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Volume 11, Issue 2 (Vol.11 No.2 Jul 2022)
rbmb.net 2022, 11(2): 336-343 |
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Sardarzade N, khojasteh-Leylakoohi F, Damavandi S, Khalili-Tanha G, Dashtiahangar M, Khalili-Tanha N, et al . Association of a Genetic Variant in the Cyclin-Dependent Kinase Inhibitor 2B with Risk of Pancreatic Cancer. rbmb.net 2022; 11 (2) :336-343
URL: http://rbmb.net/article-1-895-en.html
URL: http://rbmb.net/article-1-895-en.html
Newsha Sardarzade 
, Fatemeh Khojasteh-Leylakoohi , Sedigheh Damavandi , Ghazaleh Khalili-Tanha , Mohammad Dashtiahangar , Nima Khalili-Tanha , Amir Avan , Sakineh Amoueian , Seyed Mahdi Hassanian , Habibollah Esmaily , Majid Khazaei , Gordon Ferns , Alireza Khooei , Mohsen Aliakbarian *
, Fatemeh Khojasteh-Leylakoohi , Sedigheh Damavandi , Ghazaleh Khalili-Tanha , Mohammad Dashtiahangar , Nima Khalili-Tanha , Amir Avan , Sakineh Amoueian , Seyed Mahdi Hassanian , Habibollah Esmaily , Majid Khazaei , Gordon Ferns , Alireza Khooei , Mohsen Aliakbarian *
Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Abstract: (2402 Views)
Background: Pancreatic cancer (PC) is among the most aggressive tumors with a poor prognosis, indicating the need for the identification of a novel prognostic biomarker for risk stratifications. Recent genome-wide association studies have demonstrated common genetic variants in a region on chromosome 9p21 associated with an increased risk of different malignancies.
Methods: In the present study, we explore the possible relationship between genetic variant, rs10811661, and gene expression of CDKN2B in 75 pancreatic cancer patients, and 188 healthy individuals. DNAs were extracted and genotyping and gene expression were performed by TaqMan real-time PCR and RT-PCR, respectively. Logistic regression was used to assess the association between risk and genotypes, while the significant prognostic variables in the univariate analysis were included in multivariate analyses.
Results: The patients with PDAC had a higher frequency of a TT genotype for rs10811661 than the control group. Also, PDAC patients with dominant genetic model, (TT + TC), was associated with increased risk of developing PDAC (OR= 14.71, 95% CI [1.96-110.35], p= 0.009). Moreover, patients with CC genotype had a higher expression of CDKN2B, in comparison with TT genotype.
Conclusions: Our findings demonstrated that CDKN2A/B was associated with the risk of developing PDAC, supporting further investigations in the larger and multicenter setting to validate the potential value of this gene as an emerging marker for PDAC.
Methods: In the present study, we explore the possible relationship between genetic variant, rs10811661, and gene expression of CDKN2B in 75 pancreatic cancer patients, and 188 healthy individuals. DNAs were extracted and genotyping and gene expression were performed by TaqMan real-time PCR and RT-PCR, respectively. Logistic regression was used to assess the association between risk and genotypes, while the significant prognostic variables in the univariate analysis were included in multivariate analyses.
Results: The patients with PDAC had a higher frequency of a TT genotype for rs10811661 than the control group. Also, PDAC patients with dominant genetic model, (TT + TC), was associated with increased risk of developing PDAC (OR= 14.71, 95% CI [1.96-110.35], p= 0.009). Moreover, patients with CC genotype had a higher expression of CDKN2B, in comparison with TT genotype.
Conclusions: Our findings demonstrated that CDKN2A/B was associated with the risk of developing PDAC, supporting further investigations in the larger and multicenter setting to validate the potential value of this gene as an emerging marker for PDAC.
Type of Article: Original Article |
Subject:
Molecular Biology
Received: 2022/03/23 | Accepted: 2022/03/27 | Published: 2022/08/7
Received: 2022/03/23 | Accepted: 2022/03/27 | Published: 2022/08/7
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