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Nour Z, Elhamamsy K, Ehsan I, Fawaz L, Shaker O, Mossallam D et al . MicroRNAs as Potential Diagnostic New Biomarkers in Diagnosis of Sepsis in Pediatric Patients. rbmb.net 2022; 11 (2) :327-335
URL: http://rbmb.net/article-1-889-en.html
URL: http://rbmb.net/article-1-889-en.html
Zeinab Nour * 
, Kariman Elhamamsy , Iman Ehsan , Lobna Fawaz , Olfat Shaker , Dalia Mossallam , Hala ElGindy
, Kariman Elhamamsy , Iman Ehsan , Lobna Fawaz , Olfat Shaker , Dalia Mossallam , Hala ElGindy
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract: (1880 Views)
Background: Sepsis is one of the most common causes of multiorgan failure. Sepsis requires the presence of infection with a resultant systemic inflammatory state. Organ dysfunction occurs from the combination of the two processes. Sepsis is the main cause of mortality at intensive care units, with 30-50% mortality rate for all septic episodes. MicroRNA (miRNA) profile data could be beneficial as a specific diagnostic biomarker for sepsis and systemic inflammatory response syndrome (SIRS).
Methods: Expression of miRNAs -122, -181b, -223 and -146a levels were assayed by quantitative real time polymerase chain reaction (qRT-PCR) in a prospective case control study, where forty septic cases were compared to 40 healthy controls of matched age and gender.
Results: miRNAs -122 and -181b were significantly upregulated during early septic conditions, indicating that they could be sensitive and specific biomarkers for diagnosing sepsis. miRNA-223 and miRNA-146a could also represent highly specific and sensitive diagnostic biomarkers, as they were found to be significantly down-regulated. Serum levels of miRNA-223 could be used to predict poor prognosis with 70% sensitivity and 75% specificity, whereas the other three miRNAs could not predict prognosis.
Conclusions: Our study shows that all tested miRNAs can be used for early detection of sepsis, with miRNA-223 being predictive of mortality, hence preventing multi-organ failure and reducing mortality, and predicting poor outcomes, thereby assisting in early categorization of ICU patients for rapid appropriate treatment and medico legal aspects.
Methods: Expression of miRNAs -122, -181b, -223 and -146a levels were assayed by quantitative real time polymerase chain reaction (qRT-PCR) in a prospective case control study, where forty septic cases were compared to 40 healthy controls of matched age and gender.
Results: miRNAs -122 and -181b were significantly upregulated during early septic conditions, indicating that they could be sensitive and specific biomarkers for diagnosing sepsis. miRNA-223 and miRNA-146a could also represent highly specific and sensitive diagnostic biomarkers, as they were found to be significantly down-regulated. Serum levels of miRNA-223 could be used to predict poor prognosis with 70% sensitivity and 75% specificity, whereas the other three miRNAs could not predict prognosis.
Conclusions: Our study shows that all tested miRNAs can be used for early detection of sepsis, with miRNA-223 being predictive of mortality, hence preventing multi-organ failure and reducing mortality, and predicting poor outcomes, thereby assisting in early categorization of ICU patients for rapid appropriate treatment and medico legal aspects.
Keywords: Systemic inflammatory response syndrome (SIRS), Sepsis, Mortality, Biomarker, micRNA-223, Prognostic factor
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2022/03/7 | Accepted: 2022/03/8 | Published: 2022/08/7
Received: 2022/03/7 | Accepted: 2022/03/8 | Published: 2022/08/7
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