 5) P3.
Full-Text [PDF 280 kb]   (1552 Downloads)    
Type of Article: Original Article | Subject: Cell Biology
Received: 2022/01/2 | Accepted: 2022/01/2 | Published: 2022/08/7
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